Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22
Amylase production and biochemical characterization of the crude enzyme preparation from Pseudomonas stutzeri AS22 were evaluated. The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8...
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| Veröffentlicht in: | BioMed research international 2014-01, Vol.2014 (2014), p.1-11 |
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| creator | Nasri, Moncef Ben Ayed, Hanen Ghorbel-Bellaaj, Olfa Hmidet, Noomen Maalej, Hana |
| description | Amylase production and biochemical characterization of the crude enzyme preparation from Pseudomonas stutzeri AS22 were evaluated. The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crude α-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that both α-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups. |
| doi_str_mv | 10.1155/2014/156438 |
| format | Article |
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The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crude α-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that both α-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/156438</identifier><identifier>PMID: 24963472</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>alpha-Glucosidases - biosynthesis ; alpha-Glucosidases - chemistry ; alpha-Glucosidases - isolation & purification ; Amylases ; Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - chemistry ; Bacterial Proteins - isolation & purification ; Bacteriology ; Biomedical research ; Carbohydrates ; Chemical properties ; Enzymes ; Maltose - analogs & derivatives ; Maltose - biosynthesis ; Physiological aspects ; Pseudomonas ; Pseudomonas stutzeri ; Pseudomonas stutzeri - enzymology ; Pseudomonas stutzeri - growth & development ; Quantitative genetics ; Solanum tuberosum</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-11</ispartof><rights>Copyright © 2014 Hana Maalej et al.</rights><rights>COPYRIGHT 2014 John Wiley & Sons, Inc.</rights><rights>Copyright © 2014 Hana Maalej et al. Hana Maalej et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 Hana Maalej et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-22a4dc6ed9c9ab98e19173dc11d58f717dac2667d414ca09fa6090c49c5f8c253</citedby><cites>FETCH-LOGICAL-c490t-22a4dc6ed9c9ab98e19173dc11d58f717dac2667d414ca09fa6090c49c5f8c253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055539/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055539/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24963472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ghorbel, Sofiane</contributor><creatorcontrib>Nasri, Moncef</creatorcontrib><creatorcontrib>Ben Ayed, Hanen</creatorcontrib><creatorcontrib>Ghorbel-Bellaaj, Olfa</creatorcontrib><creatorcontrib>Hmidet, Noomen</creatorcontrib><creatorcontrib>Maalej, Hana</creatorcontrib><title>Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Amylase production and biochemical characterization of the crude enzyme preparation from Pseudomonas stutzeri AS22 were evaluated. The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crude α-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that both α-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.</description><subject>alpha-Glucosidases - biosynthesis</subject><subject>alpha-Glucosidases - chemistry</subject><subject>alpha-Glucosidases - isolation & purification</subject><subject>Amylases</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacteriology</subject><subject>Biomedical research</subject><subject>Carbohydrates</subject><subject>Chemical properties</subject><subject>Enzymes</subject><subject>Maltose - analogs & derivatives</subject><subject>Maltose - biosynthesis</subject><subject>Physiological aspects</subject><subject>Pseudomonas</subject><subject>Pseudomonas stutzeri</subject><subject>Pseudomonas stutzeri - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nasri, Moncef</au><au>Ben Ayed, Hanen</au><au>Ghorbel-Bellaaj, Olfa</au><au>Hmidet, Noomen</au><au>Maalej, Hana</au><au>Ghorbel, Sofiane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Amylase production and biochemical characterization of the crude enzyme preparation from Pseudomonas stutzeri AS22 were evaluated. The highest α-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crude α-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that both α-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>24963472</pmid><doi>10.1155/2014/156438</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alpha-Glucosidases - biosynthesis alpha-Glucosidases - chemistry alpha-Glucosidases - isolation & purification Amylases Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacteriology Biomedical research Carbohydrates Chemical properties Enzymes Maltose - analogs & derivatives Maltose - biosynthesis Physiological aspects Pseudomonas Pseudomonas stutzeri Pseudomonas stutzeri - enzymology Pseudomonas stutzeri - growth & development Quantitative genetics Solanum tuberosum |
| title | Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22 |
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