Establishment of a Bacterial Expression System and Immunoassay Platform for the Major Capsid Protein of HcRNAV, a Dinoflagellate-Infecting RNA Virus

HcRNAV is a small icosahedral virus that infects the shellfish-killing marine dinoflagellate Heterocapsa circularisquama, which harbors a dicistronic linear single-stranded RNA (ssRNA) genome ca. 4.4 kb in length. Its major capsid protein (MCP) gene sequence is not expressed by various strains of Es...

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Veröffentlicht in:	Microbes and Environments 2012, Vol.27(4), pp.483-489
Hauptverfasser:	Wada, Kei, Kimura, Kei, Hasegawa, Akifumi, Fukuyama, Keiichi, Nagasaki, Keizo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - immunology bacterial expression Capsid Proteins - biosynthesis Capsid Proteins - genetics Capsid Proteins - immunology Codon - genetics Dinoflagellida - virology Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genetic Vectors Heterocapsa circularisquama Heterocapsa circularisquama RNA virus Immunization major capsid protein Microscopy, Fluorescence polyclonal antibody Rabbits Recombinant Proteins - immunology RNA Viruses - genetics RNA Viruses - immunology RNA, Viral - genetics
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