Regio-Selective Chemical-Enzymatic Synthesis of Pyrimidine Nucleotides Facilitates RNA Structure and Dynamics Studies

Isotope labeling has revolutionized NMR studies of small nucleic acids, but to extend this technology to larger RNAs, site‐specific labeling tools to expedite NMR structural and dynamics studies are required. Using enzymes from the pentose phosphate pathway, we coupled chemically synthesized uracil...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2014-07, Vol.15 (11), p.1573-1577
Hauptverfasser:	Alvarado, Luigi J., LeBlanc, Regan M., Longhini, Andrew P., Keane, Sarah C., Jain, Niyati, Yildiz, Zehra F., Tolbert, Blanton S., D'Souza, Victoria M., Summers, Michael F., Kreutz, Christoph, Dayie, T. Kwaku
Format:	Artikel
Sprache:	eng
Schlagworte:	dynamics Human immunodeficiency virus 1 Labeling Molecular Dynamics Simulation NMR NMR spectroscopy Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular Pyrimidine Nucleotides - biosynthesis Pyrimidine Nucleotides - chemistry RNA RNA - chemistry RNA - metabolism Stereoisomerism structure
Online-Zugang:	Volltext
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