Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons
Rapid input-restricted change in gene expression is an important aspect of synaptic plasticity requiring complex mechanisms of post-transcriptional mRNA trafficking and regulation. Small non-coding miRNA are uniquely poised to support these functions by providing a nucleic-acid-based specificity com...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 2014-08, Vol.42 (14), p.9195-9208 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9208 |
|---|---|
| container_issue | 14 |
| container_start_page | 9195 |
| container_title | Nucleic acids research |
| container_volume | 42 |
| creator | Goldie, Belinda J Dun, Matthew D Lin, Minjie Smith, Nathan D Verrills, Nicole M Dayas, Christopher V Cairns, Murray J |
| description | Rapid input-restricted change in gene expression is an important aspect of synaptic plasticity requiring complex mechanisms of post-transcriptional mRNA trafficking and regulation. Small non-coding miRNA are uniquely poised to support these functions by providing a nucleic-acid-based specificity component for universal-sequence-dependent RNA binding complexes. We investigated the subcellular distribution of these molecules in resting and potassium chloride depolarized human neuroblasts, and found both selective enrichment and depletion in neurites. Depolarization was associated with a neurite-restricted decrease in miRNA expression; a subset of these molecules was recovered from the depolarization medium in nuclease resistant extracellular exosomes. These vesicles were enriched with primate specific miRNA and the synaptic-plasticity-associated protein MAP1b. These findings further support a role for miRNA as neural plasticity regulators, as they are compartmentalized in neurons and undergo activity-associated redistribution or release into the extracellular matrix. |
| doi_str_mv | 10.1093/nar/gku594 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4132720</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>25053844</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-d5dc3db28bf447d46f157ed179f4626190cd71c45b562b52bd42faad1d7013d63</originalsourceid><addsrcrecordid>eNpVkNtKw0AQhhdRbK3e-ACSayF2D7NJcyOU4gmqguj1stlDuzbJht20WJ_eSLXo1cA3__wDH0LnBF8RXLBxI8N4sVrzAg7QkLCMplBk9BANMcM8JRgmA3QS4zvGBAiHYzSgHHM2ARgiNVWd27hum8oYvXKyMzqp3cvTNJHBJK1UK7nokWuSR9mSMjVNcGrZE_Pho69NTIKpjIw9scHXiTatr2Rwnz1ozDr4Jp6iIyuraM5-5gi93d68zu7T-fPdw2w6TxUAdKnmWjFd0klpAXINmSU8N5rkhYWMZqTASudEAS95RktOSw3USqmJzjFhOmMjdL3rbddlbbQyTRdkJdrgahm2wksn_m8atxQLvxFAGM0p7gsudwUq-BiDsftbgsW3atGrFjvVffji77d99Nct-wIQxn38</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons</title><source>Oxford Journals Open Access Collection</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Goldie, Belinda J ; Dun, Matthew D ; Lin, Minjie ; Smith, Nathan D ; Verrills, Nicole M ; Dayas, Christopher V ; Cairns, Murray J</creator><creatorcontrib>Goldie, Belinda J ; Dun, Matthew D ; Lin, Minjie ; Smith, Nathan D ; Verrills, Nicole M ; Dayas, Christopher V ; Cairns, Murray J</creatorcontrib><description>Rapid input-restricted change in gene expression is an important aspect of synaptic plasticity requiring complex mechanisms of post-transcriptional mRNA trafficking and regulation. Small non-coding miRNA are uniquely poised to support these functions by providing a nucleic-acid-based specificity component for universal-sequence-dependent RNA binding complexes. We investigated the subcellular distribution of these molecules in resting and potassium chloride depolarized human neuroblasts, and found both selective enrichment and depletion in neurites. Depolarization was associated with a neurite-restricted decrease in miRNA expression; a subset of these molecules was recovered from the depolarization medium in nuclease resistant extracellular exosomes. These vesicles were enriched with primate specific miRNA and the synaptic-plasticity-associated protein MAP1b. These findings further support a role for miRNA as neural plasticity regulators, as they are compartmentalized in neurons and undergo activity-associated redistribution or release into the extracellular matrix.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/gku594</identifier><identifier>PMID: 25053844</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Cell Line, Tumor ; Down-Regulation ; Exosomes - chemistry ; Exosomes - metabolism ; Humans ; MicroRNAs - analysis ; MicroRNAs - metabolism ; Microtubule-Associated Proteins - analysis ; Molecular Biology ; Nerve Tissue Proteins - analysis ; Neurites - chemistry ; Neurites - metabolism ; Neurons - metabolism ; Neurons - physiology ; Protein Biosynthesis ; Proteome - chemistry ; RNA, Messenger - metabolism ; Signal Transduction ; Transcription, Genetic</subject><ispartof>Nucleic acids research, 2014-08, Vol.42 (14), p.9195-9208</ispartof><rights>The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.</rights><rights>The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-d5dc3db28bf447d46f157ed179f4626190cd71c45b562b52bd42faad1d7013d63</citedby><cites>FETCH-LOGICAL-c444t-d5dc3db28bf447d46f157ed179f4626190cd71c45b562b52bd42faad1d7013d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132720/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132720/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25053844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goldie, Belinda J</creatorcontrib><creatorcontrib>Dun, Matthew D</creatorcontrib><creatorcontrib>Lin, Minjie</creatorcontrib><creatorcontrib>Smith, Nathan D</creatorcontrib><creatorcontrib>Verrills, Nicole M</creatorcontrib><creatorcontrib>Dayas, Christopher V</creatorcontrib><creatorcontrib>Cairns, Murray J</creatorcontrib><title>Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Rapid input-restricted change in gene expression is an important aspect of synaptic plasticity requiring complex mechanisms of post-transcriptional mRNA trafficking and regulation. Small non-coding miRNA are uniquely poised to support these functions by providing a nucleic-acid-based specificity component for universal-sequence-dependent RNA binding complexes. We investigated the subcellular distribution of these molecules in resting and potassium chloride depolarized human neuroblasts, and found both selective enrichment and depletion in neurites. Depolarization was associated with a neurite-restricted decrease in miRNA expression; a subset of these molecules was recovered from the depolarization medium in nuclease resistant extracellular exosomes. These vesicles were enriched with primate specific miRNA and the synaptic-plasticity-associated protein MAP1b. These findings further support a role for miRNA as neural plasticity regulators, as they are compartmentalized in neurons and undergo activity-associated redistribution or release into the extracellular matrix.</description><subject>Cell Line, Tumor</subject><subject>Down-Regulation</subject><subject>Exosomes - chemistry</subject><subject>Exosomes - metabolism</subject><subject>Humans</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - metabolism</subject><subject>Microtubule-Associated Proteins - analysis</subject><subject>Molecular Biology</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Neurites - chemistry</subject><subject>Neurites - metabolism</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Protein Biosynthesis</subject><subject>Proteome - chemistry</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkNtKw0AQhhdRbK3e-ACSayF2D7NJcyOU4gmqguj1stlDuzbJht20WJ_eSLXo1cA3__wDH0LnBF8RXLBxI8N4sVrzAg7QkLCMplBk9BANMcM8JRgmA3QS4zvGBAiHYzSgHHM2ARgiNVWd27hum8oYvXKyMzqp3cvTNJHBJK1UK7nokWuSR9mSMjVNcGrZE_Pho69NTIKpjIw9scHXiTatr2Rwnz1ozDr4Jp6iIyuraM5-5gi93d68zu7T-fPdw2w6TxUAdKnmWjFd0klpAXINmSU8N5rkhYWMZqTASudEAS95RktOSw3USqmJzjFhOmMjdL3rbddlbbQyTRdkJdrgahm2wksn_m8atxQLvxFAGM0p7gsudwUq-BiDsftbgsW3atGrFjvVffji77d99Nct-wIQxn38</recordid><startdate>20140818</startdate><enddate>20140818</enddate><creator>Goldie, Belinda J</creator><creator>Dun, Matthew D</creator><creator>Lin, Minjie</creator><creator>Smith, Nathan D</creator><creator>Verrills, Nicole M</creator><creator>Dayas, Christopher V</creator><creator>Cairns, Murray J</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20140818</creationdate><title>Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons</title><author>Goldie, Belinda J ; Dun, Matthew D ; Lin, Minjie ; Smith, Nathan D ; Verrills, Nicole M ; Dayas, Christopher V ; Cairns, Murray J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-d5dc3db28bf447d46f157ed179f4626190cd71c45b562b52bd42faad1d7013d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cell Line, Tumor</topic><topic>Down-Regulation</topic><topic>Exosomes - chemistry</topic><topic>Exosomes - metabolism</topic><topic>Humans</topic><topic>MicroRNAs - analysis</topic><topic>MicroRNAs - metabolism</topic><topic>Microtubule-Associated Proteins - analysis</topic><topic>Molecular Biology</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Neurites - chemistry</topic><topic>Neurites - metabolism</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Protein Biosynthesis</topic><topic>Proteome - chemistry</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goldie, Belinda J</creatorcontrib><creatorcontrib>Dun, Matthew D</creatorcontrib><creatorcontrib>Lin, Minjie</creatorcontrib><creatorcontrib>Smith, Nathan D</creatorcontrib><creatorcontrib>Verrills, Nicole M</creatorcontrib><creatorcontrib>Dayas, Christopher V</creatorcontrib><creatorcontrib>Cairns, Murray J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goldie, Belinda J</au><au>Dun, Matthew D</au><au>Lin, Minjie</au><au>Smith, Nathan D</au><au>Verrills, Nicole M</au><au>Dayas, Christopher V</au><au>Cairns, Murray J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>2014-08-18</date><risdate>2014</risdate><volume>42</volume><issue>14</issue><spage>9195</spage><epage>9208</epage><pages>9195-9208</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>Rapid input-restricted change in gene expression is an important aspect of synaptic plasticity requiring complex mechanisms of post-transcriptional mRNA trafficking and regulation. Small non-coding miRNA are uniquely poised to support these functions by providing a nucleic-acid-based specificity component for universal-sequence-dependent RNA binding complexes. We investigated the subcellular distribution of these molecules in resting and potassium chloride depolarized human neuroblasts, and found both selective enrichment and depletion in neurites. Depolarization was associated with a neurite-restricted decrease in miRNA expression; a subset of these molecules was recovered from the depolarization medium in nuclease resistant extracellular exosomes. These vesicles were enriched with primate specific miRNA and the synaptic-plasticity-associated protein MAP1b. These findings further support a role for miRNA as neural plasticity regulators, as they are compartmentalized in neurons and undergo activity-associated redistribution or release into the extracellular matrix.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25053844</pmid><doi>10.1093/nar/gku594</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 2014-08, Vol.42 (14), p.9195-9208 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4132720 |
| source | Oxford Journals Open Access Collection; MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Cell Line, Tumor Down-Regulation Exosomes - chemistry Exosomes - metabolism Humans MicroRNAs - analysis MicroRNAs - metabolism Microtubule-Associated Proteins - analysis Molecular Biology Nerve Tissue Proteins - analysis Neurites - chemistry Neurites - metabolism Neurons - metabolism Neurons - physiology Protein Biosynthesis Proteome - chemistry RNA, Messenger - metabolism Signal Transduction Transcription, Genetic |
| title | Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T15%3A54%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Activity-associated%20miRNA%20are%20packaged%20in%20Map1b-enriched%20exosomes%20released%20from%20depolarized%20neurons&rft.jtitle=Nucleic%20acids%20research&rft.au=Goldie,%20Belinda%20J&rft.date=2014-08-18&rft.volume=42&rft.issue=14&rft.spage=9195&rft.epage=9208&rft.pages=9195-9208&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/gku594&rft_dat=%3Cpubmed_cross%3E25053844%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/25053844&rfr_iscdi=true |