A G-quadruplex–containing RNA activates fluorescence in a GFP-like fluorophore

Spinach is an RNA aptamer analog of GFP that is widely used for fluorescent labeling of cellular RNAs. Crystal structures of Spinach–fluorophore complexes uncover an unusual G-quadruplex RNA fold that is involved in ligand recognition and tuning of Spinach fluorescence properties. Spinach is an in v...

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Veröffentlicht in:	Nat. Chem. Biol 2014-08, Vol.10 (8), p.686-691
Hauptverfasser:	Huang, Hao, Suslov, Nikolai B, Li, Nan-Sheng, Shelke, Sandip A, Evans, Molly E, Koldobskaya, Yelena, Rice, Phoebe A, Piccirilli, Joseph A
Format:	Artikel
Sprache:	eng
Schlagworte:	14/1 38/71 45/29 631/1647/245 631/337/2179 631/535/1266 631/92/500 82/16 Base Sequence Benzyl Compounds - chemistry Benzyl Compounds - metabolism Binding Sites Biochemical Engineering Biochemistry Bioengineering Bioorganic Chemistry Cell Biology Chemistry Chemistry/Food Science Crystallography Crystallography, X-Ray Fluorescence Fluorescent Dyes - metabolism G-Quadruplexes Green Fluorescent Proteins Hydrogen Bonding Imidazolines - chemistry Imidazolines - metabolism Ligands Molecular Sequence Data Nucleic Acid Conformation Ribonucleic acid RNA RNA - chemistry RNA, Plant - chemistry Spinach Spinacia oleracea - genetics Topology Vegetables
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