Kinetic and Structural Characterization of Tunnel-Perturbing Mutants in Bradyrhizobium japonicum Proline Utilization A

Proline utilization A from Bradyrhizobium japonicum (BjPutA) is a bifunctional flavoenzyme that catalyzes the oxidation of proline to glutamate using fused proline dehydrogenase (PRODH) and Δ1-pyrroline-5-carboxylate dehydrogenase (P5CDH) domains. Recent crystal structures and kinetic data suggest a...

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Veröffentlicht in:	Biochemistry (Easton) 2014-08, Vol.53 (31), p.5150-5161
Hauptverfasser:	Arentson, Benjamin W, Luo, Min, Pemberton, Travis A, Tanner, John J, Becker, Donald F
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Bradyrhizobium - chemistry Bradyrhizobium - genetics Bradyrhizobium - metabolism Catalytic Domain - genetics Crystallography, X-Ray delta-1-Pyrroline-5-Carboxylate Reductase Kinetics Models, Molecular Mutagenesis, Site-Directed NAD - metabolism Proline - metabolism Proline Oxidase - chemistry Proline Oxidase - genetics Proline Oxidase - metabolism Protein Structure, Quaternary Pyrroline Carboxylate Reductases - chemistry Pyrroline Carboxylate Reductases - genetics Pyrroline Carboxylate Reductases - metabolism Static Electricity
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