Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors
Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in sponta...
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| Veröffentlicht in: | Neural regeneration research 2013-02, Vol.8 (4), p.301-312 |
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| creator | Ding, Jun He, Zhili Ruan, Juan Liu, Ying Gong, Chengxin Sun, Shenggang Chen, Honghui |
| description | Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor. |
| doi_str_mv | 10.3969/j.issn.1673-5374.2013.04.002 |
| format | Article |
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This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.</description><identifier>ISSN: 1673-5374</identifier><identifier>EISSN: 1876-7958</identifier><identifier>DOI: 10.3969/j.issn.1673-5374.2013.04.002</identifier><identifier>PMID: 25206670</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Animal experimentation ; Cell differentiation ; Cytokines ; Dehydrogenases ; Fibroblasts ; Growth factors ; Neurogenesis ; Neurons ; Neurotrophic functions ; Physiological aspects ; Proteins ; Stem cells ; Stem Cells and Neural Regeneration ; 内源性 ; 成年大鼠 ; 成纤维细胞生长因子 ; 海马 ; 睫状神经营养因子 ; 神经细胞分化 ; 神经胶质细胞 ; 表皮生长因子</subject><ispartof>Neural regeneration research, 2013-02, Vol.8 (4), p.301-312</ispartof><rights>COPYRIGHT 2013 Medknow Publications and Media Pvt. Ltd.</rights><rights>2013. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>Copyright: © Neural Regeneration Research 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/88507X/88507X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107532/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4107532/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25206670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Jun</creatorcontrib><creatorcontrib>He, Zhili</creatorcontrib><creatorcontrib>Ruan, Juan</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Gong, Chengxin</creatorcontrib><creatorcontrib>Sun, Shenggang</creatorcontrib><creatorcontrib>Chen, Honghui</creatorcontrib><title>Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors</title><title>Neural regeneration research</title><addtitle>Neural Regeneration Research</addtitle><description>Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.</description><subject>Animal experimentation</subject><subject>Cell differentiation</subject><subject>Cytokines</subject><subject>Dehydrogenases</subject><subject>Fibroblasts</subject><subject>Growth factors</subject><subject>Neurogenesis</subject><subject>Neurons</subject><subject>Neurotrophic functions</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Stem cells</subject><subject>Stem Cells and Neural Regeneration</subject><subject>内源性</subject><subject>成年大鼠</subject><subject>成纤维细胞生长因子</subject><subject>海马</subject><subject>睫状神经营养因子</subject><subject>神经细胞分化</subject><subject>神经胶质细胞</subject><subject>表皮生长因子</subject><issn>1673-5374</issn><issn>1876-7958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkk1v1DAQhiMEoqXwF1AQHJDQBn87uSBVFR-VKnGBs-V1xlmvsnZqJ6D21zPLdgtFyAePxs-8nnntqnpDScM71b3fNqGU2FCl-UpyLRpGKG-IaAhhj6pT2mq10p1sH2N8ZE6qZ6VsCZFtx_jT6oRJRpTS5LTKl9GPC0QHdfI1xD4NENNSahfGYPNNHWHJac5p2gRXe-vmlOsUf6ftWPfBe8gQ52DngGnUsP0yznW2c70J05Sc3U0ITnkvHLC6PK-eeDsWeHG3n1XfP338dvFldfX18-XF-dXKCcbnFWe2Y6RVek1BeIuB58CA2taD6FUP1EvNyNqqTjrbOy01lUDXYHuh9Rr4WfXhoDst6x30DrvEls2Uww4HM8kG8_Akho0Z0g8jKNGSMxR4dxD4aaO3cTDbtOSILZvboWxvy83WwN58ItB6pN_eXZfT9QJlNrtQHIyjjYCGGioVFUp2iiD6-h_0XpnxlulWcd3-oQY7ggnR4zNYtxc157zlUhPB9lTzHwpXD7vgUgQfMP-g4OXfttz7cfwTCLw6AG6T4nAdcPAjI0SH34t2_BcprsmF</recordid><startdate>20130205</startdate><enddate>20130205</enddate><creator>Ding, Jun</creator><creator>He, Zhili</creator><creator>Ruan, Juan</creator><creator>Liu, Ying</creator><creator>Gong, Chengxin</creator><creator>Sun, Shenggang</creator><creator>Chen, Honghui</creator><general>Medknow Publications and Media Pvt. 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Ltd</general><general>Mental Health Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China%New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA%Mental Health Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China</general><general>Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China</general><general>Medknow Publications & Media Pvt Ltd</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M2M</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20130205</creationdate><title>Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors</title><author>Ding, Jun ; He, Zhili ; Ruan, Juan ; Liu, Ying ; Gong, Chengxin ; Sun, Shenggang ; Chen, Honghui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-32a920867b1e4fa867f3e2e1a8fe4d6de1f5720ba695cadc75715e1bead477be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal experimentation</topic><topic>Cell differentiation</topic><topic>Cytokines</topic><topic>Dehydrogenases</topic><topic>Fibroblasts</topic><topic>Growth factors</topic><topic>Neurogenesis</topic><topic>Neurons</topic><topic>Neurotrophic functions</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Stem cells</topic><topic>Stem Cells and Neural Regeneration</topic><topic>内源性</topic><topic>成年大鼠</topic><topic>成纤维细胞生长因子</topic><topic>海马</topic><topic>睫状神经营养因子</topic><topic>神经细胞分化</topic><topic>神经胶质细胞</topic><topic>表皮生长因子</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Jun</creatorcontrib><creatorcontrib>He, Zhili</creatorcontrib><creatorcontrib>Ruan, Juan</creatorcontrib><creatorcontrib>Liu, Ying</creatorcontrib><creatorcontrib>Gong, Chengxin</creatorcontrib><creatorcontrib>Sun, Shenggang</creatorcontrib><creatorcontrib>Chen, Honghui</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Proquest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neural regeneration research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Jun</au><au>He, Zhili</au><au>Ruan, Juan</au><au>Liu, Ying</au><au>Gong, Chengxin</au><au>Sun, Shenggang</au><au>Chen, Honghui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors</atitle><jtitle>Neural regeneration research</jtitle><addtitle>Neural Regeneration Research</addtitle><date>2013-02-05</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>301</spage><epage>312</epage><pages>301-312</pages><issn>1673-5374</issn><eissn>1876-7958</eissn><abstract>Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>25206670</pmid><doi>10.3969/j.issn.1673-5374.2013.04.002</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neural regeneration research, 2013-02, Vol.8 (4), p.301-312 |
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| subjects | Animal experimentation Cell differentiation Cytokines Dehydrogenases Fibroblasts Growth factors Neurogenesis Neurons Neurotrophic functions Physiological aspects Proteins Stem cells Stem Cells and Neural Regeneration 内源性 成年大鼠 成纤维细胞生长因子 海马 睫状神经营养因子 神经细胞分化 神经胶质细胞 表皮生长因子 |
| title | Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors |
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