A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma
To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody. Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF si...
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| Veröffentlicht in: | International journal of molecular sciences 2014-05, Vol.15 (6), p.9481-9496 |
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| container_title | International journal of molecular sciences |
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| creator | Wu, Wen Li, Shenghua Zhang, Weijing Sun, Jian Ren, Guangda Dong, Quanchao |
| description | To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.
Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies. |
| doi_str_mv | 10.3390/ijms15069481 |
| format | Article |
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Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms15069481</identifier><identifier>PMID: 24879522</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino Acid Sequence ; Animals ; B-Cell Activating Factor - immunology ; B-Lymphocytes - drug effects ; B-Lymphocytes - immunology ; B-Lymphocytes - pathology ; Camelids, New World ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cloning, Molecular ; E coli ; Escherichia coli ; Escherichia coli - genetics ; Gene expression ; Humans ; Immunoassay ; Immunoglobulin Heavy Chains - chemistry ; Immunoglobulin Heavy Chains - genetics ; Immunoglobulin Heavy Chains - immunology ; Immunoglobulin Heavy Chains - pharmacology ; Lymphoma ; Lymphoma, B-Cell - drug therapy ; Lymphoma, B-Cell - immunology ; Molecular Sequence Data ; Peptide Library ; Sequence Alignment ; Single-Domain Antibodies - chemistry ; Single-Domain Antibodies - genetics ; Single-Domain Antibodies - immunology ; Single-Domain Antibodies - pharmacology</subject><ispartof>International journal of molecular sciences, 2014-05, Vol.15 (6), p.9481-9496</ispartof><rights>Copyright MDPI AG 2014</rights><rights>2014 by the authors; licensee MDPI, Basel, Switzerland. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-cccc4198e5bc182ee82d67aee52f7ed7ffbcea89bbaff814fce5c5562a551e3f3</citedby><cites>FETCH-LOGICAL-c445t-cccc4198e5bc182ee82d67aee52f7ed7ffbcea89bbaff814fce5c5562a551e3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100105/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4100105/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24879522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Wen</creatorcontrib><creatorcontrib>Li, Shenghua</creatorcontrib><creatorcontrib>Zhang, Weijing</creatorcontrib><creatorcontrib>Sun, Jian</creatorcontrib><creatorcontrib>Ren, Guangda</creatorcontrib><creatorcontrib>Dong, Quanchao</creatorcontrib><title>A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.
Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>B-Cell Activating Factor - immunology</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - pathology</subject><subject>Camelids, New World</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cloning, Molecular</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoglobulin Heavy Chains - chemistry</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>Immunoglobulin Heavy Chains - immunology</subject><subject>Immunoglobulin Heavy Chains - pharmacology</subject><subject>Lymphoma</subject><subject>Lymphoma, B-Cell - drug therapy</subject><subject>Lymphoma, B-Cell - immunology</subject><subject>Molecular Sequence Data</subject><subject>Peptide Library</subject><subject>Sequence Alignment</subject><subject>Single-Domain Antibodies - chemistry</subject><subject>Single-Domain Antibodies - genetics</subject><subject>Single-Domain Antibodies - immunology</subject><subject>Single-Domain Antibodies - pharmacology</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkctLAzEQxoMovm-eJeDFg6t57mYvgopaodCLeg3ZdNJu2d3UZFvof2_qi-rFgTBD5sfHzHwInVByyXlJrupZG6kkeSkU3UL7VDCWEZIX2xv1HjqIcUYI40yWu2iPCVWUkrF9NLrBnV9Cg18HA2y6vq78eIV7EybQ190E91PAt9hC02TG9vXSfPy6VPuAXXq32bqJm1U7n_rWHKEdZ5oIx1_5EL083D_fDbLh6PHp7maYWSFkn9kUgpYKZGWpYgCKjfPCAEjmChgXzlUWjCqryjinqHAWpJUyZ0ZKCtzxQ3T9qTtfVC2MLXR9MI2eh7o1YaW9qfXvTldP9cQvtaCEUCKTwPmXQPBvC4i9buu4XsV04BdRU8ULTtIByf-o5LRMw1Ge0LM_6MwvQpcukShBVE5ovqYuPikbfIwB3M_clOi1qXrT1ISfbu76A3-7yN8B4iqeIg</recordid><startdate>20140528</startdate><enddate>20140528</enddate><creator>Wu, Wen</creator><creator>Li, Shenghua</creator><creator>Zhang, Weijing</creator><creator>Sun, Jian</creator><creator>Ren, Guangda</creator><creator>Dong, Quanchao</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20140528</creationdate><title>A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma</title><author>Wu, Wen ; Li, Shenghua ; Zhang, Weijing ; Sun, Jian ; Ren, Guangda ; Dong, Quanchao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-cccc4198e5bc182ee82d67aee52f7ed7ffbcea89bbaff814fce5c5562a551e3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>B-Cell Activating Factor - immunology</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - pathology</topic><topic>Camelids, New World</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cloning, Molecular</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoglobulin Heavy Chains - chemistry</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>Immunoglobulin Heavy Chains - immunology</topic><topic>Immunoglobulin Heavy Chains - pharmacology</topic><topic>Lymphoma</topic><topic>Lymphoma, B-Cell - drug therapy</topic><topic>Lymphoma, B-Cell - immunology</topic><topic>Molecular Sequence Data</topic><topic>Peptide Library</topic><topic>Sequence Alignment</topic><topic>Single-Domain Antibodies - chemistry</topic><topic>Single-Domain Antibodies - genetics</topic><topic>Single-Domain Antibodies - immunology</topic><topic>Single-Domain Antibodies - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Wen</creatorcontrib><creatorcontrib>Li, Shenghua</creatorcontrib><creatorcontrib>Zhang, Weijing</creatorcontrib><creatorcontrib>Sun, Jian</creatorcontrib><creatorcontrib>Ren, Guangda</creatorcontrib><creatorcontrib>Dong, Quanchao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Wen</au><au>Li, Shenghua</au><au>Zhang, Weijing</au><au>Sun, Jian</au><au>Ren, Guangda</au><au>Dong, Quanchao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2014-05-28</date><risdate>2014</risdate><volume>15</volume><issue>6</issue><spage>9481</spage><epage>9496</epage><pages>9481-9496</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>To construct an immune alpaca phage display library, in order to obtain a single domain anti-BAFF (B cell-activating factor) antibody.
Using phage display technology, we constructed an immune alpaca phage display library, selected anti-BAFF single domain antibodies (sdAbs), cloned three anti-BAFF single-domain antibody genes into expression vector pSJF2, and expressed them efficiently in Escherichia coli. The affinity of different anti-BAFF sdAbs were measured by Bio layer interferometry. The in vitro biological function of three sdAbs was investigated by cell counting kit-8 (CCK-8) assay and a competitive enzyme-linked immunosorbent assay (ELISA).
We obtained three anti-BAFF single domain antibodies (anti-BAFF64, anti-BAFF52 and anti-BAFFG3), which were produced in high yield in Escherichia coli and inhibited tumor cell proliferation in vitro.
The selected anti-BAFF antibodies could be candidates for B-cell lymphoma therapies.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>24879522</pmid><doi>10.3390/ijms15069481</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Animals B-Cell Activating Factor - immunology B-Lymphocytes - drug effects B-Lymphocytes - immunology B-Lymphocytes - pathology Camelids, New World Cell Line, Tumor Cell Proliferation - drug effects Cloning, Molecular E coli Escherichia coli Escherichia coli - genetics Gene expression Humans Immunoassay Immunoglobulin Heavy Chains - chemistry Immunoglobulin Heavy Chains - genetics Immunoglobulin Heavy Chains - immunology Immunoglobulin Heavy Chains - pharmacology Lymphoma Lymphoma, B-Cell - drug therapy Lymphoma, B-Cell - immunology Molecular Sequence Data Peptide Library Sequence Alignment Single-Domain Antibodies - chemistry Single-Domain Antibodies - genetics Single-Domain Antibodies - immunology Single-Domain Antibodies - pharmacology |
| title | A novel VHH antibody targeting the B cell-activating factor for B-cell lymphoma |
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