Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule
The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up‐regulated by agr whereas surface proteins are down‐regulated. agr contains two divergent promoters, one of which directs the...
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| Veröffentlicht in: | The EMBO journal 1993-10, Vol.12 (10), p.3967-3975 |
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| container_title | The EMBO journal |
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| creator | Novick, R.P. Ross, H.F. Projan, S.J. Kornblum, J. Kreiswirth, B. Moghazeh, S. |
| description | The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up‐regulated by agr whereas surface proteins are down‐regulated. agr contains two divergent promoters, one of which directs the synthesis of a 514 nucleotide (nt) transcript, RNAIII. In this report, we show that the cloned RNAIII determinant restores both positive and negative regulatory functions of agr to an agr‐null strain and that the RNA itself, rather than any protein, is the effector molecule. RNAIII acts primarily on the initiation of transcription and, secondarily in some cases, at the level of translation. In these cases, translation and transcription are regulated independently. RNAIII probably regulates translation directly by interacting with target gene transcripts and transcription indirectly by means of intermediary protein factors. |
| doi_str_mv | 10.1002/j.1460-2075.1993.tb06074.x |
| format | Article |
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Secretory proteins are up‐regulated by agr whereas surface proteins are down‐regulated. agr contains two divergent promoters, one of which directs the synthesis of a 514 nucleotide (nt) transcript, RNAIII. In this report, we show that the cloned RNAIII determinant restores both positive and negative regulatory functions of agr to an agr‐null strain and that the RNA itself, rather than any protein, is the effector molecule. RNAIII acts primarily on the initiation of transcription and, secondarily in some cases, at the level of translation. In these cases, translation and transcription are regulated independently. RNAIII probably regulates translation directly by interacting with target gene transcripts and transcription indirectly by means of intermediary protein factors.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1993.tb06074.x</identifier><identifier>PMID: 7691599</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; Down-Regulation ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Conformation ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Promoter Regions, Genetic ; Protein Biosynthesis ; Restriction Mapping ; RNA, Bacterial - chemistry ; RNA, Bacterial - metabolism ; Staphylococcus aureus - genetics ; Staphylococcus aureus - pathogenicity ; Transcription, Genetic ; Up-Regulation ; Virulence</subject><ispartof>The EMBO journal, 1993-10, Vol.12 (10), p.3967-3975</ispartof><rights>1993 European Molecular Biology Organization</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6614-4917ec0d3f9f876c3798b8cd5c7bc933e3f0056f8da040fca465645b581caeae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413679/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413679/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4909675$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7691599$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Novick, R.P.</creatorcontrib><creatorcontrib>Ross, H.F.</creatorcontrib><creatorcontrib>Projan, S.J.</creatorcontrib><creatorcontrib>Kornblum, J.</creatorcontrib><creatorcontrib>Kreiswirth, B.</creatorcontrib><creatorcontrib>Moghazeh, S.</creatorcontrib><title>Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up‐regulated by agr whereas surface proteins are down‐regulated. agr contains two divergent promoters, one of which directs the synthesis of a 514 nucleotide (nt) transcript, RNAIII. In this report, we show that the cloned RNAIII determinant restores both positive and negative regulatory functions of agr to an agr‐null strain and that the RNA itself, rather than any protein, is the effector molecule. RNAIII acts primarily on the initiation of transcription and, secondarily in some cases, at the level of translation. In these cases, translation and transcription are regulated independently. RNAIII probably regulates translation directly by interacting with target gene transcripts and transcription indirectly by means of intermediary protein factors.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Down-Regulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Biosynthesis</subject><subject>Restriction Mapping</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - metabolism</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Transcription, Genetic</subject><subject>Up-Regulation</subject><subject>Virulence</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV1rFDEYhYNY6lr9CUIQ8W7GZJNJJoIXa6kfpSr4Ad6FzDtJd5bsZE1mauffm3WHpb0SrxI4zwknPAg9p6SkhCxfbUrKBSmWRFYlVYqVQ0MEkby8fYAWx-ghWpCloAWntXqEHqe0IYRUtaSn6FQKRSulFujnt6kf1jZ1CQeH02B268kHCADG45sujt72YLEzMISYcMYg9EMM3tsWNxM2ONrr0ZucTvjr5xXeBm8ht56gE2d8sk_n8wz9eHfx_fxDcfXl_cfz1VUBQlBecEWlBdIyp1wtBTCp6qaGtgLZgGLMMpdHC1e3hnDiwHBRCV41VU3BWGPZGXpzeHc3Nlvbgs3rjNe72G1NnHQwnb6f9N1aX4cbzSkTUuX-y7kfw6_RpkFvuwTWe9PbMCYtK1ULJtg_QSqEzD_iGXx9ACGGlKJ1xzGU6L0_vdF7SXovSe_96dmfvs3lZ3e_c6zOwnL-Ys5NyopcND106YhxRZSQVcZWB-x35-30HwP0xae3l3_v7A-9Wbrq</recordid><startdate>199310</startdate><enddate>199310</enddate><creator>Novick, R.P.</creator><creator>Ross, H.F.</creator><creator>Projan, S.J.</creator><creator>Kornblum, J.</creator><creator>Kreiswirth, B.</creator><creator>Moghazeh, S.</creator><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199310</creationdate><title>Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule</title><author>Novick, R.P. ; Ross, H.F. ; Projan, S.J. ; Kornblum, J. ; Kreiswirth, B. ; Moghazeh, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6614-4917ec0d3f9f876c3798b8cd5c7bc933e3f0056f8da040fca465645b581caeae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Down-Regulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Biosynthesis</topic><topic>Restriction Mapping</topic><topic>RNA, Bacterial - chemistry</topic><topic>RNA, Bacterial - metabolism</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Transcription, Genetic</topic><topic>Up-Regulation</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Novick, R.P.</creatorcontrib><creatorcontrib>Ross, H.F.</creatorcontrib><creatorcontrib>Projan, S.J.</creatorcontrib><creatorcontrib>Kornblum, J.</creatorcontrib><creatorcontrib>Kreiswirth, B.</creatorcontrib><creatorcontrib>Moghazeh, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Novick, R.P.</au><au>Ross, H.F.</au><au>Projan, S.J.</au><au>Kornblum, J.</au><au>Kreiswirth, B.</au><au>Moghazeh, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1993-10</date><risdate>1993</risdate><volume>12</volume><issue>10</issue><spage>3967</spage><epage>3975</epage><pages>3967-3975</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>The production of most toxins and other exoproteins in Staphylococcus aureus is controlled globally by a complex polycistronic regulatory locus, agr. Secretory proteins are up‐regulated by agr whereas surface proteins are down‐regulated. agr contains two divergent promoters, one of which directs the synthesis of a 514 nucleotide (nt) transcript, RNAIII. In this report, we show that the cloned RNAIII determinant restores both positive and negative regulatory functions of agr to an agr‐null strain and that the RNA itself, rather than any protein, is the effector molecule. RNAIII acts primarily on the initiation of transcription and, secondarily in some cases, at the level of translation. In these cases, translation and transcription are regulated independently. RNAIII probably regulates translation directly by interacting with target gene transcripts and transcription indirectly by means of intermediary protein factors.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>7691599</pmid><doi>10.1002/j.1460-2075.1993.tb06074.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Bacteriology Base Sequence Biological and medical sciences Blotting, Western Down-Regulation Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Microbiology Molecular Sequence Data Nucleic Acid Conformation Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Promoter Regions, Genetic Protein Biosynthesis Restriction Mapping RNA, Bacterial - chemistry RNA, Bacterial - metabolism Staphylococcus aureus - genetics Staphylococcus aureus - pathogenicity Transcription, Genetic Up-Regulation Virulence |
| title | Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule |
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