Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase
APOBEC3 proteins are restriction factors that induce G→A hypermutation in retroviruses during replication as a result of cytidine deamination of minus-strand DNA transcripts. However, the mechanism of APOBEC inhibition of murine leukemia viruses (MuLVs) does not appear to be G→A hypermutation and is...
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| Veröffentlicht in: | Journal of virology 2014-07, Vol.88 (13), p.7659-7662 |
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| container_end_page | 7662 |
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| container_issue | 13 |
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| container_title | Journal of virology |
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| creator | Boi, Stefano Kolokithas, Angelo Shepard, Joyce Linwood, Rebecca Rosenke, Kyle Van Dis, Erik Malik, Frank Evans, Leonard H |
| description | APOBEC3 proteins are restriction factors that induce G→A hypermutation in retroviruses during replication as a result of cytidine deamination of minus-strand DNA transcripts. However, the mechanism of APOBEC inhibition of murine leukemia viruses (MuLVs) does not appear to be G→A hypermutation and is unclear. In this report, the incorporation of mA3 in virions resulted in a loss in virion reverse transcriptase (RT) activity and RT fidelity that correlated with the loss of virion-specific infectivity. |
| doi_str_mv | 10.1128/JVI.00967-14 |
| format | Article |
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All Rights Reserved. 2014 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-3dc31fa00e71ac3d600195925883ad35589e14189809c86d1c7a40f31852af203</citedby><cites>FETCH-LOGICAL-c417t-3dc31fa00e71ac3d600195925883ad35589e14189809c86d1c7a40f31852af203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054435/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054435/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27904,27905,53771,53773</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24719421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Beemon, K. 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In this report, the incorporation of mA3 in virions resulted in a loss in virion reverse transcriptase (RT) activity and RT fidelity that correlated with the loss of virion-specific infectivity.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cytidine Deaminase - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Moloney murine leukemia virus - enzymology</subject><subject>Moloney murine leukemia virus - pathogenicity</subject><subject>Murine leukemia virus</subject><subject>Retroviridae Infections - enzymology</subject><subject>Retroviridae Infections - virology</subject><subject>Retrovirus</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><subject>Transfection</subject><subject>Tumor Virus Infections - enzymology</subject><subject>Tumor Virus Infections - virology</subject><subject>Virion - pathogenicity</subject><subject>Virus Assembly</subject><subject>Virus Replication</subject><subject>Virus-Cell Interactions</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT1vFDEQhi0EIkego0YuKdjg8ceu3SCFU4BDkZICEJ1lvLPEsGtf7N2T0vDb4yMhIl0aW9a882jGDyEvgR0BcP3287fNEWOm7RqQj8gKmNGNUiAfkxVjnDdK6O8H5FkpvxgDKVv5lBxw2YGRHFbkzyb6lLcpuzmkSNNAp7QUpMfnZ-9P1oKGOCc6LTlEpCMuv3EKju5CXsr-rC2F9ugzuoKFzhdInZ_DLsxX1MWeDqHHcf-o3Iw7zJU8ZxeLz2E7157n5MngxoIvbu9D8vXDyZf1p-b07ONmfXzaeAnd3IjeCxgcY9iB86Jv6ypGGa60Fq4XSmmDIEEbzYzXbQ--c5INArTibuBMHJJ3N9zt8mPC3mOsY4x2m8Pk8pVNLtj7lRgu7M-0s5IpKYWqgNe3gJwuFyyznULxOI4uYv0wC0qaVkDb8YdEq5fOtFCjb26iPqdSMg53EwGze7u22rV_7VqQNf7q_y3uwv90imuWq6Gt</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Boi, Stefano</creator><creator>Kolokithas, Angelo</creator><creator>Shepard, Joyce</creator><creator>Linwood, Rebecca</creator><creator>Rosenke, Kyle</creator><creator>Van Dis, Erik</creator><creator>Malik, Frank</creator><creator>Evans, Leonard H</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140701</creationdate><title>Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase</title><author>Boi, Stefano ; 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L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase</atitle><jtitle>Journal of virology</jtitle><addtitle>J Virol</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>88</volume><issue>13</issue><spage>7659</spage><epage>7662</epage><pages>7659-7662</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>APOBEC3 proteins are restriction factors that induce G→A hypermutation in retroviruses during replication as a result of cytidine deamination of minus-strand DNA transcripts. However, the mechanism of APOBEC inhibition of murine leukemia viruses (MuLVs) does not appear to be G→A hypermutation and is unclear. 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| subjects | Animals Blotting, Western Cytidine Deaminase - physiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Moloney murine leukemia virus - enzymology Moloney murine leukemia virus - pathogenicity Murine leukemia virus Retroviridae Infections - enzymology Retroviridae Infections - virology Retrovirus RNA-Directed DNA Polymerase - metabolism Transfection Tumor Virus Infections - enzymology Tumor Virus Infections - virology Virion - pathogenicity Virus Assembly Virus Replication Virus-Cell Interactions |
| title | Incorporation of mouse APOBEC3 into murine leukemia virus virions decreases the activity and fidelity of reverse transcriptase |
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