Characterization of green fluorescent proteins by 193 nm ultraviolet photodissociation mass spectrometry

We investigate the utility of 193 nm ultraviolet photodissociation (UVPD) in comparison to CID, higher energy CID (HCD), and electron transfer dissociation (ETD) for top down fragmentation of highly homologous green fluorescent proteins (GFP) in the gas phase. Several GFP variants were constructed v...

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Veröffentlicht in:	Proteomics (Weinheim) 2014-05, Vol.14 (10), p.1165-1173
Hauptverfasser:	Cannon, Joe R., Kluwe, Christien, Ellington, Andrew, Brodbelt, Jennifer S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Green fluorescent protein Green Fluorescent Proteins - analysis Green Fluorescent Proteins - chemistry Orbitrap Peptide Fragments - analysis Peptide Fragments - chemistry Proteomics - methods Tandem Mass Spectrometry - methods Technology Top-down Ultraviolet photodissociation Ultraviolet Rays
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