Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly : In Vitro and In Vivo Analysis
In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), different...
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| Veröffentlicht in: | BioMed research international 2014-01, Vol.2014 (2014), p.1-17 |
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| creator | Fregnan, Federica Varejão, A. S. P. Santos, J. Domingos Bartolo, P. J. Geuna, Stefano Luís, A. L. Mauricio, Ana C. Pereira, T. Amado, Sandra Armada-da-Silva, Paulo A. S. Caseiro, A. R. Gärtner, A. Amorim, Irina Almeida, A. |
| description | In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results in vitro showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed. In vivo testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery. |
| doi_str_mv | 10.1155/2014/302659 |
| format | Article |
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S. P. ; Santos, J. Domingos ; Bartolo, P. J. ; Geuna, Stefano ; Luís, A. L. ; Mauricio, Ana C. ; Pereira, T. ; Amado, Sandra ; Armada-da-Silva, Paulo A. S. ; Caseiro, A. R. ; Gärtner, A. ; Amorim, Irina ; Almeida, A.</creator><contributor>Hande, Manoor Prakash</contributor><creatorcontrib>Fregnan, Federica ; Varejão, A. S. P. ; Santos, J. Domingos ; Bartolo, P. J. ; Geuna, Stefano ; Luís, A. L. ; Mauricio, Ana C. ; Pereira, T. ; Amado, Sandra ; Armada-da-Silva, Paulo A. S. ; Caseiro, A. R. ; Gärtner, A. ; Amorim, Irina ; Almeida, A. ; Hande, Manoor Prakash</creatorcontrib><description>In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results in vitro showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed. In vivo testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/302659</identifier><identifier>PMID: 25121094</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Animals ; Biomechanical Phenomena - drug effects ; Biomedical research ; Cell Differentiation - drug effects ; Colleges & universities ; Disease Models, Animal ; Gene expression ; Humans ; Immunohistochemistry ; Injuries ; Karyotyping ; Membranes ; Membranes, Artificial ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Nerve Regeneration - drug effects ; Nervous system ; Neuroglia - cytology ; Neuroglia - drug effects ; Neurological research ; Peripheral Nerve Injuries - pathology ; Peripheral Nerve Injuries - physiopathology ; Peripheral Nerve Injuries - therapy ; Polyesters - pharmacology ; Rats ; Reaction Time ; Reflex - drug effects ; Regeneration ; Reproducibility of Results ; Sciatic Nerve - drug effects ; Sciatic Nerve - pathology ; Sciatic Nerve - physiopathology ; Stem cells ; Wharton Jelly - cytology</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-17</ispartof><rights>Copyright © 2014 T. Pereira et al.</rights><rights>COPYRIGHT 2014 John Wiley & Sons, Inc.</rights><rights>Copyright © 2014 T. Pereira et al. T. Pereira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 T. Pereira et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-a1f8e435c7025626e68c4b460469e8bdbed00dcb50e8c8ec44b94172be22ce563</citedby><cites>FETCH-LOGICAL-c490t-a1f8e435c7025626e68c4b460469e8bdbed00dcb50e8c8ec44b94172be22ce563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119891/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4119891/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25121094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Hande, Manoor Prakash</contributor><creatorcontrib>Fregnan, Federica</creatorcontrib><creatorcontrib>Varejão, A. S. P.</creatorcontrib><creatorcontrib>Santos, J. Domingos</creatorcontrib><creatorcontrib>Bartolo, P. J.</creatorcontrib><creatorcontrib>Geuna, Stefano</creatorcontrib><creatorcontrib>Luís, A. L.</creatorcontrib><creatorcontrib>Mauricio, Ana C.</creatorcontrib><creatorcontrib>Pereira, T.</creatorcontrib><creatorcontrib>Amado, Sandra</creatorcontrib><creatorcontrib>Armada-da-Silva, Paulo A. S.</creatorcontrib><creatorcontrib>Caseiro, A. R.</creatorcontrib><creatorcontrib>Gärtner, A.</creatorcontrib><creatorcontrib>Amorim, Irina</creatorcontrib><creatorcontrib>Almeida, A.</creatorcontrib><title>Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly : In Vitro and In Vivo Analysis</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>In peripheral nerves MSCs can modulate Wallerian degeneration and the overall regenerative response by acting through paracrine mechanisms directly on regenerating axons or upon the nerve-supporting Schwann cells. In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results in vitro showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed. In vivo testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. 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In the present study, the effect of human MSCs from Wharton’s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-ε-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results in vitro showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed. In vivo testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>25121094</pmid><doi>10.1155/2014/302659</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2014-01, Vol.2014 (2014), p.1-17 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4119891 |
| source | MEDLINE; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Animals Biomechanical Phenomena - drug effects Biomedical research Cell Differentiation - drug effects Colleges & universities Disease Models, Animal Gene expression Humans Immunohistochemistry Injuries Karyotyping Membranes Membranes, Artificial Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Nerve Regeneration - drug effects Nervous system Neuroglia - cytology Neuroglia - drug effects Neurological research Peripheral Nerve Injuries - pathology Peripheral Nerve Injuries - physiopathology Peripheral Nerve Injuries - therapy Polyesters - pharmacology Rats Reaction Time Reflex - drug effects Regeneration Reproducibility of Results Sciatic Nerve - drug effects Sciatic Nerve - pathology Sciatic Nerve - physiopathology Stem cells Wharton Jelly - cytology |
| title | Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-ε-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton’s Jelly : In Vitro and In Vivo Analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T05%3A13%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Promoting%20Nerve%20Regeneration%20in%20a%20Neurotmesis%20Rat%20Model%20Using%20Poly(DL-lactide-%CE%B5-caprolactone)%20Membranes%20and%20Mesenchymal%20Stem%20Cells%20from%20the%20Wharton%E2%80%99s%20Jelly%20:%20In%20Vitro%20and%20In%20Vivo%20Analysis&rft.jtitle=BioMed%20research%20international&rft.au=Fregnan,%20Federica&rft.date=2014-01-01&rft.volume=2014&rft.issue=2014&rft.spage=1&rft.epage=17&rft.pages=1-17&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2014/302659&rft_dat=%3Cgale_pubme%3EA427022495%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1552819792&rft_id=info:pmid/25121094&rft_galeid=A427022495&rfr_iscdi=true |