Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile
Background: Human pathogens that can cause infertility may also affect sperm count and quality. Viral infections can be considered as direct and/or indirect cause of male factor infertility. Objective: Our goal was to investigate the prevalence of herpes simplex virus in the semen of infertile men a...
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| Veröffentlicht in: | Iranian journal of reproductive medicine 2014-05, Vol.12 (5), p.327-334 |
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| creator | Amirjannati, Nasser Yaghmaei, Farhad Akhondi, Mohammad Mehdi Nasiri, Mahboubeh Heidari-Vala, Hamed Sehhat, Zahra |
| description | Background: Human pathogens that can cause infertility may also affect
sperm count and quality. Viral infections can be considered as direct
and/or indirect cause of male factor infertility. Objective: Our goal
was to investigate the prevalence of herpes simplex virus in the semen
of infertile men attending the Avicenna Infertility Clinic, and to
compare it with the herpes virus serology results. Materials and
Methods: This cross sectional study was conducted during 2009-2010.
Infertile men participating without any clinical signs of infection
with herpes simplex virus, and no obvious cause for their infertility
were included. Semen and blood samples were used for Polymerase Chain
Reaction (PCR) and serologic testing for these people. Two samples were
collected: one ml semen sample to verify the existence of genital
herpes simplex virus in infertile men, and blood samples of 217
individuals tested for antibodies to herpes simplex virus. Data were
analyzed by SPSS 16. Results: According to the PCR results of semen
samples the prevalence of herpes simplex in semen was 12% and serologic
test showed 3.2% prevalence within blood. Nine to 10% of IgM negative
were PCR positive and only 2-3% of IgM positive were PCR positive.
Between herpes serologic studies with positive controls and negative
controls by using both tests, there was a significant positive
relationship (r=0.718 and p |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4094658</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1546214712</sourcerecordid><originalsourceid>FETCH-LOGICAL-b220t-88bb727f02e6712b63b5ba1035dd2766b88771d479b9ba737ae52a3809caf2a3</originalsourceid><addsrcrecordid>eNpVUNtKxDAQLaLouvoLkkdfKrm0SRdBkMUbrPiy72XSTrfRNKlJV_TvDawrCgNzZubMOcMcZDNOaZVzptRhNmOyorkshDjJTmN8pVQyycVxdsJLKlip1Cx7e_YWm62FQMC1JGLw1m9MQ1oDG-fjlCCMY_DQ9BivydQjGQN-gEXXIPEd6TGMGEk0w2jxkxhHTGv8CFOfVgdMpeswTMbiWXbUgY14_pPn2fr-br18zFcvD0_L21WuOadTXlVaK646ylEqxrUUutTAqCjblispdVUpxdpCLfRCgxIKsOQgKrpooEtgnt3sZMetHrBt0E0BbD0GM0D4qj2Y-v_Emb7e-I-6oItCllUSuPwRCP59i3GqBxMbtBYc-m2sWVlIzop0W6Je_PX6Ndk_OBGudgRtvDUOfxlNMFDvm2FIwQpaSPENs6GL_w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1546214712</pqid></control><display><type>article</type><title>Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>Bioline International</source><source>PubMed Central</source><creator>Amirjannati, Nasser ; Yaghmaei, Farhad ; Akhondi, Mohammad Mehdi ; Nasiri, Mahboubeh ; Heidari-Vala, Hamed ; Sehhat, Zahra</creator><creatorcontrib>Amirjannati, Nasser ; Yaghmaei, Farhad ; Akhondi, Mohammad Mehdi ; Nasiri, Mahboubeh ; Heidari-Vala, Hamed ; Sehhat, Zahra</creatorcontrib><description>Background: Human pathogens that can cause infertility may also affect
sperm count and quality. Viral infections can be considered as direct
and/or indirect cause of male factor infertility. Objective: Our goal
was to investigate the prevalence of herpes simplex virus in the semen
of infertile men attending the Avicenna Infertility Clinic, and to
compare it with the herpes virus serology results. Materials and
Methods: This cross sectional study was conducted during 2009-2010.
Infertile men participating without any clinical signs of infection
with herpes simplex virus, and no obvious cause for their infertility
were included. Semen and blood samples were used for Polymerase Chain
Reaction (PCR) and serologic testing for these people. Two samples were
collected: one ml semen sample to verify the existence of genital
herpes simplex virus in infertile men, and blood samples of 217
individuals tested for antibodies to herpes simplex virus. Data were
analyzed by SPSS 16. Results: According to the PCR results of semen
samples the prevalence of herpes simplex in semen was 12% and serologic
test showed 3.2% prevalence within blood. Nine to 10% of IgM negative
were PCR positive and only 2-3% of IgM positive were PCR positive.
Between herpes serologic studies with positive controls and negative
controls by using both tests, there was a significant positive
relationship (r=0.718 and p<0.001). The relationship between semen
PCR test results and serological survey of herpes patients with a
negative control in both Pearson and Spearman tests was positive and
significant (r=0.229 and p=0.001). Correlation between the PCR results
of semen samples with two positive control subjects and a positive IgM
test was statistically confirmed (r=0.235 and p<0.001). Conclusion:
We recommend that if there is suspicion to herpes simplex as a
microorganism that theoretically could impact semen parameters and
cause infertility it is prudent to use PCR technique on semen sample
rather than ELISA on serum.</description><identifier>ISSN: 1680-6433</identifier><identifier>EISSN: 2008-2177</identifier><identifier>PMID: 25031577</identifier><language>eng</language><publisher>Iran: Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</publisher><subject>Herpes virus ; IgM ; Infertility ; Original ; Polymerase Chain Reaction ; Semen ; Serologic ; Sexually transmitted diseases (STD)</subject><ispartof>Iranian journal of reproductive medicine, 2014-05, Vol.12 (5), p.327-334</ispartof><rights>Copyright 2014 - Iranian Journal of Reproductive Medicine</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094658/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4094658/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25031577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amirjannati, Nasser</creatorcontrib><creatorcontrib>Yaghmaei, Farhad</creatorcontrib><creatorcontrib>Akhondi, Mohammad Mehdi</creatorcontrib><creatorcontrib>Nasiri, Mahboubeh</creatorcontrib><creatorcontrib>Heidari-Vala, Hamed</creatorcontrib><creatorcontrib>Sehhat, Zahra</creatorcontrib><title>Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile</title><title>Iranian journal of reproductive medicine</title><addtitle>Iran J Reprod Med</addtitle><description>Background: Human pathogens that can cause infertility may also affect
sperm count and quality. Viral infections can be considered as direct
and/or indirect cause of male factor infertility. Objective: Our goal
was to investigate the prevalence of herpes simplex virus in the semen
of infertile men attending the Avicenna Infertility Clinic, and to
compare it with the herpes virus serology results. Materials and
Methods: This cross sectional study was conducted during 2009-2010.
Infertile men participating without any clinical signs of infection
with herpes simplex virus, and no obvious cause for their infertility
were included. Semen and blood samples were used for Polymerase Chain
Reaction (PCR) and serologic testing for these people. Two samples were
collected: one ml semen sample to verify the existence of genital
herpes simplex virus in infertile men, and blood samples of 217
individuals tested for antibodies to herpes simplex virus. Data were
analyzed by SPSS 16. Results: According to the PCR results of semen
samples the prevalence of herpes simplex in semen was 12% and serologic
test showed 3.2% prevalence within blood. Nine to 10% of IgM negative
were PCR positive and only 2-3% of IgM positive were PCR positive.
Between herpes serologic studies with positive controls and negative
controls by using both tests, there was a significant positive
relationship (r=0.718 and p<0.001). The relationship between semen
PCR test results and serological survey of herpes patients with a
negative control in both Pearson and Spearman tests was positive and
significant (r=0.229 and p=0.001). Correlation between the PCR results
of semen samples with two positive control subjects and a positive IgM
test was statistically confirmed (r=0.235 and p<0.001). Conclusion:
We recommend that if there is suspicion to herpes simplex as a
microorganism that theoretically could impact semen parameters and
cause infertility it is prudent to use PCR technique on semen sample
rather than ELISA on serum.</description><subject>Herpes virus</subject><subject>IgM</subject><subject>Infertility</subject><subject>Original</subject><subject>Polymerase Chain Reaction</subject><subject>Semen</subject><subject>Serologic</subject><subject>Sexually transmitted diseases (STD)</subject><issn>1680-6433</issn><issn>2008-2177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNpVUNtKxDAQLaLouvoLkkdfKrm0SRdBkMUbrPiy72XSTrfRNKlJV_TvDawrCgNzZubMOcMcZDNOaZVzptRhNmOyorkshDjJTmN8pVQyycVxdsJLKlip1Cx7e_YWm62FQMC1JGLw1m9MQ1oDG-fjlCCMY_DQ9BivydQjGQN-gEXXIPEd6TGMGEk0w2jxkxhHTGv8CFOfVgdMpeswTMbiWXbUgY14_pPn2fr-br18zFcvD0_L21WuOadTXlVaK646ylEqxrUUutTAqCjblispdVUpxdpCLfRCgxIKsOQgKrpooEtgnt3sZMetHrBt0E0BbD0GM0D4qj2Y-v_Emb7e-I-6oItCllUSuPwRCP59i3GqBxMbtBYc-m2sWVlIzop0W6Je_PX6Ndk_OBGudgRtvDUOfxlNMFDvm2FIwQpaSPENs6GL_w</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Amirjannati, Nasser</creator><creator>Yaghmaei, Farhad</creator><creator>Akhondi, Mohammad Mehdi</creator><creator>Nasiri, Mahboubeh</creator><creator>Heidari-Vala, Hamed</creator><creator>Sehhat, Zahra</creator><general>Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</general><general>Research and Clinical Center for Infertility</general><scope>RBI</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile</title><author>Amirjannati, Nasser ; Yaghmaei, Farhad ; Akhondi, Mohammad Mehdi ; Nasiri, Mahboubeh ; Heidari-Vala, Hamed ; Sehhat, Zahra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b220t-88bb727f02e6712b63b5ba1035dd2766b88771d479b9ba737ae52a3809caf2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Herpes virus</topic><topic>IgM</topic><topic>Infertility</topic><topic>Original</topic><topic>Polymerase Chain Reaction</topic><topic>Semen</topic><topic>Serologic</topic><topic>Sexually transmitted diseases (STD)</topic><toplevel>online_resources</toplevel><creatorcontrib>Amirjannati, Nasser</creatorcontrib><creatorcontrib>Yaghmaei, Farhad</creatorcontrib><creatorcontrib>Akhondi, Mohammad Mehdi</creatorcontrib><creatorcontrib>Nasiri, Mahboubeh</creatorcontrib><creatorcontrib>Heidari-Vala, Hamed</creatorcontrib><creatorcontrib>Sehhat, Zahra</creatorcontrib><collection>Bioline International</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Iranian journal of reproductive medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amirjannati, Nasser</au><au>Yaghmaei, Farhad</au><au>Akhondi, Mohammad Mehdi</au><au>Nasiri, Mahboubeh</au><au>Heidari-Vala, Hamed</au><au>Sehhat, Zahra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile</atitle><jtitle>Iranian journal of reproductive medicine</jtitle><addtitle>Iran J Reprod Med</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>12</volume><issue>5</issue><spage>327</spage><epage>334</epage><pages>327-334</pages><issn>1680-6433</issn><eissn>2008-2177</eissn><abstract>Background: Human pathogens that can cause infertility may also affect
sperm count and quality. Viral infections can be considered as direct
and/or indirect cause of male factor infertility. Objective: Our goal
was to investigate the prevalence of herpes simplex virus in the semen
of infertile men attending the Avicenna Infertility Clinic, and to
compare it with the herpes virus serology results. Materials and
Methods: This cross sectional study was conducted during 2009-2010.
Infertile men participating without any clinical signs of infection
with herpes simplex virus, and no obvious cause for their infertility
were included. Semen and blood samples were used for Polymerase Chain
Reaction (PCR) and serologic testing for these people. Two samples were
collected: one ml semen sample to verify the existence of genital
herpes simplex virus in infertile men, and blood samples of 217
individuals tested for antibodies to herpes simplex virus. Data were
analyzed by SPSS 16. Results: According to the PCR results of semen
samples the prevalence of herpes simplex in semen was 12% and serologic
test showed 3.2% prevalence within blood. Nine to 10% of IgM negative
were PCR positive and only 2-3% of IgM positive were PCR positive.
Between herpes serologic studies with positive controls and negative
controls by using both tests, there was a significant positive
relationship (r=0.718 and p<0.001). The relationship between semen
PCR test results and serological survey of herpes patients with a
negative control in both Pearson and Spearman tests was positive and
significant (r=0.229 and p=0.001). Correlation between the PCR results
of semen samples with two positive control subjects and a positive IgM
test was statistically confirmed (r=0.235 and p<0.001). Conclusion:
We recommend that if there is suspicion to herpes simplex as a
microorganism that theoretically could impact semen parameters and
cause infertility it is prudent to use PCR technique on semen sample
rather than ELISA on serum.</abstract><cop>Iran</cop><pub>Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</pub><pmid>25031577</pmid><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1680-6433 |
| ispartof | Iranian journal of reproductive medicine, 2014-05, Vol.12 (5), p.327-334 |
| issn | 1680-6433 2008-2177 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4094658 |
| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; Bioline International; PubMed Central |
| subjects | Herpes virus IgM Infertility Original Polymerase Chain Reaction Semen Serologic Sexually transmitted diseases (STD) |
| title | Molecular and serologic diagnostic approaches; the prevalence of herpes simplex in idiopathic men infertile |
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