Development of Duplex PCR Assay for Detection and Differentiation of Typical and Atypical Melissococcus plutonius strains

Melissococcus plutonius is the causative agent of an important honeybee disease, European foulbrood (EFB). In addition to M. plutonius strains with typical characteristics (typical M. plutonius), we recently reported the presence of atypical M. plutonius, which are phenotypically and genetically dis...

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Veröffentlicht in:	Journal of Veterinary Medical Science 2014, Vol.76(4), pp.491-498
Hauptverfasser:	ARAI, Rie, MIYOSHI-AKIYAMA, Tohru, OKUMURA, Kayo, MORINAGA, Yuiko, WU, Meihua, SUGIMURA, Yuya, YOSHIYAMA, Mikio, OKURA, Masatoshi, KIRIKAE, Teruo, TAKAMATSU, Daisuke
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals atypical strain Bacterial Proteins - genetics Bacteriology Base Sequence Bees - microbiology DNA Primers - genetics duplex PCR Enterococcaceae - genetics European foulbrood Gram-Positive Bacterial Infections - diagnosis Melissococcus plutonius Molecular Sequence Data Multiplex Polymerase Chain Reaction - methods Multiplex Polymerase Chain Reaction - veterinary RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Sequence Analysis, DNA - veterinary Sodium-Hydrogen Exchangers - genetics Species Specificity Trans-Activators - genetics typical strain
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