Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived

In order to advance a culture model of human colonic neoplasia, we developed methods for the isolation and in vitro maintenance of intact colonic crypts from normal human colon tissue and adenomas. Crypts were maintained in three-dimensional Matrigel culture with a simple, serum-free, low Ca2+ (0.15...

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Veröffentlicht in:	Laboratory investigation 2014-02, Vol.94 (2), p.222-234
Hauptverfasser:	Dame, Michael K, Jiang, Yan, Appelman, Henry D, Copley, Kelly D, McClintock, Shannon D, Aslam, Muhammad Nadeem, Attili, Durga, Elmunzer, B Joseph, Brenner, Dean E, Varani, James, Turgeon, D Kim
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1407/651 631/67/1504/1885/1393 692/53 adenoma Adenoma - physiopathology apoptosis Biomarkers - metabolism Cadherins - metabolism Calcium - metabolism chemoprevention Collagen Colon - cytology colon crypt culture Colonic Neoplasms - physiopathology cytokeratin 20 Drug Combinations Fluorescent Antibody Technique Humans Immunohistochemistry Intestinal Mucosa - cytology Keratin-20 - metabolism Ki-67 Antigen - metabolism Laboratory Medicine Laminin Lgr5 Medicine Medicine & Public Health Microscopy, Confocal Models, Biological mucin 2 Mucin-2 - metabolism Pathology Proteoglycans Receptors, G-Protein-Coupled - metabolism research-article Tissue Culture Techniques - methods
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