Click assembly of magnetic nanovectors for gene delivery
Abstract Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the l...
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| Veröffentlicht in: | Biomaterials 2011-04, Vol.32 (10), p.2683-2688 |
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| container_title | Biomaterials |
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| creator | Biswas, Souvik Gordon, Laura E Clark, Geoffrey J Nantz, Michael H |
| description | Abstract Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3 O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials. |
| doi_str_mv | 10.1016/j.biomaterials.2010.12.047 |
| format | Article |
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Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3 O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.12.047</identifier><identifier>PMID: 21255832</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Aminooxy ; blood serum ; breast neoplasms ; Cell Line, Tumor ; Cell Survival ; chemistry ; Click Chemistry - methods ; Dentistry ; DNA - metabolism ; genes ; Humans ; iron oxides ; Lipids - chemistry ; luciferase ; Magnetics ; Magnetofection ; Magnetoplex ; Nanoparticle ; nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Oximation ; Static Electricity ; transfection ; Transfection - methods</subject><ispartof>Biomaterials, 2011-04, Vol.32 (10), p.2683-2688</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. 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All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c672t-96d7971e5190daa058a49609395e056200a769f23854f9e64e9c9a88f1b247533</citedby><cites>FETCH-LOGICAL-c672t-96d7971e5190daa058a49609395e056200a769f23854f9e64e9c9a88f1b247533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961210016200$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21255832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Biswas, Souvik</creatorcontrib><creatorcontrib>Gordon, Laura E</creatorcontrib><creatorcontrib>Clark, Geoffrey J</creatorcontrib><creatorcontrib>Nantz, Michael H</creatorcontrib><title>Click assembly of magnetic nanovectors for gene delivery</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3 O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.</description><subject>Advanced Basic Science</subject><subject>Aminooxy</subject><subject>blood serum</subject><subject>breast neoplasms</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>chemistry</subject><subject>Click Chemistry - methods</subject><subject>Dentistry</subject><subject>DNA - metabolism</subject><subject>genes</subject><subject>Humans</subject><subject>iron oxides</subject><subject>Lipids - chemistry</subject><subject>luciferase</subject><subject>Magnetics</subject><subject>Magnetofection</subject><subject>Magnetoplex</subject><subject>Nanoparticle</subject><subject>nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Oximation</subject><subject>Static Electricity</subject><subject>transfection</subject><subject>Transfection - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksuOEzEQRS0EYkLgF1CLDWwS_H6wGAmFpzQSC2BtOe7q4IzbHuxOpPw9bmUYDSwQK8uqe2-VfQqhFwSvCSby9X69DXl0E5TgYl1TPBfoGnP1AC2IVnolDBYP0QITTldGEnqBntS6x-2OOX2MLiihQmhGF0hvYvDXnasVxm08dXnoRrdLMAXfJZfyEfyUS-2GXLodJOh6iOEI5fQUPRpad3h2ey7R9w_vv20-ra6-fPy8eXu18lLRqXXvlVEEBDG4dw4L7biR2DAjAAtJMXZKmoEyLfhgQHIw3jitB7KlXAnGlujynHtz2I7Qe0hTcdHelDC6crLZBftnJYUfdpePlhPKJVUt4OVtQMk_D1AnO4bqIUaXIB-q1ZIzIynXTfnqn0qiCKaMiTb9Er05S33JtRYY7gYi2M6Q7N7eh2RnSJZQ2yA18_P7T7qz_qbSBO_OAmgfewxQbPUBkoc-lMbD9jn8X5_Lv2J8DCl4F6_hBHWfDyXNHmJrM9iv87rM20Laosxk2C-IfL3m</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Biswas, Souvik</creator><creator>Gordon, Laura E</creator><creator>Clark, Geoffrey J</creator><creator>Nantz, Michael H</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20110401</creationdate><title>Click assembly of magnetic nanovectors for gene delivery</title><author>Biswas, Souvik ; 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| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2011-04, Vol.32 (10), p.2683-2688 |
| issn | 0142-9612 1878-5905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Advanced Basic Science Aminooxy blood serum breast neoplasms Cell Line, Tumor Cell Survival chemistry Click Chemistry - methods Dentistry DNA - metabolism genes Humans iron oxides Lipids - chemistry luciferase Magnetics Magnetofection Magnetoplex Nanoparticle nanoparticles Nanoparticles - chemistry Nanoparticles - ultrastructure Oximation Static Electricity transfection Transfection - methods |
| title | Click assembly of magnetic nanovectors for gene delivery |
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