A macrophage and theca cell-enriched stromal cell population influences growth and survival of immature murine follicles in vitro

Innovations in in vitro ovarian follicle culture have revolutionized the field of fertility preservation, but the successful culturing of isolated primary and small secondary follicles remains difficult. Herein, we describe a revised 3D culture system that uses a feeder layer of ovarian stromal cell...

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Veröffentlicht in:	Reproduction (Cambridge, England) England), 2011-06, Vol.141 (6), p.809-820
Hauptverfasser:	Tingen, Candace M, Kiesewetter, Sarah E, Jozefik, Jennifer, Thomas, Cristina, Tagler, David, Shea, Lonnie, Woodruff, Teresa K
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance androgens Androgens - metabolism Androstenedione - metabolism Animals cell free system Cell Survival Cells, Cultured coculture Coculture Techniques Culture Media - metabolism cytokines Cytokines - metabolism Female Follicle Stimulating Hormone - metabolism macrophages Macrophages - metabolism Macrophages - physiology Mice Ovarian Follicle - metabolism Ovarian Follicle - physiology ovarian follicles Paracrine Communication Phenotype stromal cells Stromal Cells - metabolism Stromal Cells - physiology Theca Cells - metabolism Theca Cells - physiology thecal cells Time Factors
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