Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients
AIM： To evaluate the diagnostic potential of cytokeratin-19 （CK-19） mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreati...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2007-01, Vol.13 (2), p.257-263 |
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| creator | Hoffmann, Katrin Kerner, Christiane Wilfert, Wolfgang Mueller, Marc Thiery, Joachim Hauss, Johann Witzigmann, Helmut |
| description | AIM： To evaluate the diagnostic potential of cytokeratin-19 （CK-19） mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers. |
| doi_str_mv | 10.3748/wjg.v13.i2.257 |
| format | Article |
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METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v13.i2.257</identifier><identifier>PMID: 17226905</identifier><language>eng</language><publisher>United States: Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Germany%Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Germany</publisher><subject>Biomarkers, Tumor - analysis ; Biomarkers, Tumor - blood ; Bone Marrow - pathology ; Carcinoma, Pancreatic Ductal - diagnosis ; Carcinoma, Pancreatic Ductal - pathology ; Clinical Research ; Humans ; Keratin-19 - genetics ; Neoplastic Cells, Circulating - chemistry ; Neoplastic Cells, Circulating - pathology ; Pancreatic Neoplasms - diagnosis ; Pancreatic Neoplasms - pathology ; Peritoneal Lavage ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - analysis ; RNA, Messenger - blood ; RNA, Neoplasm - analysis ; RNA, Neoplasm - blood ; 细胞角蛋白 ; 胰腺炎 ; 腹腔灌洗</subject><ispartof>World journal of gastroenterology : WJG, 2007-01, Vol.13 (2), p.257-263</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2007 Baishideng Publishing Group Co., Limited. All rights reserved. 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3591-6d18ed9a9fa807d9df6818b6189db0d6d286edf2f009d1c61cdf08800bda4ae63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065954/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065954/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17226905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoffmann, Katrin</creatorcontrib><creatorcontrib>Kerner, Christiane</creatorcontrib><creatorcontrib>Wilfert, Wolfgang</creatorcontrib><creatorcontrib>Mueller, Marc</creatorcontrib><creatorcontrib>Thiery, Joachim</creatorcontrib><creatorcontrib>Hauss, Johann</creatorcontrib><creatorcontrib>Witzigmann, Helmut</creatorcontrib><title>Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM： To evaluate the diagnostic potential of cytokeratin-19 （CK-19） mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.</description><subject>Biomarkers, Tumor - analysis</subject><subject>Biomarkers, Tumor - blood</subject><subject>Bone Marrow - pathology</subject><subject>Carcinoma, Pancreatic Ductal - diagnosis</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>Clinical Research</subject><subject>Humans</subject><subject>Keratin-19 - genetics</subject><subject>Neoplastic Cells, Circulating - chemistry</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Pancreatic Neoplasms - diagnosis</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Peritoneal Lavage</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Neoplasm - analysis</subject><subject>RNA, Neoplasm - blood</subject><subject>细胞角蛋白</subject><subject>胰腺炎</subject><subject>腹腔灌洗</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkl-PEyEUxSdG43ZXX300xBifnHphOjPwsompf5NNNJv1mdwBpqU7Ay3QNv1c-wWladX1iXA5_M6Be4viFYVp1c74h_1qMd3RamrZlNXtk2LCGBUl4zN4WkwoQFuKirUXxWWMKwBWVTV7XlzQlrFGQD0pHj6ZZFSy3hHfE21jNKN1mIwma3QqGExWEWWGIZLuQHBcD7a3Cv_cUIfk703Ie1dSQfY2Lclmiy7ZlGs7Q27vyp_zW2Id6Qbv9XvSeWfIiCH4PUGXbUywKddwIAPucGGO2MfeGJR1fsRcTNa4FF8Uz3oconl5Xq-KX18-382_lTc_vn6ff7wpVVULWjaacqMFih45tFrovuGUdw3lQnegG814Y3TPegChqWqo0j1wDtBpnKFpqqvi-sRdb7vRaJW9Aw5yHWyOf5Aerfz_xNmlXPidnEFTi3qWAW9PgD26Ht1Crvw2uBxZ5rax3BxgQGmWvTv7BL_ZmpjkaOPxy9EZv42y4YIBhyNvehKq4GMMpv-bhYI8jsORK_M4SMtkHod84fXjF_yTn_ufBW_OxKV3i43NGTtU970djGRVC1CLtvoNJorCdg</recordid><startdate>20070114</startdate><enddate>20070114</enddate><creator>Hoffmann, Katrin</creator><creator>Kerner, Christiane</creator><creator>Wilfert, Wolfgang</creator><creator>Mueller, Marc</creator><creator>Thiery, Joachim</creator><creator>Hauss, Johann</creator><creator>Witzigmann, Helmut</creator><general>Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Germany%Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Germany</general><general>Baishideng Publishing Group Co., Limited</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20070114</creationdate><title>Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients</title><author>Hoffmann, Katrin ; 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METHODS： Sixty-eight patients with pancreatic cancer （/7 = 37）, chronic pancreatitis （n = 16）, and non-pancreatic benign surgical diseases （/7 = 15, control group） were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS： CK-19 mRNA expression was increased in 24 （64%） blood samples and 11 （30%） of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 （40%） of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors （G2/G3）. Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancer patients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION： Disseminated tumor cells can be detected in patients with pancreatic ductal adenocarcinorna by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers.</abstract><cop>United States</cop><pub>Department of Visceral, Transplantation, Thoracic and Vascular Surgery, University of Leipzig, Germany%Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Germany</pub><pmid>17226905</pmid><doi>10.3748/wjg.v13.i2.257</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarkers, Tumor - analysis Biomarkers, Tumor - blood Bone Marrow - pathology Carcinoma, Pancreatic Ductal - diagnosis Carcinoma, Pancreatic Ductal - pathology Clinical Research Humans Keratin-19 - genetics Neoplastic Cells, Circulating - chemistry Neoplastic Cells, Circulating - pathology Pancreatic Neoplasms - diagnosis Pancreatic Neoplasms - pathology Peritoneal Lavage Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - analysis RNA, Messenger - blood RNA, Neoplasm - analysis RNA, Neoplasm - blood 细胞角蛋白 胰腺炎 腹腔灌洗 |
| title | Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients |
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