Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD
Context: The discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D]. Objective: We describe a novel liquid chromatography and tandem mass spectrom...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2014-07, Vol.99 (7), p.2567-2574 |
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| creator | Kaufmann, Martin Gallagher, J. Christopher Peacock, Munro Schlingmann, Karl-Peter Konrad, Martin DeLuca, Hector F Sigueiro, Rita Lopez, Borja Mourino, Antonio Maestro, Miguel St-Arnaud, René Finkelstein, Joel S Cooper, Donald P Jones, Glenville |
| description | Context:
The discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D].
Objective:
We describe a novel liquid chromatography and tandem mass spectrometry-based method involving derivatization with DMEQ-TAD {4-[2-(6,7-dimethoxy-4-methyl-3,4-dihydroquinoxalinyl)ethyl]-1,2,4-triazoline-3,5-dione} to simultaneously assay multiple vitamin D metabolites including 25-hydroxyvitamin D (25-OH-D) and 24,25-(OH)2D using 100 μL of serum with a 5-minute run time.
Design:
The assay uses a newly synthesized internal standard d6-24,25-(OH)2D3 enabling the quantitation of 24,25-(OH)2D3 as well as the determination of the ratio of 25-OH-D3 to 24,25-(OH)2D3, a physiologically useful parameter.
Setting:
We report data on more than 1000 normal and disease samples involving vitamin D deficiency or hypercalcemia in addition to studies involving knockout mouse models.
Results:
The assay showed good correlation with samples from quality assurance schemes for 25-OH-D (25-OH-D2 and 25-OH-D3) determination (−2% to −5% bias) and exhibited low inter- and intraassay coefficients of variation (4%–7%) and lower limits of quantitation of 0.25–0.45 nmol/L. In clinical studies, we found a strong correlation between serum levels of 25-OH-D3 and 24,25-(OH)2D3 (r2 = 0.80) in subjects over a broad range of 25-OH-D3 values and a marked lack of production of 24,25-(OH)2D3 below 25 nmol/L of 25-OH-D. The ratio of 25-OH-D3 to 24,25-(OH)2D3, which remained less than 25 in vitamin D-sufficient subjects (serum 25-OH-D < 50 nmol/L) but was greatly elevated (80–100) in patients with idiopathic infantile hypercalcemia.
Conclusions:
The new method showed good utility in clinical settings involving vitamin D deficiency; supplementation with vitamin D and idiopathic infantile hypercalcemia, as well as in animal models with ablation of selected cytochrome P450-containing enzymes involved in vitamin D metabolism. |
| doi_str_mv | 10.1210/jc.2013-4388 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4079315</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1544322239</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4734-c7603717401ff71e217c5a16ec01229518540da8ee3b5ed699ee747cde8e98bc3</originalsourceid><addsrcrecordid>eNptkc1uEzEUhUcIRENhxxp5yaLT-m_imQ1SlSm0UiJUpRXsLMdz0zg4dmp7UsJz8MA4mlIBwpJl2ee7x1f3FMVbgk8JJfhsrU8pJqzkrK6fFSPS8KoUpBHPixHGlJSNoF-PilcxrjEmnFfsZXFE-VhgXPNR8XNijTNaWXSbjDVpj_wSzc2mt0k58H1E171yySSVjHcHkVbl5b4L_vt-l183xqEWKdchyk-y1JrVv-Jij6aTcjY_m83Rldt5uzPuDrUQzC6b_hiMv5i0Qu3s4rq8OW9fFy-WykZ483geF7cfL24ml-X086eryfm01FwwXmoxxkwQwTFZLgUBSoSuFBmDxoTSpiJ1xXGnagC2qKAbNw2A4EJ3UENTLzQ7Lj4Mvtt-sYFOg0tBWbkNZqPCXnpl5N-KMyt553eSY9EwUmWD948Gwd_3EJPcmKjB2mF2klScM0opazJ6MqA6-BgDLJ--IVgegpRrLQ9BykOQGX_3Z2tP8O_kMsAH4MHbBCF-s_0DBLkCZdNK4rwyWZfZMTebb2Xe7FDGhjJwndfBONgGiFGufR9cnvX_u_kFZkm6jw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1544322239</pqid></control><display><type>article</type><title>Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Journals@Ovid Ovid Autoload</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Kaufmann, Martin ; Gallagher, J. Christopher ; Peacock, Munro ; Schlingmann, Karl-Peter ; Konrad, Martin ; DeLuca, Hector F ; Sigueiro, Rita ; Lopez, Borja ; Mourino, Antonio ; Maestro, Miguel ; St-Arnaud, René ; Finkelstein, Joel S ; Cooper, Donald P ; Jones, Glenville</creator><creatorcontrib>Kaufmann, Martin ; Gallagher, J. Christopher ; Peacock, Munro ; Schlingmann, Karl-Peter ; Konrad, Martin ; DeLuca, Hector F ; Sigueiro, Rita ; Lopez, Borja ; Mourino, Antonio ; Maestro, Miguel ; St-Arnaud, René ; Finkelstein, Joel S ; Cooper, Donald P ; Jones, Glenville</creatorcontrib><description>Context:
The discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D].
Objective:
We describe a novel liquid chromatography and tandem mass spectrometry-based method involving derivatization with DMEQ-TAD {4-[2-(6,7-dimethoxy-4-methyl-3,4-dihydroquinoxalinyl)ethyl]-1,2,4-triazoline-3,5-dione} to simultaneously assay multiple vitamin D metabolites including 25-hydroxyvitamin D (25-OH-D) and 24,25-(OH)2D using 100 μL of serum with a 5-minute run time.
Design:
The assay uses a newly synthesized internal standard d6-24,25-(OH)2D3 enabling the quantitation of 24,25-(OH)2D3 as well as the determination of the ratio of 25-OH-D3 to 24,25-(OH)2D3, a physiologically useful parameter.
Setting:
We report data on more than 1000 normal and disease samples involving vitamin D deficiency or hypercalcemia in addition to studies involving knockout mouse models.
Results:
The assay showed good correlation with samples from quality assurance schemes for 25-OH-D (25-OH-D2 and 25-OH-D3) determination (−2% to −5% bias) and exhibited low inter- and intraassay coefficients of variation (4%–7%) and lower limits of quantitation of 0.25–0.45 nmol/L. In clinical studies, we found a strong correlation between serum levels of 25-OH-D3 and 24,25-(OH)2D3 (r2 = 0.80) in subjects over a broad range of 25-OH-D3 values and a marked lack of production of 24,25-(OH)2D3 below 25 nmol/L of 25-OH-D. The ratio of 25-OH-D3 to 24,25-(OH)2D3, which remained less than 25 in vitamin D-sufficient subjects (serum 25-OH-D < 50 nmol/L) but was greatly elevated (80–100) in patients with idiopathic infantile hypercalcemia.
Conclusions:
The new method showed good utility in clinical settings involving vitamin D deficiency; supplementation with vitamin D and idiopathic infantile hypercalcemia, as well as in animal models with ablation of selected cytochrome P450-containing enzymes involved in vitamin D metabolism.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2013-4388</identifier><identifier>PMID: 24670084</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>24,25-Dihydroxyvitamin D 3 - analysis ; 24,25-Dihydroxyvitamin D 3 - blood ; Animals ; Blood Chemical Analysis - instrumentation ; Blood Chemical Analysis - methods ; Chromatography, Liquid - methods ; Dietary Supplements ; Endocrine Research ; Female ; Humans ; Hypercalcemia - blood ; Hypercalcemia - diagnosis ; Mice ; Mice, Knockout ; Predictive Value of Tests ; Quinoxalines - chemistry ; Tandem Mass Spectrometry - methods ; Triazoles - chemistry ; Vitamin D - administration & dosage ; Vitamin D - analogs & derivatives ; Vitamin D - analysis ; Vitamin D - blood ; Vitamin D Deficiency - blood ; Vitamin D Deficiency - diagnosis</subject><ispartof>The journal of clinical endocrinology and metabolism, 2014-07, Vol.99 (7), p.2567-2574</ispartof><rights>Copyright © 2014 by the Endocrine Society</rights><rights>Copyright © 2014 by The Endocrine Society</rights><rights>Copyright © 2014 by the Endocrine Society 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4734-c7603717401ff71e217c5a16ec01229518540da8ee3b5ed699ee747cde8e98bc3</citedby><cites>FETCH-LOGICAL-c4734-c7603717401ff71e217c5a16ec01229518540da8ee3b5ed699ee747cde8e98bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24670084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaufmann, Martin</creatorcontrib><creatorcontrib>Gallagher, J. Christopher</creatorcontrib><creatorcontrib>Peacock, Munro</creatorcontrib><creatorcontrib>Schlingmann, Karl-Peter</creatorcontrib><creatorcontrib>Konrad, Martin</creatorcontrib><creatorcontrib>DeLuca, Hector F</creatorcontrib><creatorcontrib>Sigueiro, Rita</creatorcontrib><creatorcontrib>Lopez, Borja</creatorcontrib><creatorcontrib>Mourino, Antonio</creatorcontrib><creatorcontrib>Maestro, Miguel</creatorcontrib><creatorcontrib>St-Arnaud, René</creatorcontrib><creatorcontrib>Finkelstein, Joel S</creatorcontrib><creatorcontrib>Cooper, Donald P</creatorcontrib><creatorcontrib>Jones, Glenville</creatorcontrib><title>Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Context:
The discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D].
Objective:
We describe a novel liquid chromatography and tandem mass spectrometry-based method involving derivatization with DMEQ-TAD {4-[2-(6,7-dimethoxy-4-methyl-3,4-dihydroquinoxalinyl)ethyl]-1,2,4-triazoline-3,5-dione} to simultaneously assay multiple vitamin D metabolites including 25-hydroxyvitamin D (25-OH-D) and 24,25-(OH)2D using 100 μL of serum with a 5-minute run time.
Design:
The assay uses a newly synthesized internal standard d6-24,25-(OH)2D3 enabling the quantitation of 24,25-(OH)2D3 as well as the determination of the ratio of 25-OH-D3 to 24,25-(OH)2D3, a physiologically useful parameter.
Setting:
We report data on more than 1000 normal and disease samples involving vitamin D deficiency or hypercalcemia in addition to studies involving knockout mouse models.
Results:
The assay showed good correlation with samples from quality assurance schemes for 25-OH-D (25-OH-D2 and 25-OH-D3) determination (−2% to −5% bias) and exhibited low inter- and intraassay coefficients of variation (4%–7%) and lower limits of quantitation of 0.25–0.45 nmol/L. In clinical studies, we found a strong correlation between serum levels of 25-OH-D3 and 24,25-(OH)2D3 (r2 = 0.80) in subjects over a broad range of 25-OH-D3 values and a marked lack of production of 24,25-(OH)2D3 below 25 nmol/L of 25-OH-D. The ratio of 25-OH-D3 to 24,25-(OH)2D3, which remained less than 25 in vitamin D-sufficient subjects (serum 25-OH-D < 50 nmol/L) but was greatly elevated (80–100) in patients with idiopathic infantile hypercalcemia.
Conclusions:
The new method showed good utility in clinical settings involving vitamin D deficiency; supplementation with vitamin D and idiopathic infantile hypercalcemia, as well as in animal models with ablation of selected cytochrome P450-containing enzymes involved in vitamin D metabolism.</description><subject>24,25-Dihydroxyvitamin D 3 - analysis</subject><subject>24,25-Dihydroxyvitamin D 3 - blood</subject><subject>Animals</subject><subject>Blood Chemical Analysis - instrumentation</subject><subject>Blood Chemical Analysis - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Dietary Supplements</subject><subject>Endocrine Research</subject><subject>Female</subject><subject>Humans</subject><subject>Hypercalcemia - blood</subject><subject>Hypercalcemia - diagnosis</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Predictive Value of Tests</subject><subject>Quinoxalines - chemistry</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Triazoles - chemistry</subject><subject>Vitamin D - administration & dosage</subject><subject>Vitamin D - analogs & derivatives</subject><subject>Vitamin D - analysis</subject><subject>Vitamin D - blood</subject><subject>Vitamin D Deficiency - blood</subject><subject>Vitamin D Deficiency - diagnosis</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc1uEzEUhUcIRENhxxp5yaLT-m_imQ1SlSm0UiJUpRXsLMdz0zg4dmp7UsJz8MA4mlIBwpJl2ee7x1f3FMVbgk8JJfhsrU8pJqzkrK6fFSPS8KoUpBHPixHGlJSNoF-PilcxrjEmnFfsZXFE-VhgXPNR8XNijTNaWXSbjDVpj_wSzc2mt0k58H1E171yySSVjHcHkVbl5b4L_vt-l183xqEWKdchyk-y1JrVv-Jij6aTcjY_m83Rldt5uzPuDrUQzC6b_hiMv5i0Qu3s4rq8OW9fFy-WykZ483geF7cfL24ml-X086eryfm01FwwXmoxxkwQwTFZLgUBSoSuFBmDxoTSpiJ1xXGnagC2qKAbNw2A4EJ3UENTLzQ7Lj4Mvtt-sYFOg0tBWbkNZqPCXnpl5N-KMyt553eSY9EwUmWD948Gwd_3EJPcmKjB2mF2klScM0opazJ6MqA6-BgDLJ--IVgegpRrLQ9BykOQGX_3Z2tP8O_kMsAH4MHbBCF-s_0DBLkCZdNK4rwyWZfZMTebb2Xe7FDGhjJwndfBONgGiFGufR9cnvX_u_kFZkm6jw</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>Kaufmann, Martin</creator><creator>Gallagher, J. Christopher</creator><creator>Peacock, Munro</creator><creator>Schlingmann, Karl-Peter</creator><creator>Konrad, Martin</creator><creator>DeLuca, Hector F</creator><creator>Sigueiro, Rita</creator><creator>Lopez, Borja</creator><creator>Mourino, Antonio</creator><creator>Maestro, Miguel</creator><creator>St-Arnaud, René</creator><creator>Finkelstein, Joel S</creator><creator>Cooper, Donald P</creator><creator>Jones, Glenville</creator><general>Endocrine Society</general><general>Copyright by The Endocrine Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201407</creationdate><title>Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD</title><author>Kaufmann, Martin ; Gallagher, J. Christopher ; Peacock, Munro ; Schlingmann, Karl-Peter ; Konrad, Martin ; DeLuca, Hector F ; Sigueiro, Rita ; Lopez, Borja ; Mourino, Antonio ; Maestro, Miguel ; St-Arnaud, René ; Finkelstein, Joel S ; Cooper, Donald P ; Jones, Glenville</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4734-c7603717401ff71e217c5a16ec01229518540da8ee3b5ed699ee747cde8e98bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>24,25-Dihydroxyvitamin D 3 - analysis</topic><topic>24,25-Dihydroxyvitamin D 3 - blood</topic><topic>Animals</topic><topic>Blood Chemical Analysis - instrumentation</topic><topic>Blood Chemical Analysis - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Dietary Supplements</topic><topic>Endocrine Research</topic><topic>Female</topic><topic>Humans</topic><topic>Hypercalcemia - blood</topic><topic>Hypercalcemia - diagnosis</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Predictive Value of Tests</topic><topic>Quinoxalines - chemistry</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Triazoles - chemistry</topic><topic>Vitamin D - administration & dosage</topic><topic>Vitamin D - analogs & derivatives</topic><topic>Vitamin D - analysis</topic><topic>Vitamin D - blood</topic><topic>Vitamin D Deficiency - blood</topic><topic>Vitamin D Deficiency - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaufmann, Martin</creatorcontrib><creatorcontrib>Gallagher, J. Christopher</creatorcontrib><creatorcontrib>Peacock, Munro</creatorcontrib><creatorcontrib>Schlingmann, Karl-Peter</creatorcontrib><creatorcontrib>Konrad, Martin</creatorcontrib><creatorcontrib>DeLuca, Hector F</creatorcontrib><creatorcontrib>Sigueiro, Rita</creatorcontrib><creatorcontrib>Lopez, Borja</creatorcontrib><creatorcontrib>Mourino, Antonio</creatorcontrib><creatorcontrib>Maestro, Miguel</creatorcontrib><creatorcontrib>St-Arnaud, René</creatorcontrib><creatorcontrib>Finkelstein, Joel S</creatorcontrib><creatorcontrib>Cooper, Donald P</creatorcontrib><creatorcontrib>Jones, Glenville</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaufmann, Martin</au><au>Gallagher, J. Christopher</au><au>Peacock, Munro</au><au>Schlingmann, Karl-Peter</au><au>Konrad, Martin</au><au>DeLuca, Hector F</au><au>Sigueiro, Rita</au><au>Lopez, Borja</au><au>Mourino, Antonio</au><au>Maestro, Miguel</au><au>St-Arnaud, René</au><au>Finkelstein, Joel S</au><au>Cooper, Donald P</au><au>Jones, Glenville</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2014-07</date><risdate>2014</risdate><volume>99</volume><issue>7</issue><spage>2567</spage><epage>2574</epage><pages>2567-2574</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><abstract>Context:
The discovery of hypercalcemic diseases due to loss-of-function mutations in 25-hydroxyvitamin D-24-hydroxylase has placed a new demand for sensitive and precise assays for 24,25-dihydroxyvitamin D [24,25-(OH)2D].
Objective:
We describe a novel liquid chromatography and tandem mass spectrometry-based method involving derivatization with DMEQ-TAD {4-[2-(6,7-dimethoxy-4-methyl-3,4-dihydroquinoxalinyl)ethyl]-1,2,4-triazoline-3,5-dione} to simultaneously assay multiple vitamin D metabolites including 25-hydroxyvitamin D (25-OH-D) and 24,25-(OH)2D using 100 μL of serum with a 5-minute run time.
Design:
The assay uses a newly synthesized internal standard d6-24,25-(OH)2D3 enabling the quantitation of 24,25-(OH)2D3 as well as the determination of the ratio of 25-OH-D3 to 24,25-(OH)2D3, a physiologically useful parameter.
Setting:
We report data on more than 1000 normal and disease samples involving vitamin D deficiency or hypercalcemia in addition to studies involving knockout mouse models.
Results:
The assay showed good correlation with samples from quality assurance schemes for 25-OH-D (25-OH-D2 and 25-OH-D3) determination (−2% to −5% bias) and exhibited low inter- and intraassay coefficients of variation (4%–7%) and lower limits of quantitation of 0.25–0.45 nmol/L. In clinical studies, we found a strong correlation between serum levels of 25-OH-D3 and 24,25-(OH)2D3 (r2 = 0.80) in subjects over a broad range of 25-OH-D3 values and a marked lack of production of 24,25-(OH)2D3 below 25 nmol/L of 25-OH-D. The ratio of 25-OH-D3 to 24,25-(OH)2D3, which remained less than 25 in vitamin D-sufficient subjects (serum 25-OH-D < 50 nmol/L) but was greatly elevated (80–100) in patients with idiopathic infantile hypercalcemia.
Conclusions:
The new method showed good utility in clinical settings involving vitamin D deficiency; supplementation with vitamin D and idiopathic infantile hypercalcemia, as well as in animal models with ablation of selected cytochrome P450-containing enzymes involved in vitamin D metabolism.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>24670084</pmid><doi>10.1210/jc.2013-4388</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-972X |
| ispartof | The journal of clinical endocrinology and metabolism, 2014-07, Vol.99 (7), p.2567-2574 |
| issn | 0021-972X 1945-7197 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4079315 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Ovid Autoload; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | 24,25-Dihydroxyvitamin D 3 - analysis 24,25-Dihydroxyvitamin D 3 - blood Animals Blood Chemical Analysis - instrumentation Blood Chemical Analysis - methods Chromatography, Liquid - methods Dietary Supplements Endocrine Research Female Humans Hypercalcemia - blood Hypercalcemia - diagnosis Mice Mice, Knockout Predictive Value of Tests Quinoxalines - chemistry Tandem Mass Spectrometry - methods Triazoles - chemistry Vitamin D - administration & dosage Vitamin D - analogs & derivatives Vitamin D - analysis Vitamin D - blood Vitamin D Deficiency - blood Vitamin D Deficiency - diagnosis |
| title | Clinical Utility of Simultaneous Quantitation of 25-Hydroxyvitamin D and 24,25-Dihydroxyvitamin D by LC-MS/MS Involving Derivatization With DMEQ-TAD |
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