Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering
Macroscale scaffolds created from cartilage‐derived matrix (CDM) demonstrate chondroinductive or chondro‐inductive properties, but many fabrication methods do not allow for control of nanoscale architecture. In this regard, electrospun scaffolds have shown significant promise for cartilage tissue en...
Gespeichert in:
Veröffentlicht in: | Journal of biomedical materials research. Part A 2014-11, Vol.102 (11), p.3998-4008 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 4008 |
---|---|
container_issue | 11 |
container_start_page | 3998 |
container_title | Journal of biomedical materials research. Part A |
container_volume | 102 |
creator | Garrigues, N. William Little, Dianne Sanchez-Adams, Johannah Ruch, David S. Guilak, Farshid |
description | Macroscale scaffolds created from cartilage‐derived matrix (CDM) demonstrate chondroinductive or chondro‐inductive properties, but many fabrication methods do not allow for control of nanoscale architecture. In this regard, electrospun scaffolds have shown significant promise for cartilage tissue engineering. However, nanofibrous materials generally exhibit a relatively small pore size and require techniques such as multilayering or the inclusion of sacrificial fibers to enhance cellular infiltration. The objectives of this study were (1) to compare multilayer to single‐layer electrospun poly(ɛ‐caprolactone) (PCL) scaffolds for cartilage tissue engineering, and (2) to determine whether incorporation of CDM into the PCL fibers would enhance chondrogenesis by human adipose‐derived stem cells (hASCs). PCL and PCL–CDM scaffolds were prepared by sequential collection of 60 electrospun layers from the surface of a grounded saline bath into a single scaffold, or by continuous electrospinning onto the surface of a grounded saline bath and harvest as a single‐layer scaffold. Scaffolds were seeded with hASCs and evaluated over 28 days in culture. The predominant effects on hASCs of incorporation of CDM into scaffolds were to stimulate sulfated glycosaminoglycan synthesis and COL10A1 gene expression. Compared with single‐layer scaffolds, multilayer scaffolds enhanced cell infiltration and ACAN gene expression. However, compared with single‐layer constructs, multilayer PCL constructs had a much lower elastic modulus, and PCL–CDM constructs had an elastic modulus approximately 1% that of PCL constructs. These data suggest that multilayer electrospun constructs enhance homogeneous cell seeding, and that the inclusion of CDM stimulates chondrogenesis‐related bioactivity. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3998–4008, 2014. |
doi_str_mv | 10.1002/jbm.a.35068 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4063882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1664203046</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6578-d8eea8883042f624327315700346088477d4706463395fa5e4a1242b34a235c53</originalsourceid><addsrcrecordid>eNqNkktv1DAUhSMEoi9W7FEkhNRNBr8fG6S2agultBtQl9adxBk8OM5gJ338e5zOMAVWrHyl-53j4-tbFK8xmmGEyPvlvJvBjHIk1LNiF3NOKqYFfz7VTFeUaLFT7KW0zLBAnLwsdgijkmuNd4vrU2_rIfZpNYayhjg4DwtbNTa6W9uUHQzR3ZephrbtfZPKto9PWDm4lEZb2rBwwWZJWBwUL1rwyb7anPvFt7PTrycfq8vr808nR5dVLbhUVaOsBaUURYy0IschkmIuEaJMIKWYlA2TSDBBqeYtcMsAE0bmlAGhvOZ0v_iw9l2N8842tQ1DBG9W0XUQH0wPzvzdCe67WfS3hiFBlSLZ4HBjEPufo02D6VyqrfcQbD8mg4WUmjIm2X-gghGUnyIy-vYfdNmPMeRJTBTCmmox3f3mz_Db1L-_JQPvNgDk0fs2QqhdeuKUVgLJyYisuTvn7cO2j5GZNsPkzTBgHjfDXBx_OXqssqhai1wa7P1WBPGHETIHMDdX5-YzvyFKXBBzTH8Ba_e4ug</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1660193962</pqid></control><display><type>article</type><title>Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Garrigues, N. William ; Little, Dianne ; Sanchez-Adams, Johannah ; Ruch, David S. ; Guilak, Farshid</creator><creatorcontrib>Garrigues, N. William ; Little, Dianne ; Sanchez-Adams, Johannah ; Ruch, David S. ; Guilak, Farshid</creatorcontrib><description>Macroscale scaffolds created from cartilage‐derived matrix (CDM) demonstrate chondroinductive or chondro‐inductive properties, but many fabrication methods do not allow for control of nanoscale architecture. In this regard, electrospun scaffolds have shown significant promise for cartilage tissue engineering. However, nanofibrous materials generally exhibit a relatively small pore size and require techniques such as multilayering or the inclusion of sacrificial fibers to enhance cellular infiltration. The objectives of this study were (1) to compare multilayer to single‐layer electrospun poly(ɛ‐caprolactone) (PCL) scaffolds for cartilage tissue engineering, and (2) to determine whether incorporation of CDM into the PCL fibers would enhance chondrogenesis by human adipose‐derived stem cells (hASCs). PCL and PCL–CDM scaffolds were prepared by sequential collection of 60 electrospun layers from the surface of a grounded saline bath into a single scaffold, or by continuous electrospinning onto the surface of a grounded saline bath and harvest as a single‐layer scaffold. Scaffolds were seeded with hASCs and evaluated over 28 days in culture. The predominant effects on hASCs of incorporation of CDM into scaffolds were to stimulate sulfated glycosaminoglycan synthesis and COL10A1 gene expression. Compared with single‐layer scaffolds, multilayer scaffolds enhanced cell infiltration and ACAN gene expression. However, compared with single‐layer constructs, multilayer PCL constructs had a much lower elastic modulus, and PCL–CDM constructs had an elastic modulus approximately 1% that of PCL constructs. These data suggest that multilayer electrospun constructs enhance homogeneous cell seeding, and that the inclusion of CDM stimulates chondrogenesis‐related bioactivity. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3998–4008, 2014.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.35068</identifier><identifier>PMID: 24375991</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - metabolism ; Adult ; Aggrecans - biosynthesis ; Animals ; Biological and medical sciences ; Biotechnology ; Cartilage ; Cartilage - chemistry ; Cartilage - cytology ; Cartilage - metabolism ; Cells, Cultured ; chondrogenesis ; Collagen Type XI - biosynthesis ; Construction ; Controllers ; Diseases of the osteoarticular system ; Elastic Modulus ; Electrospinning ; electrospun ; extracellular matrix ; Extracellular Matrix - chemistry ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Glycosaminoglycans - biosynthesis ; Health. Pharmaceutical industry ; Humans ; Industrial applications and implications. Economical aspects ; Medical sciences ; mesenchymal stem cell ; Middle Aged ; Miscellaneous ; Multilayers ; nanofiber ; Nanofibers - chemistry ; Nanostructure ; Osteoarthritis ; Polyesters - chemistry ; Porosity ; Programmable logic devices ; Scaffolds ; Stem Cells - cytology ; Stem Cells - metabolism ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Technology. Biomaterials. Equipments ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry</subject><ispartof>Journal of biomedical materials research. Part A, 2014-11, Vol.102 (11), p.3998-4008</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6578-d8eea8883042f624327315700346088477d4706463395fa5e4a1242b34a235c53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.35068$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.35068$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28986072$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24375991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garrigues, N. William</creatorcontrib><creatorcontrib>Little, Dianne</creatorcontrib><creatorcontrib>Sanchez-Adams, Johannah</creatorcontrib><creatorcontrib>Ruch, David S.</creatorcontrib><creatorcontrib>Guilak, Farshid</creatorcontrib><title>Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering</title><title>Journal of biomedical materials research. Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Macroscale scaffolds created from cartilage‐derived matrix (CDM) demonstrate chondroinductive or chondro‐inductive properties, but many fabrication methods do not allow for control of nanoscale architecture. In this regard, electrospun scaffolds have shown significant promise for cartilage tissue engineering. However, nanofibrous materials generally exhibit a relatively small pore size and require techniques such as multilayering or the inclusion of sacrificial fibers to enhance cellular infiltration. The objectives of this study were (1) to compare multilayer to single‐layer electrospun poly(ɛ‐caprolactone) (PCL) scaffolds for cartilage tissue engineering, and (2) to determine whether incorporation of CDM into the PCL fibers would enhance chondrogenesis by human adipose‐derived stem cells (hASCs). PCL and PCL–CDM scaffolds were prepared by sequential collection of 60 electrospun layers from the surface of a grounded saline bath into a single scaffold, or by continuous electrospinning onto the surface of a grounded saline bath and harvest as a single‐layer scaffold. Scaffolds were seeded with hASCs and evaluated over 28 days in culture. The predominant effects on hASCs of incorporation of CDM into scaffolds were to stimulate sulfated glycosaminoglycan synthesis and COL10A1 gene expression. Compared with single‐layer scaffolds, multilayer scaffolds enhanced cell infiltration and ACAN gene expression. However, compared with single‐layer constructs, multilayer PCL constructs had a much lower elastic modulus, and PCL–CDM constructs had an elastic modulus approximately 1% that of PCL constructs. These data suggest that multilayer electrospun constructs enhance homogeneous cell seeding, and that the inclusion of CDM stimulates chondrogenesis‐related bioactivity. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3998–4008, 2014.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - metabolism</subject><subject>Adult</subject><subject>Aggrecans - biosynthesis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cartilage</subject><subject>Cartilage - chemistry</subject><subject>Cartilage - cytology</subject><subject>Cartilage - metabolism</subject><subject>Cells, Cultured</subject><subject>chondrogenesis</subject><subject>Collagen Type XI - biosynthesis</subject><subject>Construction</subject><subject>Controllers</subject><subject>Diseases of the osteoarticular system</subject><subject>Elastic Modulus</subject><subject>Electrospinning</subject><subject>electrospun</subject><subject>extracellular matrix</subject><subject>Extracellular Matrix - chemistry</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Glycosaminoglycans - biosynthesis</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Medical sciences</subject><subject>mesenchymal stem cell</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Multilayers</subject><subject>nanofiber</subject><subject>Nanofibers - chemistry</subject><subject>Nanostructure</subject><subject>Osteoarthritis</subject><subject>Polyesters - chemistry</subject><subject>Porosity</subject><subject>Programmable logic devices</subject><subject>Scaffolds</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1DAUhSMEoi9W7FEkhNRNBr8fG6S2agultBtQl9adxBk8OM5gJ338e5zOMAVWrHyl-53j4-tbFK8xmmGEyPvlvJvBjHIk1LNiF3NOKqYFfz7VTFeUaLFT7KW0zLBAnLwsdgijkmuNd4vrU2_rIfZpNYayhjg4DwtbNTa6W9uUHQzR3ZephrbtfZPKto9PWDm4lEZb2rBwwWZJWBwUL1rwyb7anPvFt7PTrycfq8vr808nR5dVLbhUVaOsBaUURYy0IschkmIuEaJMIKWYlA2TSDBBqeYtcMsAE0bmlAGhvOZ0v_iw9l2N8842tQ1DBG9W0XUQH0wPzvzdCe67WfS3hiFBlSLZ4HBjEPufo02D6VyqrfcQbD8mg4WUmjIm2X-gghGUnyIy-vYfdNmPMeRJTBTCmmox3f3mz_Db1L-_JQPvNgDk0fs2QqhdeuKUVgLJyYisuTvn7cO2j5GZNsPkzTBgHjfDXBx_OXqssqhai1wa7P1WBPGHETIHMDdX5-YzvyFKXBBzTH8Ba_e4ug</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Garrigues, N. William</creator><creator>Little, Dianne</creator><creator>Sanchez-Adams, Johannah</creator><creator>Ruch, David S.</creator><creator>Guilak, Farshid</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>201411</creationdate><title>Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering</title><author>Garrigues, N. William ; Little, Dianne ; Sanchez-Adams, Johannah ; Ruch, David S. ; Guilak, Farshid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6578-d8eea8883042f624327315700346088477d4706463395fa5e4a1242b34a235c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - metabolism</topic><topic>Adult</topic><topic>Aggrecans - biosynthesis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cartilage</topic><topic>Cartilage - chemistry</topic><topic>Cartilage - cytology</topic><topic>Cartilage - metabolism</topic><topic>Cells, Cultured</topic><topic>chondrogenesis</topic><topic>Collagen Type XI - biosynthesis</topic><topic>Construction</topic><topic>Controllers</topic><topic>Diseases of the osteoarticular system</topic><topic>Elastic Modulus</topic><topic>Electrospinning</topic><topic>electrospun</topic><topic>extracellular matrix</topic><topic>Extracellular Matrix - chemistry</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Glycosaminoglycans - biosynthesis</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Medical sciences</topic><topic>mesenchymal stem cell</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Multilayers</topic><topic>nanofiber</topic><topic>Nanofibers - chemistry</topic><topic>Nanostructure</topic><topic>Osteoarthritis</topic><topic>Polyesters - chemistry</topic><topic>Porosity</topic><topic>Programmable logic devices</topic><topic>Scaffolds</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garrigues, N. William</creatorcontrib><creatorcontrib>Little, Dianne</creatorcontrib><creatorcontrib>Sanchez-Adams, Johannah</creatorcontrib><creatorcontrib>Ruch, David S.</creatorcontrib><creatorcontrib>Guilak, Farshid</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garrigues, N. William</au><au>Little, Dianne</au><au>Sanchez-Adams, Johannah</au><au>Ruch, David S.</au><au>Guilak, Farshid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2014-11</date><risdate>2014</risdate><volume>102</volume><issue>11</issue><spage>3998</spage><epage>4008</epage><pages>3998-4008</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Macroscale scaffolds created from cartilage‐derived matrix (CDM) demonstrate chondroinductive or chondro‐inductive properties, but many fabrication methods do not allow for control of nanoscale architecture. In this regard, electrospun scaffolds have shown significant promise for cartilage tissue engineering. However, nanofibrous materials generally exhibit a relatively small pore size and require techniques such as multilayering or the inclusion of sacrificial fibers to enhance cellular infiltration. The objectives of this study were (1) to compare multilayer to single‐layer electrospun poly(ɛ‐caprolactone) (PCL) scaffolds for cartilage tissue engineering, and (2) to determine whether incorporation of CDM into the PCL fibers would enhance chondrogenesis by human adipose‐derived stem cells (hASCs). PCL and PCL–CDM scaffolds were prepared by sequential collection of 60 electrospun layers from the surface of a grounded saline bath into a single scaffold, or by continuous electrospinning onto the surface of a grounded saline bath and harvest as a single‐layer scaffold. Scaffolds were seeded with hASCs and evaluated over 28 days in culture. The predominant effects on hASCs of incorporation of CDM into scaffolds were to stimulate sulfated glycosaminoglycan synthesis and COL10A1 gene expression. Compared with single‐layer scaffolds, multilayer scaffolds enhanced cell infiltration and ACAN gene expression. However, compared with single‐layer constructs, multilayer PCL constructs had a much lower elastic modulus, and PCL–CDM constructs had an elastic modulus approximately 1% that of PCL constructs. These data suggest that multilayer electrospun constructs enhance homogeneous cell seeding, and that the inclusion of CDM stimulates chondrogenesis‐related bioactivity. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3998–4008, 2014.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>24375991</pmid><doi>10.1002/jbm.a.35068</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1549-3296 |
ispartof | Journal of biomedical materials research. Part A, 2014-11, Vol.102 (11), p.3998-4008 |
issn | 1549-3296 1552-4965 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4063882 |
source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
subjects | Adipose Tissue - cytology Adipose Tissue - metabolism Adult Aggrecans - biosynthesis Animals Biological and medical sciences Biotechnology Cartilage Cartilage - chemistry Cartilage - cytology Cartilage - metabolism Cells, Cultured chondrogenesis Collagen Type XI - biosynthesis Construction Controllers Diseases of the osteoarticular system Elastic Modulus Electrospinning electrospun extracellular matrix Extracellular Matrix - chemistry Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation Glycosaminoglycans - biosynthesis Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Medical sciences mesenchymal stem cell Middle Aged Miscellaneous Multilayers nanofiber Nanofibers - chemistry Nanostructure Osteoarthritis Polyesters - chemistry Porosity Programmable logic devices Scaffolds Stem Cells - cytology Stem Cells - metabolism Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Technology. Biomaterials. Equipments Tissue engineering Tissue Engineering - methods Tissue Scaffolds - chemistry |
title | Electrospun cartilage-derived matrix scaffolds for cartilage tissue engineering |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T11%3A48%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrospun%20cartilage-derived%20matrix%20scaffolds%20for%20cartilage%20tissue%20engineering&rft.jtitle=Journal%20of%20biomedical%20materials%20research.%20Part%20A&rft.au=Garrigues,%20N.%20William&rft.date=2014-11&rft.volume=102&rft.issue=11&rft.spage=3998&rft.epage=4008&rft.pages=3998-4008&rft.issn=1549-3296&rft.eissn=1552-4965&rft_id=info:doi/10.1002/jbm.a.35068&rft_dat=%3Cproquest_pubme%3E1664203046%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1660193962&rft_id=info:pmid/24375991&rfr_iscdi=true |