Expansion of the CRISPR–Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs

The repurposed CRISPR–Cas9 system has recently emerged as a revolutionary genome-editing tool. Here we report a modification in the expression of the guide RNA (gRNA) required for targeting that greatly expands the targetable genome. gRNA expression through the commonly used U6 promoter requires a g...

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