Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons
The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tr...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2014-08, Vol.8 (2), p.628-634 |
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| creator | XING, SHUXING LIU, CONG XU, BING CHEN, JIANCHANG YIN, DONGFENG ZHANG, CHUNHAO |
| description | The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri-n-butyl phosphate (TnBP), 1% Triton-X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Triton-X 100, were used on rabbit semitendinosus muscles and flexor digitorum tendons for 24 h to remove cells. Hematoxylin and eosin staining was applied for histological observation, while tension testing was used for biomechanical studies. The effects of the various decellularization methods on the histological structure and biomechanical properties of rabbit tendons were evaluated. A group of fresh tendons treated with phosphate-buffered saline served as controls. The various decellularization methods resulted in different effects on the tendons. All the treatment groups exhibited a decrease in tendon biomechanical properties, but no statistically significant differences were observed among the experimental groups. The extensibility of the 1% TnBP-treated group was found to be greater than that of the other groups; however, the difference was not statistically significant. Histologically, the 1% TnBP + 0.5% SDS treatment was shown to have the least impact on the rabbit tendon structure, with good decellularization and no clear cellular remnants observed. The 1% Triton-X 100 + 0.5% SDS treatment had a pronounced effect on the tendon collagen structure and a number of collagen ruptures were observed. Overall, 1% TnBP + 0.5% SDS was found to be the most effective compared with the other treatments, as this treatment preserved the tendon collagen structure while completely removing the cells. Tendons treated with 1% TnBP + 0.5% SDS were histologically similar to normal tendon tissue and biomechanically similar to the tendons in the control group. |
| doi_str_mv | 10.3892/etm.2014.1742 |
| format | Article |
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In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri-n-butyl phosphate (TnBP), 1% Triton-X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Triton-X 100, were used on rabbit semitendinosus muscles and flexor digitorum tendons for 24 h to remove cells. Hematoxylin and eosin staining was applied for histological observation, while tension testing was used for biomechanical studies. The effects of the various decellularization methods on the histological structure and biomechanical properties of rabbit tendons were evaluated. A group of fresh tendons treated with phosphate-buffered saline served as controls. The various decellularization methods resulted in different effects on the tendons. All the treatment groups exhibited a decrease in tendon biomechanical properties, but no statistically significant differences were observed among the experimental groups. The extensibility of the 1% TnBP-treated group was found to be greater than that of the other groups; however, the difference was not statistically significant. Histologically, the 1% TnBP + 0.5% SDS treatment was shown to have the least impact on the rabbit tendon structure, with good decellularization and no clear cellular remnants observed. The 1% Triton-X 100 + 0.5% SDS treatment had a pronounced effect on the tendon collagen structure and a number of collagen ruptures were observed. Overall, 1% TnBP + 0.5% SDS was found to be the most effective compared with the other treatments, as this treatment preserved the tendon collagen structure while completely removing the cells. Tendons treated with 1% TnBP + 0.5% SDS were histologically similar to normal tendon tissue and biomechanically similar to the tendons in the control group.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2014.1742</identifier><identifier>PMID: 25009631</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>acellular ; Biomechanics ; Biotechnology ; Collagen ; extracellular matrix ; Knee ; Laboratory animals ; Ligaments ; Medical research ; Medicine, Experimental ; Methods ; Physiological aspects ; Rabbits ; Skin & tissue grafts ; tendon ; Tendons ; Tissue engineering</subject><ispartof>Experimental and therapeutic medicine, 2014-08, Vol.8 (2), p.628-634</ispartof><rights>Copyright © 2014, Spandidos Publications</rights><rights>COPYRIGHT 2014 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2014</rights><rights>Copyright © 2014, Spandidos Publications 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-4cf0c17f9d8d2fd5b13fcc119584b8789901f831ba7a6ad8d950fdfc9171a1ac3</citedby><cites>FETCH-LOGICAL-c475t-4cf0c17f9d8d2fd5b13fcc119584b8789901f831ba7a6ad8d950fdfc9171a1ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079434/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079434/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25009631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>XING, SHUXING</creatorcontrib><creatorcontrib>LIU, CONG</creatorcontrib><creatorcontrib>XU, BING</creatorcontrib><creatorcontrib>CHEN, JIANCHANG</creatorcontrib><creatorcontrib>YIN, DONGFENG</creatorcontrib><creatorcontrib>ZHANG, CHUNHAO</creatorcontrib><title>Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri-n-butyl phosphate (TnBP), 1% Triton-X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Triton-X 100, were used on rabbit semitendinosus muscles and flexor digitorum tendons for 24 h to remove cells. Hematoxylin and eosin staining was applied for histological observation, while tension testing was used for biomechanical studies. The effects of the various decellularization methods on the histological structure and biomechanical properties of rabbit tendons were evaluated. A group of fresh tendons treated with phosphate-buffered saline served as controls. The various decellularization methods resulted in different effects on the tendons. All the treatment groups exhibited a decrease in tendon biomechanical properties, but no statistically significant differences were observed among the experimental groups. The extensibility of the 1% TnBP-treated group was found to be greater than that of the other groups; however, the difference was not statistically significant. Histologically, the 1% TnBP + 0.5% SDS treatment was shown to have the least impact on the rabbit tendon structure, with good decellularization and no clear cellular remnants observed. The 1% Triton-X 100 + 0.5% SDS treatment had a pronounced effect on the tendon collagen structure and a number of collagen ruptures were observed. Overall, 1% TnBP + 0.5% SDS was found to be the most effective compared with the other treatments, as this treatment preserved the tendon collagen structure while completely removing the cells. Tendons treated with 1% TnBP + 0.5% SDS were histologically similar to normal tendon tissue and biomechanically similar to the tendons in the control group.</description><subject>acellular</subject><subject>Biomechanics</subject><subject>Biotechnology</subject><subject>Collagen</subject><subject>extracellular matrix</subject><subject>Knee</subject><subject>Laboratory animals</subject><subject>Ligaments</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Methods</subject><subject>Physiological aspects</subject><subject>Rabbits</subject><subject>Skin & tissue grafts</subject><subject>tendon</subject><subject>Tendons</subject><subject>Tissue engineering</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkktv3CAURq2qUROlWWZbWeqi3XjKxdjAplIUpQ8pUjfJGmEeM6Q2uIAjpb--ODOdPtjwOvrgcqiqS0CblnH8weRpgxGQDVCCX1RnQDluAEH38jBGnMFpdZHSAyqt64Gx7lV1ijuEeN_CWfX9xlqjcqqDrR9ldGFJtTbKjOMylulPmV3w9WTyLugC-XrnUg5j2Dolx1p6XQ8uTEbtpH9emWOYTczOPCdGOQwu19l4HXx6XZ1YOSZzcejPq_tPN3fXX5rbb5-_Xl_dNorQLjdEWaSAWq6ZxlZ3A7RWKQDeMTIwyjhHYFkLg6SylwXiHbLaKg4UJEjVnlcf97nzMkxGK-NzlKOYo5tkfBJBOvHvjnc7sQ2PgiDKSUtKwPtDQAw_FpOymFxa30R6Ux5IAMN9jwjreUHf_oc-hCX6Up4A3hYVlHD8h9rK0QjnbSjnqjVUXBFECKGUQKGaPaViSCkae7wyILH6FsW3WH2L1Xfh3_xd55H-bbcA7_ZAmospp0M6MiWpQaxB5Yv0mLW_AIFHtQY</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>XING, SHUXING</creator><creator>LIU, CONG</creator><creator>XU, BING</creator><creator>CHEN, JIANCHANG</creator><creator>YIN, DONGFENG</creator><creator>ZHANG, CHUNHAO</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140801</creationdate><title>Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons</title><author>XING, SHUXING ; LIU, CONG ; XU, BING ; CHEN, JIANCHANG ; YIN, DONGFENG ; ZHANG, CHUNHAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-4cf0c17f9d8d2fd5b13fcc119584b8789901f831ba7a6ad8d950fdfc9171a1ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>acellular</topic><topic>Biomechanics</topic><topic>Biotechnology</topic><topic>Collagen</topic><topic>extracellular matrix</topic><topic>Knee</topic><topic>Laboratory animals</topic><topic>Ligaments</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Methods</topic><topic>Physiological aspects</topic><topic>Rabbits</topic><topic>Skin & tissue grafts</topic><topic>tendon</topic><topic>Tendons</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>XING, SHUXING</creatorcontrib><creatorcontrib>LIU, CONG</creatorcontrib><creatorcontrib>XU, BING</creatorcontrib><creatorcontrib>CHEN, JIANCHANG</creatorcontrib><creatorcontrib>YIN, DONGFENG</creatorcontrib><creatorcontrib>ZHANG, CHUNHAO</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>XING, SHUXING</au><au>LIU, CONG</au><au>XU, BING</au><au>CHEN, JIANCHANG</au><au>YIN, DONGFENG</au><au>ZHANG, CHUNHAO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>8</volume><issue>2</issue><spage>628</spage><epage>634</epage><pages>628-634</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The aim of the present study was to investigate the effects of various decellularization methods on the histological and biomechanical properties of rabbit tendons. In total, six chemical reagents, including 1% t-octyl-phenoxypolyethoxyethanol (Triton-X 100), 0.5% sodium dodecyl sulfate (SDS), 1% tri-n-butyl phosphate (TnBP), 1% Triton-X 100 + 0.5% SDS, 1% TnBP + 0.5% SDS and 1% TnBP + 1% Triton-X 100, were used on rabbit semitendinosus muscles and flexor digitorum tendons for 24 h to remove cells. Hematoxylin and eosin staining was applied for histological observation, while tension testing was used for biomechanical studies. The effects of the various decellularization methods on the histological structure and biomechanical properties of rabbit tendons were evaluated. A group of fresh tendons treated with phosphate-buffered saline served as controls. The various decellularization methods resulted in different effects on the tendons. All the treatment groups exhibited a decrease in tendon biomechanical properties, but no statistically significant differences were observed among the experimental groups. The extensibility of the 1% TnBP-treated group was found to be greater than that of the other groups; however, the difference was not statistically significant. Histologically, the 1% TnBP + 0.5% SDS treatment was shown to have the least impact on the rabbit tendon structure, with good decellularization and no clear cellular remnants observed. The 1% Triton-X 100 + 0.5% SDS treatment had a pronounced effect on the tendon collagen structure and a number of collagen ruptures were observed. Overall, 1% TnBP + 0.5% SDS was found to be the most effective compared with the other treatments, as this treatment preserved the tendon collagen structure while completely removing the cells. Tendons treated with 1% TnBP + 0.5% SDS were histologically similar to normal tendon tissue and biomechanically similar to the tendons in the control group.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>25009631</pmid><doi>10.3892/etm.2014.1742</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | acellular Biomechanics Biotechnology Collagen extracellular matrix Knee Laboratory animals Ligaments Medical research Medicine, Experimental Methods Physiological aspects Rabbits Skin & tissue grafts tendon Tendons Tissue engineering |
| title | Effects of various decellularization methods on histological and biomechanical properties of rabbit tendons |
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