Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity
Anemopsis californica has been used empirically to treat infectious diseases. However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) met...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2014-01, Vol.2014 (2014), p.1-8 |
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| creator | Gutiérrez-Lomelí, Melesio Gonzalez-Ávila, Marisela Blasco-Cabal, José Luis Bautista-Bautista, Nereyda Del-Toro-Sánchez, Carmen Lizette Arriaga-Alba, Myriam |
| description | Anemopsis californica has been used empirically to treat infectious diseases. However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) methanolic extracts of A. californica collected in the central Mexican state of Querétaro. Antioxidant properties and total phenols of extracts were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. Mutagenicity was evaluated using the Ames test employing Salmonella enterica serovar Typhimurium strains (TA98, TA100, and TA102), with and without an aroclor 1254 (S9 mixture). Antimutagenesis was performed against mutations induced on the Ames test with MNNG, 2AA, or 4NQO. SME presented the highest antioxidant capacity and total phenolic content. None of the extracts exhibited mutagenicity in the Ames test. The extracts produced a significant reduction in 2AA-induced mutations in S. typhimurium TA98. In both extracts, mutagenesis induced by 4NQO or methyl-N′-nitro-N-nitrosoguanidine (MNNG) was reduced only if the exposure of strains was |
| doi_str_mv | 10.1155/2014/273878 |
| format | Article |
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However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) methanolic extracts of A. californica collected in the central Mexican state of Querétaro. Antioxidant properties and total phenols of extracts were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. Mutagenicity was evaluated using the Ames test employing Salmonella enterica serovar Typhimurium strains (TA98, TA100, and TA102), with and without an aroclor 1254 (S9 mixture). Antimutagenesis was performed against mutations induced on the Ames test with MNNG, 2AA, or 4NQO. SME presented the highest antioxidant capacity and total phenolic content. None of the extracts exhibited mutagenicity in the Ames test. The extracts produced a significant reduction in 2AA-induced mutations in S. typhimurium TA98. In both extracts, mutagenesis induced by 4NQO or methyl-N′-nitro-N-nitrosoguanidine (MNNG) was reduced only if the exposure of strains was <10 μg/Petri dish. A. californca antioxidant properties and its capacity to reduce point mutations render it suitable to enhance medical cancer treatments. The significant effect against antimutagenic 2AA suggests that their consumption would provide protection against carcinogenic polycyclic aromatic compounds.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2014/273878</identifier><identifier>PMID: 25152760</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Ames test ; Anemopsis californica ; Antimutagenicity ; Antioxidants ; Aroclor 1254 ; Aromatic compounds ; Cancer ; Deoxyribonucleic acid ; DNA ; Health aspects ; Infectious diseases ; International organizations ; Medicinal plants ; Medicine, Botanic ; Medicine, Herbal ; Mutagenesis ; Mutagenicity ; Mutation ; PCB ; Pharmacology, Experimental ; Phenolic compounds ; Phenols ; Polychlorinated biphenyls ; Salmonella ; Stevia</subject><ispartof>Evidence-based complementary and alternative medicine, 2014-01, Vol.2014 (2014), p.1-8</ispartof><rights>Copyright © 2014 Carmen Lizette Del-Toro-Sánchez et al.</rights><rights>COPYRIGHT 2014 John Wiley & Sons, Inc.</rights><rights>Copyright © 2014 Carmen Lizette Del-Toro-Sánchez et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2014 Carmen Lizette Del-Toro-Sánchez et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-58693fb2f3875a736a582aa951f0edc7373f1f22789fb6c4e9e4a1f67dcb911b3</citedby><cites>FETCH-LOGICAL-c458t-58693fb2f3875a736a582aa951f0edc7373f1f22789fb6c4e9e4a1f67dcb911b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4135139/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4135139/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25152760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Garrido, Gabino</contributor><contributor>Gabino Garrido</contributor><creatorcontrib>Gutiérrez-Lomelí, Melesio</creatorcontrib><creatorcontrib>Gonzalez-Ávila, Marisela</creatorcontrib><creatorcontrib>Blasco-Cabal, José Luis</creatorcontrib><creatorcontrib>Bautista-Bautista, Nereyda</creatorcontrib><creatorcontrib>Del-Toro-Sánchez, Carmen Lizette</creatorcontrib><creatorcontrib>Arriaga-Alba, Myriam</creatorcontrib><title>Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Anemopsis californica has been used empirically to treat infectious diseases. However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) methanolic extracts of A. californica collected in the central Mexican state of Querétaro. Antioxidant properties and total phenols of extracts were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. Mutagenicity was evaluated using the Ames test employing Salmonella enterica serovar Typhimurium strains (TA98, TA100, and TA102), with and without an aroclor 1254 (S9 mixture). Antimutagenesis was performed against mutations induced on the Ames test with MNNG, 2AA, or 4NQO. SME presented the highest antioxidant capacity and total phenolic content. None of the extracts exhibited mutagenicity in the Ames test. The extracts produced a significant reduction in 2AA-induced mutations in S. typhimurium TA98. In both extracts, mutagenesis induced by 4NQO or methyl-N′-nitro-N-nitrosoguanidine (MNNG) was reduced only if the exposure of strains was <10 μg/Petri dish. A. californca antioxidant properties and its capacity to reduce point mutations render it suitable to enhance medical cancer treatments. The significant effect against antimutagenic 2AA suggests that their consumption would provide protection against carcinogenic polycyclic aromatic compounds.</description><subject>Ames test</subject><subject>Anemopsis californica</subject><subject>Antimutagenicity</subject><subject>Antioxidants</subject><subject>Aroclor 1254</subject><subject>Aromatic compounds</subject><subject>Cancer</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Health aspects</subject><subject>Infectious diseases</subject><subject>International organizations</subject><subject>Medicinal plants</subject><subject>Medicine, Botanic</subject><subject>Medicine, Herbal</subject><subject>Mutagenesis</subject><subject>Mutagenicity</subject><subject>Mutation</subject><subject>PCB</subject><subject>Pharmacology, Experimental</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Polychlorinated biphenyls</subject><subject>Salmonella</subject><subject>Stevia</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkUFrFDEYhoMotlZP3iXgTVmbL5lMkoswlGoLFUEqeAuZbLKbMpOsSba2_94sW9d685RAnu_h_fIi9BrIBwDOTymB7pQKJoV8go5BdLDoqJRPD3fx4wi9KOWGEKqEEM_REeXAqejJMZqGWMO8rWblYrCh3uPk8RdX1yamKVh8flezsbVgn9OMh-jmtCmhYGum4FNuMwaHiL-5ydSQIq4JX69dyHjnTXdhaWLFg63htrlfomfeTMW9ejhP0PdP59dnF4urr58vz4arhe24rAsue8X8SH1biRvBesMlNUZx8MQtrWCCefCUCqn82NvOKdcZ8L1Y2lEBjOwEfdx7N9txbhMutiUmvclhNvleJxP0vy8xrPUq3eoOGAemmuDtgyCnn1tXqr5J2xxbZk1JT2RjFPlLrczkdIg-7f5qDsXqoQMBjVRdo97vKZtTKdn5Qw4geleg3hWo9wU2-s3j6Af2T2MNeLcH1iEuza_wfzbXEOfNIxgkg579BvKErd4</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Gutiérrez-Lomelí, Melesio</creator><creator>Gonzalez-Ávila, Marisela</creator><creator>Blasco-Cabal, José Luis</creator><creator>Bautista-Bautista, Nereyda</creator><creator>Del-Toro-Sánchez, Carmen Lizette</creator><creator>Arriaga-Alba, Myriam</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity</title><author>Gutiérrez-Lomelí, Melesio ; Gonzalez-Ávila, Marisela ; Blasco-Cabal, José Luis ; Bautista-Bautista, Nereyda ; Del-Toro-Sánchez, Carmen Lizette ; Arriaga-Alba, Myriam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-58693fb2f3875a736a582aa951f0edc7373f1f22789fb6c4e9e4a1f67dcb911b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ames test</topic><topic>Anemopsis californica</topic><topic>Antimutagenicity</topic><topic>Antioxidants</topic><topic>Aroclor 1254</topic><topic>Aromatic compounds</topic><topic>Cancer</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Health aspects</topic><topic>Infectious diseases</topic><topic>International organizations</topic><topic>Medicinal plants</topic><topic>Medicine, Botanic</topic><topic>Medicine, Herbal</topic><topic>Mutagenesis</topic><topic>Mutagenicity</topic><topic>Mutation</topic><topic>PCB</topic><topic>Pharmacology, Experimental</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Polychlorinated biphenyls</topic><topic>Salmonella</topic><topic>Stevia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gutiérrez-Lomelí, Melesio</creatorcontrib><creatorcontrib>Gonzalez-Ávila, Marisela</creatorcontrib><creatorcontrib>Blasco-Cabal, José Luis</creatorcontrib><creatorcontrib>Bautista-Bautista, Nereyda</creatorcontrib><creatorcontrib>Del-Toro-Sánchez, Carmen Lizette</creatorcontrib><creatorcontrib>Arriaga-Alba, Myriam</creatorcontrib><collection>الدوريات العلمية والإحصائية - 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However, there are no antimutagenic evaluation reports on this plant. The present study evaluated the antioxidant activity in relation to the mutagenic and antimutagenic activity properties of leaf (LME) and stem (SME) methanolic extracts of A. californica collected in the central Mexican state of Querétaro. Antioxidant properties and total phenols of extracts were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. Mutagenicity was evaluated using the Ames test employing Salmonella enterica serovar Typhimurium strains (TA98, TA100, and TA102), with and without an aroclor 1254 (S9 mixture). Antimutagenesis was performed against mutations induced on the Ames test with MNNG, 2AA, or 4NQO. SME presented the highest antioxidant capacity and total phenolic content. None of the extracts exhibited mutagenicity in the Ames test. The extracts produced a significant reduction in 2AA-induced mutations in S. typhimurium TA98. In both extracts, mutagenesis induced by 4NQO or methyl-N′-nitro-N-nitrosoguanidine (MNNG) was reduced only if the exposure of strains was <10 μg/Petri dish. A. californca antioxidant properties and its capacity to reduce point mutations render it suitable to enhance medical cancer treatments. The significant effect against antimutagenic 2AA suggests that their consumption would provide protection against carcinogenic polycyclic aromatic compounds.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>25152760</pmid><doi>10.1155/2014/273878</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley-Blackwell Open Access Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Ames test Anemopsis californica Antimutagenicity Antioxidants Aroclor 1254 Aromatic compounds Cancer Deoxyribonucleic acid DNA Health aspects Infectious diseases International organizations Medicinal plants Medicine, Botanic Medicine, Herbal Mutagenesis Mutagenicity Mutation PCB Pharmacology, Experimental Phenolic compounds Phenols Polychlorinated biphenyls Salmonella Stevia |
| title | Antimutagenicity of Methanolic Extracts from Anemopsis californica in Relation to Their Antioxidant Activity |
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