Endoplasmic Reticulum Stress Triggers Gametocytogenesis in the Malaria Parasite

The malaria parasite experiences a significant amount of redox stress during its growth in human erythrocytes and heavily relies on secretory functions for pathogenesis. Most certainly, the parasite is equipped with machinery to tackle perturbations in the secretory pathway, like the unfolded protein response pathway in higher eukaryotes. Our bioinformatics analysis revealed the complete absence of genes involved in the canonical unfolded protein response pathway in Plasmodium falciparum. Accordingly, the parasite was unable to up-regulate endoplasmic reticulum (ER) chaperones or ER-associated degradation in response to DTT-mediated ER stress. Global profiling of gene expression upon DTT treatment revealed a network of AP2 transcription factors and their targets being activated. The overall outcome was up-regulation of genes involved in protein export and the sexual stage of the parasite life cycle culminating in gametocytogenesis. Our results suggest that the malaria parasite uses ER stress as a cue to switch to the transmissible sexual stages. Background: Malaria pathogenesis depends on intricate functioning of ER secretory pathway; however, it lacks canonical UPR machinery to respond to ER stress. Results: Malaria parasite fails to induce ER chaperones and converts to gametocytes upon ER stress. Conclusion: Malaria parasite escapes ER stress by switching to the sexual stage. Significance: ER stress may serve as a physiological cue for parasites to undergo gametocytogenesis.