Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan
We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes reve...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2014, Vol.76(4), pp.601-604 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | ITO, Hiroya KATSURAGI, Kiyohito AKAMA, Shunsuke YUZAWA, Hirofumi |
| description | We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR. |
| doi_str_mv | 10.1292/jvms.13-0245 |
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Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.13-0245</identifier><identifier>PMID: 24369182</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Actinobacillus Infections - veterinary ; Actinobacillus pleuropneumoniae ; Actinobacillus pleuropneumoniae - genetics ; Animals ; atypical serotype 6 ; Bacteriology ; Base Sequence ; capsular polysaccharide export and biosynthesis genes ; DNA Primers - genetics ; Genotype ; Japan ; Molecular Sequence Data ; multiplex PCR ; Multiplex Polymerase Chain Reaction - veterinary ; nucleotide sequence ; Polysaccharides, Bacterial - genetics ; Recombinant Proteins - genetics ; Sequence Analysis, DNA - veterinary ; Serogroup ; Swine ; Swine Diseases - microbiology</subject><ispartof>Journal of Veterinary Medical Science, 2014, Vol.76(4), pp.601-604</ispartof><rights>2014 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2014</rights><rights>2014 The Japanese Society of Veterinary Science 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c689t-3bb45721228f15f71217ac7c7bae9268f2d04386199ace7137456dccc3db32f63</citedby><cites>FETCH-LOGICAL-c689t-3bb45721228f15f71217ac7c7bae9268f2d04386199ace7137456dccc3db32f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064151/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064151/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24369182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ITO, Hiroya</creatorcontrib><creatorcontrib>KATSURAGI, Kiyohito</creatorcontrib><creatorcontrib>AKAMA, Shunsuke</creatorcontrib><creatorcontrib>YUZAWA, Hirofumi</creatorcontrib><title>Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR.</description><subject>Actinobacillus Infections - veterinary</subject><subject>Actinobacillus pleuropneumoniae</subject><subject>Actinobacillus pleuropneumoniae - genetics</subject><subject>Animals</subject><subject>atypical serotype 6</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>capsular polysaccharide export and biosynthesis genes</subject><subject>DNA Primers - genetics</subject><subject>Genotype</subject><subject>Japan</subject><subject>Molecular Sequence Data</subject><subject>multiplex PCR</subject><subject>Multiplex Polymerase Chain Reaction - veterinary</subject><subject>nucleotide sequence</subject><subject>Polysaccharides, Bacterial - genetics</subject><subject>Recombinant Proteins - genetics</subject><subject>Sequence Analysis, DNA - veterinary</subject><subject>Serogroup</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUFr3DAQRkVpaTab3noOhl56qFONJEvWJbANTZoSKDTtWchaOdEiS45kB_Lva-PtkuSiEczjMTMfQh8BnwGR5OvusctnQEtMWPUGrYAyUQpG5Vu0whJ4KUiFj9BxzjuMCTAu36MjwiiXUJMV-n2do9eDi6GIbbEZnnpntC-ubIjT3xYbM7gQG22c92Muem_HFPtgxy4Gp21xa9MC8sKF4qfudThB71rts_2wr2v09_L7n4sf5c2vq-uLzU1peC2HkjYNqwQBQuoWqlYAAaGNMKLRVhJet2SLGa05SKmNFUAFq_jWGEO3DSUtp2t0vnj7sens1tgwJO1Vn1yn05OK2qmXneDu1V18VAxzBhVMgs97QYoPo82D6lw21nsdbByzgooAJYxN11qjT6_QXRxTmNabKVxXWEo6UV8WyqSYc7LtYRjAag5LzWEpoGoOa8JPny9wgP-nMwHfFmCXB31nD4BOgzPeLjbBFZufvfXQNPc6KRvoPy6dqOM</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>ITO, Hiroya</creator><creator>KATSURAGI, Kiyohito</creator><creator>AKAMA, Shunsuke</creator><creator>YUZAWA, Hirofumi</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2014</creationdate><title>Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan</title><author>ITO, Hiroya ; KATSURAGI, Kiyohito ; AKAMA, Shunsuke ; YUZAWA, Hirofumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c689t-3bb45721228f15f71217ac7c7bae9268f2d04386199ace7137456dccc3db32f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Actinobacillus Infections - veterinary</topic><topic>Actinobacillus pleuropneumoniae</topic><topic>Actinobacillus pleuropneumoniae - genetics</topic><topic>Animals</topic><topic>atypical serotype 6</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>capsular polysaccharide export and biosynthesis genes</topic><topic>DNA Primers - genetics</topic><topic>Genotype</topic><topic>Japan</topic><topic>Molecular Sequence Data</topic><topic>multiplex PCR</topic><topic>Multiplex Polymerase Chain Reaction - veterinary</topic><topic>nucleotide sequence</topic><topic>Polysaccharides, Bacterial - genetics</topic><topic>Recombinant Proteins - genetics</topic><topic>Sequence Analysis, DNA - veterinary</topic><topic>Serogroup</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ITO, Hiroya</creatorcontrib><creatorcontrib>KATSURAGI, Kiyohito</creatorcontrib><creatorcontrib>AKAMA, Shunsuke</creatorcontrib><creatorcontrib>YUZAWA, Hirofumi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ITO, Hiroya</au><au>KATSURAGI, Kiyohito</au><au>AKAMA, Shunsuke</au><au>YUZAWA, Hirofumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2014</date><risdate>2014</risdate><volume>76</volume><issue>4</issue><spage>601</spage><epage>604</epage><pages>601-604</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>24369182</pmid><doi>10.1292/jvms.13-0245</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Actinobacillus Infections - veterinary Actinobacillus pleuropneumoniae Actinobacillus pleuropneumoniae - genetics Animals atypical serotype 6 Bacteriology Base Sequence capsular polysaccharide export and biosynthesis genes DNA Primers - genetics Genotype Japan Molecular Sequence Data multiplex PCR Multiplex Polymerase Chain Reaction - veterinary nucleotide sequence Polysaccharides, Bacterial - genetics Recombinant Proteins - genetics Sequence Analysis, DNA - veterinary Serogroup Swine Swine Diseases - microbiology |
| title | Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan |
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