Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode
A stripping method for the determination of xanthine in the presence of hypoxanthine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the sur...
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| Veröffentlicht in: | Analytical Chemistry Insights 2014, Vol.2014 (9), p.49-55 |
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| creator | Farias, Percio Augusto Mardini Castro, Arnaldo Aguiar |
| description | A stripping method for the determination of xanthine in the presence of hypoxanthine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10−3 mol L−1 NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and −0.50 V for hypoxanthine–copper, and a linear scan rate of 200 mV second−1. The response of xanthine is linear over the concentration ranges of 20-140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10−10 mol L−1). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of xanthine associated with hypoxanthine, uric acid, nitrogenated bases, ATP, and ssDNA. |
| doi_str_mv | 10.4137/ACI.S14712 |
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The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10−3 mol L−1 NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and −0.50 V for hypoxanthine–copper, and a linear scan rate of 200 mV second−1. The response of xanthine is linear over the concentration ranges of 20-140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10−10 mol L−1). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of xanthine associated with hypoxanthine, uric acid, nitrogenated bases, ATP, and ssDNA.</description><identifier>ISSN: 1177-3901</identifier><identifier>EISSN: 1177-3901</identifier><identifier>DOI: 10.4137/ACI.S14712</identifier><identifier>PMID: 24940040</identifier><language>eng</language><publisher>London, England: Libertas Academica</publisher><subject>Adsorptivity ; Dielectric films ; Electrodes ; Electrolytes ; Hypoxanthine ; Optimization ; Original Research ; Stripping ; Thin films ; Uric acid ; Xanthine ; Xanthines</subject><ispartof>Analytical Chemistry Insights, 2014, Vol.2014 (9), p.49-55</ispartof><rights>2014 SAGE Publications.</rights><rights>COPYRIGHT 2014 Sage Publications Ltd. (UK)</rights><rights>Copyright Libertas Academica Ltd 2014</rights><rights>2014 the author(s), publisher and licensee Libertas Academica Ltd. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a5752-8a9bacdbe511d4cacf94fe9e9c76122dcbe4a9095471cdffb3871ba21dcf50fb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055419/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055419/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27902,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24940040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farias, Percio Augusto Mardini</creatorcontrib><creatorcontrib>Castro, Arnaldo Aguiar</creatorcontrib><title>Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode</title><title>Analytical Chemistry Insights</title><addtitle>Anal Chem Insights</addtitle><description>A stripping method for the determination of xanthine in the presence of hypoxanthine at the submicromolar concentration levels is described. The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10−3 mol L−1 NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and −0.50 V for hypoxanthine–copper, and a linear scan rate of 200 mV second−1. The response of xanthine is linear over the concentration ranges of 20-140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10−10 mol L−1). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. The utility of the method is demonstrated by the presence of xanthine associated with hypoxanthine, uric acid, nitrogenated bases, ATP, and ssDNA.</description><subject>Adsorptivity</subject><subject>Dielectric films</subject><subject>Electrodes</subject><subject>Electrolytes</subject><subject>Hypoxanthine</subject><subject>Optimization</subject><subject>Original Research</subject><subject>Stripping</subject><subject>Thin films</subject><subject>Uric acid</subject><subject>Xanthine</subject><subject>Xanthines</subject><issn>1177-3901</issn><issn>1177-3901</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>BENPR</sourceid><recordid>eNqNklFv0zAQxyMEYmPwwgdAkXgAIbXYjh3HL0hV2djQEJMGiDfLcS6tp8QOtjtR8eVxl2600x6QH8539_Pf5_Nl2UuMphQX_P1sfja9xJRj8ig7xJjzSSEQfryzP8iehXCFUIkrVjzNDggVFCGKDrM_HyGC741V0Tibuzb_qWxcGgu5sXlcQn7hIYDVsMmdrgf3-zZfr_NZE5wformG_DJ6MwzGLvIfrouq7yH6da7ijcYX8HqV3BPT9flxBzp618Dz7EmrugAvtvYo-35y_G1-Ojn_-ulsPjufKMYZmVRK1Eo3NTCMG6qVbgVtQYDQvMSENLoGqgQSLHVAN21bFxXHtSK40S1DyT3KPoy6w6ruodFgo1edHLzplV9Lp4zcz1izlAt3LSlijGKRBN5uBbz7tYIQZW-Chq5TFtwqSMyYKCtBCvYfaMGZKGhJEvr6HnrlVt6mTiSKEkRoVe1QC9WBNLZ1qUS9EZWzghec8kpsKpw-QKXVQG-0s9CaFN878G48oL0LwUN71w6M5GaqZJoqOU5Vgl_tNvAOvR2jBLwZgaAWsPOMh6S2f9GZGnxUYVuj2vuOe0mtpHa9pOm-JPB5FFDGm2j-XXZBEC4RSwsRmfYU35htiPJ9hwrJWPEXMvkAmQ</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Farias, Percio Augusto Mardini</creator><creator>Castro, Arnaldo Aguiar</creator><general>Libertas Academica</general><general>SAGE Publishing</general><general>SAGE Publications</general><general>Sage Publications Ltd. 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The method is based on controlled adsorptive accumulation at the thin-film mercury electrode followed by a fast linear scan voltammetric measurement of the surface species. Optimum experimental conditions were found to be the use of 1.0 × 10−3 mol L−1 NaOH solution as supporting electrolyte, an accumulation potential of 0.00 V for xanthine and −0.50 V for hypoxanthine–copper, and a linear scan rate of 200 mV second−1. The response of xanthine is linear over the concentration ranges of 20-140 ppb. For an accumulation time of 30 minutes, the detection limit was found to be 36 ppt (2.3 × 10−10 mol L−1). Adequate conditions for measuring the xanthine in the presence of hypoxanthine, copper and other metals, uric acid, and other nitrogenated bases were also investigated. 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| subjects | Adsorptivity Dielectric films Electrodes Electrolytes Hypoxanthine Optimization Original Research Stripping Thin films Uric acid Xanthine Xanthines |
| title | Determination of Xanthine in the Presence of Hypoxanthine by Adsorptive Stripping Voltammetry at the Mercury Film Electrode |
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