Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex
To search genetically for additional components of the protein translocation apparatus of mitochondria, we have used low fidelity PCR mutagenesis to generate temperature-sensitive mutants in the outer membrane translocation pore component ISP42. A high copy number suppressor of temperature-sensitive...
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| Veröffentlicht in: | The EMBO journal 1993-08, Vol.12 (8), p.3023-3034 |
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| creator | Kassenbrock, C.K Cao, W Douglas, M.G |
| description | To search genetically for additional components of the protein translocation apparatus of mitochondria, we have used low fidelity PCR mutagenesis to generate temperature-sensitive mutants in the outer membrane translocation pore component ISP42. A high copy number suppressor of temperature-sensitive isp42 has been isolated and sequenced. This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol. Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants. Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p. |
| doi_str_mv | 10.1002/j.1460-2075.1993.tb05971.x |
| format | Article |
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A high copy number suppressor of temperature-sensitive isp42 has been isolated and sequenced. This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol. Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants. Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1993.tb05971.x</identifier><identifier>PMID: 8344244</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Biological and medical sciences ; cell membranes ; Cell structures and functions ; DNA, Fungal ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; genbank/z22815 ; Genes, Suppressor ; Hot Temperature ; Intracellular Membranes - metabolism ; isp6 gene ; Macromolecular Substances ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Membrane Transport Proteins ; mitochondria ; Mitochondria - metabolism ; Mitochondria and cell respiration ; Mitochondrial Membrane Transport Proteins ; Mitochondrial Precursor Protein Import Complex Proteins ; Molecular and cellular biology ; Molecular Sequence Data ; mutagenesis ; mutants ; nucleotide sequences ; Polymerase Chain Reaction ; protein transport ; proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; structural genes</subject><ispartof>The EMBO journal, 1993-08, Vol.12 (8), p.3023-3034</ispartof><rights>1993 European Molecular Biology Organization</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5621-b404b38211958ccfc2d660cc983a0936dc4d7f0ec9ddc895c79d8f0178de8e753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413567/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413567/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27902,27903,53768,53770</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4862057$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8344244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kassenbrock, C.K</creatorcontrib><creatorcontrib>Cao, W</creatorcontrib><creatorcontrib>Douglas, M.G</creatorcontrib><title>Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>To search genetically for additional components of the protein translocation apparatus of mitochondria, we have used low fidelity PCR mutagenesis to generate temperature-sensitive mutants in the outer membrane translocation pore component ISP42. A high copy number suppressor of temperature-sensitive isp42 has been isolated and sequenced. This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol. Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants. Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cell membranes</subject><subject>Cell structures and functions</subject><subject>DNA, Fungal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>genbank/z22815</subject><subject>Genes, Suppressor</subject><subject>Hot Temperature</subject><subject>Intracellular Membranes - metabolism</subject><subject>isp6 gene</subject><subject>Macromolecular Substances</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins</subject><subject>mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria and cell respiration</subject><subject>Mitochondrial Membrane Transport Proteins</subject><subject>Mitochondrial Precursor Protein Import Complex Proteins</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>mutagenesis</subject><subject>mutants</subject><subject>nucleotide sequences</subject><subject>Polymerase Chain Reaction</subject><subject>protein transport</subject><subject>proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>structural genes</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcFu1DAURSMEKkPhExAWQqxIsBM7sZFYlKqUoiKQSteW8-x0PEriwfbQKV_BJ-OQaIAlK1u657537ZtlzwkuCMbl601BaI3zEjesIEJURWwxEw0p9vey1UG6n61wWZOcEi4eZo9C2GCMGW_IUXbEK0pLSlfZz3MzmmgBqVGj1jpYm8GC6hGslVcQjbc_VLRuRK5DF1df6ldIoTCovkeDjQl3o_Y28W6XWDSYofVqNGjrXTR2RCoEB1ZFo9GtjWsU13-0mMjQO5jngxu2vdk_zh50qg_myXIeZ9fvz76efsgvP59fnJ5c5sDqkuQtxbSteEmIYBygg1LXNQYQvFJYVLUGqpsOGxBaAxcMGqF5h0nDteGmYdVx9naeu921g9FgxhSnl1tvB-XvpFNW_quMdi1v3HdJScXqJvlfLn7vvu1MiHKwAUzfp9e7XZCkZo1IixP4ZgbBuxC86Q47CJZTnXIjp87k1Jmc6pRLnXKfzE__TnmwLv0l_cWiq5Ba69KPgg0HjPK6xGwKezJjt7Y3d_8RQJ59evfx9z3NeDbP6JST6sanNddXJSbV9KkMl7T6Bao8y3Q</recordid><startdate>199308</startdate><enddate>199308</enddate><creator>Kassenbrock, C.K</creator><creator>Cao, W</creator><creator>Douglas, M.G</creator><general>Nature Publishing Group</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>199308</creationdate><title>Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex</title><author>Kassenbrock, C.K ; Cao, W ; Douglas, M.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5621-b404b38211958ccfc2d660cc983a0936dc4d7f0ec9ddc895c79d8f0178de8e753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cell membranes</topic><topic>Cell structures and functions</topic><topic>DNA, Fungal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>genbank/z22815</topic><topic>Genes, Suppressor</topic><topic>Hot Temperature</topic><topic>Intracellular Membranes - metabolism</topic><topic>isp6 gene</topic><topic>Macromolecular Substances</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Transport Proteins</topic><topic>mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria and cell respiration</topic><topic>Mitochondrial Membrane Transport Proteins</topic><topic>Mitochondrial Precursor Protein Import Complex Proteins</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>mutagenesis</topic><topic>mutants</topic><topic>nucleotide sequences</topic><topic>Polymerase Chain Reaction</topic><topic>protein transport</topic><topic>proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kassenbrock, C.K</creatorcontrib><creatorcontrib>Cao, W</creatorcontrib><creatorcontrib>Douglas, M.G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kassenbrock, C.K</au><au>Cao, W</au><au>Douglas, M.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1993-08</date><risdate>1993</risdate><volume>12</volume><issue>8</issue><spage>3023</spage><epage>3034</epage><pages>3023-3034</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>To search genetically for additional components of the protein translocation apparatus of mitochondria, we have used low fidelity PCR mutagenesis to generate temperature-sensitive mutants in the outer membrane translocation pore component ISP42. A high copy number suppressor of temperature-sensitive isp42 has been isolated and sequenced. This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol. Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants. Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>8344244</pmid><doi>10.1002/j.1460-2075.1993.tb05971.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences cell membranes Cell structures and functions DNA, Fungal Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism genbank/z22815 Genes, Suppressor Hot Temperature Intracellular Membranes - metabolism isp6 gene Macromolecular Substances Membrane Proteins - genetics Membrane Proteins - metabolism Membrane Transport Proteins mitochondria Mitochondria - metabolism Mitochondria and cell respiration Mitochondrial Membrane Transport Proteins Mitochondrial Precursor Protein Import Complex Proteins Molecular and cellular biology Molecular Sequence Data mutagenesis mutants nucleotide sequences Polymerase Chain Reaction protein transport proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins structural genes |
| title | Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex |
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