Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity
Abstract Introduction Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we p...
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| Veröffentlicht in: | Placenta (Eastbourne) 2014-02, Vol.35 (2), p.117-124 |
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| creator | Yong, H.E.J Murthi, P Borg, A Kalionis, B Moses, E.K Brennecke, S.P Keogh, R.J |
| description | Abstract Introduction Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1 , INHA , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 . The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods Third trimester decidual tissues were collected from both normotensive ( n = 21) and SPE pregnancies ( n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results The data demonstrate significantly increased decidual mRNA expression levels of ACVR1 , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 in SPE ( p |
| doi_str_mv | 10.1016/j.placenta.2013.11.008 |
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Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1 , INHA , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 . The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods Third trimester decidual tissues were collected from both normotensive ( n = 21) and SPE pregnancies ( n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results The data demonstrate significantly increased decidual mRNA expression levels of ACVR1 , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 in SPE ( p < 0.05). Increased mRNA expression levels of several genes – INHA , INHBB , COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus ( p < 0.05). Conclusion These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2013.11.008</identifier><identifier>PMID: 24331737</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Activin Receptors, Type I - biosynthesis ; Adult ; Clinical severity ; Collagen Type IV - biosynthesis ; Decidua ; Decidua - metabolism ; Female ; Gene expression ; Genetic Predisposition to Disease - genetics ; Humans ; Inhibin-beta Subunits - biosynthesis ; Inhibins - biosynthesis ; Internal Medicine ; Obstetrics and Gynecology ; Pre-eclampsia ; Pre-Eclampsia - genetics ; Pre-Eclampsia - metabolism ; Pregnancy ; Pregnancy Trimester, Third ; RNA, Messenger - metabolism ; Susceptibility genes</subject><ispartof>Placenta (Eastbourne), 2014-02, Vol.35 (2), p.117-124</ispartof><rights>Elsevier Ltd</rights><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><rights>2013 Elsevier Ltd. All rights reserved. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4418-24141a2df1ae1e192dba27b27f753414bd56ec8d69eec907df5cbf076ee6835f3</citedby><cites>FETCH-LOGICAL-c4418-24141a2df1ae1e192dba27b27f753414bd56ec8d69eec907df5cbf076ee6835f3</cites><orcidid>0000-0002-8814-752X ; 0000-0002-4025-5257</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.placenta.2013.11.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24331737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yong, H.E.J</creatorcontrib><creatorcontrib>Murthi, P</creatorcontrib><creatorcontrib>Borg, A</creatorcontrib><creatorcontrib>Kalionis, B</creatorcontrib><creatorcontrib>Moses, E.K</creatorcontrib><creatorcontrib>Brennecke, S.P</creatorcontrib><creatorcontrib>Keogh, R.J</creatorcontrib><title>Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Introduction Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1 , INHA , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 . The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods Third trimester decidual tissues were collected from both normotensive ( n = 21) and SPE pregnancies ( n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results The data demonstrate significantly increased decidual mRNA expression levels of ACVR1 , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 in SPE ( p < 0.05). Increased mRNA expression levels of several genes – INHA , INHBB , COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus ( p < 0.05). Conclusion These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity.</description><subject>Activin Receptors, Type I - biosynthesis</subject><subject>Adult</subject><subject>Clinical severity</subject><subject>Collagen Type IV - biosynthesis</subject><subject>Decidua</subject><subject>Decidua - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Genetic Predisposition to Disease - genetics</subject><subject>Humans</subject><subject>Inhibin-beta Subunits - biosynthesis</subject><subject>Inhibins - biosynthesis</subject><subject>Internal Medicine</subject><subject>Obstetrics and Gynecology</subject><subject>Pre-eclampsia</subject><subject>Pre-Eclampsia - genetics</subject><subject>Pre-Eclampsia - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, Third</subject><subject>RNA, Messenger - metabolism</subject><subject>Susceptibility genes</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstuFDEQRVsIRIbAL0ResunBZXv6sYmIogCRIpB4SOwst12deHA_cHUPzB_w2bg1mQjYsLEXvveWq05l2RnwNXAoXm3XYzAW-8msBQe5BlhzXj3KVrCRIpfAxeNsxUHJXHGuTrJnRFvOea1APM1OhJISSlmusl_XvY1oCB1zaL2bTWDdx_cXDH-OEYn80LOAOwzEhpZZ0zvvzISsS0fskzipcrTBdCN5w2gmi-PkGx_8tGe32CMxE5EZosH6ZHLsh5_umA2-9zb5KYXHpH2ePWlNIHxxf59mX95cfb58l998eHt9eXGTW6WgyoUCBUa4FgwCQi1cY0TZiLItNzK9NW5ToK1cUSPampeu3dim5WWBWFRy08rT7PyQO85Nh24ZYTRBj9F3Ju71YLz--6X3d_p22GkFvBS8TAEv7wPi8H1GmnTnU9MhmB6HmTSougaZhl4naXGQ2jgQRWwfygDXC0a91UeMesGoAXTCmIxnf37ywXbklgSvD4IEBnceoybrsbfofEQ7aTf4_9c4_yfiyOQb7pG2w7zwTf1oEprrT8syLbu09FaJ6qv8DSc-y5E</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Yong, H.E.J</creator><creator>Murthi, P</creator><creator>Borg, A</creator><creator>Kalionis, B</creator><creator>Moses, E.K</creator><creator>Brennecke, S.P</creator><creator>Keogh, R.J</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8814-752X</orcidid><orcidid>https://orcid.org/0000-0002-4025-5257</orcidid></search><sort><creationdate>201402</creationdate><title>Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity</title><author>Yong, H.E.J ; Murthi, P ; Borg, A ; Kalionis, B ; Moses, E.K ; Brennecke, S.P ; Keogh, R.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4418-24141a2df1ae1e192dba27b27f753414bd56ec8d69eec907df5cbf076ee6835f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Activin Receptors, Type I - biosynthesis</topic><topic>Adult</topic><topic>Clinical severity</topic><topic>Collagen Type IV - biosynthesis</topic><topic>Decidua</topic><topic>Decidua - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Genetic Predisposition to Disease - genetics</topic><topic>Humans</topic><topic>Inhibin-beta Subunits - biosynthesis</topic><topic>Inhibins - biosynthesis</topic><topic>Internal Medicine</topic><topic>Obstetrics and Gynecology</topic><topic>Pre-eclampsia</topic><topic>Pre-Eclampsia - genetics</topic><topic>Pre-Eclampsia - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, Third</topic><topic>RNA, Messenger - metabolism</topic><topic>Susceptibility genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yong, H.E.J</creatorcontrib><creatorcontrib>Murthi, P</creatorcontrib><creatorcontrib>Borg, A</creatorcontrib><creatorcontrib>Kalionis, B</creatorcontrib><creatorcontrib>Moses, E.K</creatorcontrib><creatorcontrib>Brennecke, S.P</creatorcontrib><creatorcontrib>Keogh, R.J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yong, H.E.J</au><au>Murthi, P</au><au>Borg, A</au><au>Kalionis, B</au><au>Moses, E.K</au><au>Brennecke, S.P</au><au>Keogh, R.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2014-02</date><risdate>2014</risdate><volume>35</volume><issue>2</issue><spage>117</spage><epage>124</epage><pages>117-124</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><abstract>Abstract Introduction Pre-eclampsia (PE) has a familial association, with daughters of women who had PE during pregnancy having more than twice the risk of developing PE themselves. Through genome-wide linkage and genetic association studies in PE-affected families and large population samples, we previously identified the following as positional candidate maternal susceptibility genes for PE; ACVR1 , INHA , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 . The aims of this study were to determine mRNA expression levels of previously identified candidate maternal pre-eclampsia susceptibility genes from normotensive and severe PE (SPE) pregnancies and correlate mRNA expression levels with the clinical severity of SPE. Methods Third trimester decidual tissues were collected from both normotensive ( n = 21) and SPE pregnancies ( n = 24) and mRNA expression levels were determined by real-time PCR. Gene expression was then correlated with several parameters of clinical severity in SPE. Statistical significance was determined by Mann–Whitney U test and Spearman's Correlation. Results The data demonstrate significantly increased decidual mRNA expression levels of ACVR1 , INHBB , ERAP1 , ERAP2 , LNPEP , COL4A1 and COL4A2 in SPE ( p < 0.05). Increased mRNA expression levels of several genes – INHA , INHBB , COL4A1 and COL4A2 were correlated with earlier onset of PE and earlier delivery of the fetus ( p < 0.05). Conclusion These results suggest altered expression of maternal susceptibility genes may play roles in PE development and the course of disease severity.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>24331737</pmid><doi>10.1016/j.placenta.2013.11.008</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8814-752X</orcidid><orcidid>https://orcid.org/0000-0002-4025-5257</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Activin Receptors, Type I - biosynthesis Adult Clinical severity Collagen Type IV - biosynthesis Decidua Decidua - metabolism Female Gene expression Genetic Predisposition to Disease - genetics Humans Inhibin-beta Subunits - biosynthesis Inhibins - biosynthesis Internal Medicine Obstetrics and Gynecology Pre-eclampsia Pre-Eclampsia - genetics Pre-Eclampsia - metabolism Pregnancy Pregnancy Trimester, Third RNA, Messenger - metabolism Susceptibility genes |
| title | Increased decidual mRNA expression levels of candidate maternal pre-eclampsia susceptibility genes are associated with clinical severity |
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