Adaptation of HIV-1 to Its Human Host
Human immunodeficiency virus type 1 (HIV-1) originated from three independent cross-species transmissions of simian immunodeficiency virus (SIVcpzPtt) infecting chimpanzees (Pan troglodytes troglodytes) in west central Africa, giving rise to pandemic (group M) and non-pandemic (groups N and O) clade...
Gespeichert in:
| Veröffentlicht in: | Molecular biology and evolution 2007-08, Vol.24 (8), p.1853-1860 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1860 |
|---|---|
| container_issue | 8 |
| container_start_page | 1853 |
| container_title | Molecular biology and evolution |
| container_volume | 24 |
| creator | Wain, Louise V. Bailes, Elizabeth Bibollet-Ruche, Frederic Decker, Julie M. Keele, Brandon F. Van Heuverswyn, Fran Li, Yingying Takehisa, Jun Ngole, Eitel Mpoudi Shaw, George M. Peeters, Martine Hahn, Beatrice H. Sharp, Paul M. |
| description | Human immunodeficiency virus type 1 (HIV-1) originated from three independent cross-species transmissions of simian immunodeficiency virus (SIVcpzPtt) infecting chimpanzees (Pan troglodytes troglodytes) in west central Africa, giving rise to pandemic (group M) and non-pandemic (groups N and O) clades of HIV-1. To identify host-specific adaptations in HIV-1 we compared the inferred ancestral sequences of HIV-1 groups M, N and O to 12 full length genome sequences of SIVcpzPtt and four of the outlying but closely related SIVcpzPts (from P. t. schweinfurthii). This analysis revealed a single site that was completely conserved among SIVcpzPtt strains but different (due to the same change) in all three groups of HIV-1. This site, Gag-30, lies within p17, the gag-encoded matrix protein. It is Met in SIVcpzPtt, underwent a conservative replacement by Leu in one lineage of SIVcpzPts but changed radically to Arg on all three lineages leading to HIV-1. During subsequent diversification this site has been conserved as a basic residue (Arg or Lys) in most lineages of HIV-1. Retrospective analysis revealed that Gag-30 had reverted to Met in a previous experiment in which HIV-1 was passaged through chimpanzees. To examine whether this substitution conferred a species specific growth advantage, we used site-directed mutagenesis to generate variants of these chimpanzee-adapted HIV-1 strains with Lys at Gag-30, and tested their replication in both human and chimpanzee CD4+ T lymphocytes. Remarkably, viruses encoding Met replicated to higher titers than viruses encoding Lys in chimpanzee T cells, but the opposite was found in human T cells. Taken together, these observations provide compelling evidence for host-specific adaptation during the emergence of HIV-1 and identify the viral matrix protein as a modulator of viral fitness following transmission to the new human host. |
| doi_str_mv | 10.1093/molbev/msm110 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_TOX</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4053193</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/molbev/msm110</oup_id><sourcerecordid>19882768</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-e94a70e0edc3599fdd509ceb8a72fa4175ee46f17963f0ab57a95f26b3b185933</originalsourceid><addsrcrecordid>eNqFkE1LxDAQhoMo7rp69CpFULzUTZqkSS7CsqhdWPCiXkPaJlppm9qkC_57o138uniagXl4ZuYF4BjBSwQFnje2zvVm3rgGIbgDpohiFiOGxC6YQhZ6AjGfgAPnXiBEhKTpPpggRglFnE_B2aJUnVe-sm1kTZStHmMUeRutvIuyoVFtlFnnD8GeUbXTR9s6Aw831_fLLF7f3a6Wi3VcEJb6WAuiGNRQlwWmQpiypFAUOueKJUaRsFVrkhrERIoNVDllSlCTpDnOEacC4xm4Gr3dkDfBolvfq1p2fdWo_k1aVcnfk7Z6lk92IwmkGH0KzreC3r4O2nnZVK7Qda1abQcnkeA8YSkP4Okf8MUOfRuekwlOOMEJFgGKR6jorXO9Nl-XICg_0pdj-nJMP_AnP8__prdxB-BiBOzQ_eN6B28XjrI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>232843239</pqid></control><display><type>article</type><title>Adaptation of HIV-1 to Its Human Host</title><source>Oxford Journals Open Access Collection</source><creator>Wain, Louise V. ; Bailes, Elizabeth ; Bibollet-Ruche, Frederic ; Decker, Julie M. ; Keele, Brandon F. ; Van Heuverswyn, Fran ; Li, Yingying ; Takehisa, Jun ; Ngole, Eitel Mpoudi ; Shaw, George M. ; Peeters, Martine ; Hahn, Beatrice H. ; Sharp, Paul M.</creator><creatorcontrib>Wain, Louise V. ; Bailes, Elizabeth ; Bibollet-Ruche, Frederic ; Decker, Julie M. ; Keele, Brandon F. ; Van Heuverswyn, Fran ; Li, Yingying ; Takehisa, Jun ; Ngole, Eitel Mpoudi ; Shaw, George M. ; Peeters, Martine ; Hahn, Beatrice H. ; Sharp, Paul M.</creatorcontrib><description>Human immunodeficiency virus type 1 (HIV-1) originated from three independent cross-species transmissions of simian immunodeficiency virus (SIVcpzPtt) infecting chimpanzees (Pan troglodytes troglodytes) in west central Africa, giving rise to pandemic (group M) and non-pandemic (groups N and O) clades of HIV-1. To identify host-specific adaptations in HIV-1 we compared the inferred ancestral sequences of HIV-1 groups M, N and O to 12 full length genome sequences of SIVcpzPtt and four of the outlying but closely related SIVcpzPts (from P. t. schweinfurthii). This analysis revealed a single site that was completely conserved among SIVcpzPtt strains but different (due to the same change) in all three groups of HIV-1. This site, Gag-30, lies within p17, the gag-encoded matrix protein. It is Met in SIVcpzPtt, underwent a conservative replacement by Leu in one lineage of SIVcpzPts but changed radically to Arg on all three lineages leading to HIV-1. During subsequent diversification this site has been conserved as a basic residue (Arg or Lys) in most lineages of HIV-1. Retrospective analysis revealed that Gag-30 had reverted to Met in a previous experiment in which HIV-1 was passaged through chimpanzees. To examine whether this substitution conferred a species specific growth advantage, we used site-directed mutagenesis to generate variants of these chimpanzee-adapted HIV-1 strains with Lys at Gag-30, and tested their replication in both human and chimpanzee CD4+ T lymphocytes. Remarkably, viruses encoding Met replicated to higher titers than viruses encoding Lys in chimpanzee T cells, but the opposite was found in human T cells. Taken together, these observations provide compelling evidence for host-specific adaptation during the emergence of HIV-1 and identify the viral matrix protein as a modulator of viral fitness following transmission to the new human host.</description><identifier>ISSN: 0737-4038</identifier><identifier>EISSN: 1537-1719</identifier><identifier>DOI: 10.1093/molbev/msm110</identifier><identifier>PMID: 17545188</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Acquired Immunodeficiency Syndrome - epidemiology ; Acquired Immunodeficiency Syndrome - transmission ; Acquired Immunodeficiency Syndrome - virology ; Adaptation ; Africa, Central ; Animals ; Biological Evolution ; CD4-Positive T-Lymphocytes - metabolism ; CD4-Positive T-Lymphocytes - virology ; Evolutionary biology ; Gene Products, gag - genetics ; Gene Products, gag - metabolism ; Genomics ; HIV ; HIV-1 - physiology ; Human immunodeficiency virus ; Human immunodeficiency virus 1 ; Humans ; Lymphocytes ; Molecular biology ; Mutagenesis, Site-Directed ; Mutation - genetics ; Pan troglodytes ; Pan troglodytes troglodytes ; Pandemics ; Phylogeny ; Primates ; Sequence Analysis, DNA ; Simian Acquired Immunodeficiency Syndrome - epidemiology ; Simian Acquired Immunodeficiency Syndrome - transmission ; Simian Acquired Immunodeficiency Syndrome - virology ; Simian immunodeficiency virus ; Simian Immunodeficiency Virus - classification ; Simian Immunodeficiency Virus - genetics ; Simian Immunodeficiency Virus - isolation & purification ; Virus Replication</subject><ispartof>Molecular biology and evolution, 2007-08, Vol.24 (8), p.1853-1860</ispartof><rights>The Author 2007. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org 2007</rights><rights>The Author 2007. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org</rights><rights>The Author 2007. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-e94a70e0edc3599fdd509ceb8a72fa4175ee46f17963f0ab57a95f26b3b185933</citedby><cites>FETCH-LOGICAL-c476t-e94a70e0edc3599fdd509ceb8a72fa4175ee46f17963f0ab57a95f26b3b185933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,1603,27922,27923</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/molbev/msm110$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17545188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wain, Louise V.</creatorcontrib><creatorcontrib>Bailes, Elizabeth</creatorcontrib><creatorcontrib>Bibollet-Ruche, Frederic</creatorcontrib><creatorcontrib>Decker, Julie M.</creatorcontrib><creatorcontrib>Keele, Brandon F.</creatorcontrib><creatorcontrib>Van Heuverswyn, Fran</creatorcontrib><creatorcontrib>Li, Yingying</creatorcontrib><creatorcontrib>Takehisa, Jun</creatorcontrib><creatorcontrib>Ngole, Eitel Mpoudi</creatorcontrib><creatorcontrib>Shaw, George M.</creatorcontrib><creatorcontrib>Peeters, Martine</creatorcontrib><creatorcontrib>Hahn, Beatrice H.</creatorcontrib><creatorcontrib>Sharp, Paul M.</creatorcontrib><title>Adaptation of HIV-1 to Its Human Host</title><title>Molecular biology and evolution</title><addtitle>Mol Biol Evol</addtitle><description>Human immunodeficiency virus type 1 (HIV-1) originated from three independent cross-species transmissions of simian immunodeficiency virus (SIVcpzPtt) infecting chimpanzees (Pan troglodytes troglodytes) in west central Africa, giving rise to pandemic (group M) and non-pandemic (groups N and O) clades of HIV-1. To identify host-specific adaptations in HIV-1 we compared the inferred ancestral sequences of HIV-1 groups M, N and O to 12 full length genome sequences of SIVcpzPtt and four of the outlying but closely related SIVcpzPts (from P. t. schweinfurthii). This analysis revealed a single site that was completely conserved among SIVcpzPtt strains but different (due to the same change) in all three groups of HIV-1. This site, Gag-30, lies within p17, the gag-encoded matrix protein. It is Met in SIVcpzPtt, underwent a conservative replacement by Leu in one lineage of SIVcpzPts but changed radically to Arg on all three lineages leading to HIV-1. During subsequent diversification this site has been conserved as a basic residue (Arg or Lys) in most lineages of HIV-1. Retrospective analysis revealed that Gag-30 had reverted to Met in a previous experiment in which HIV-1 was passaged through chimpanzees. To examine whether this substitution conferred a species specific growth advantage, we used site-directed mutagenesis to generate variants of these chimpanzee-adapted HIV-1 strains with Lys at Gag-30, and tested their replication in both human and chimpanzee CD4+ T lymphocytes. Remarkably, viruses encoding Met replicated to higher titers than viruses encoding Lys in chimpanzee T cells, but the opposite was found in human T cells. Taken together, these observations provide compelling evidence for host-specific adaptation during the emergence of HIV-1 and identify the viral matrix protein as a modulator of viral fitness following transmission to the new human host.</description><subject>Acquired Immunodeficiency Syndrome - epidemiology</subject><subject>Acquired Immunodeficiency Syndrome - transmission</subject><subject>Acquired Immunodeficiency Syndrome - virology</subject><subject>Adaptation</subject><subject>Africa, Central</subject><subject>Animals</subject><subject>Biological Evolution</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Evolutionary biology</subject><subject>Gene Products, gag - genetics</subject><subject>Gene Products, gag - metabolism</subject><subject>Genomics</subject><subject>HIV</subject><subject>HIV-1 - physiology</subject><subject>Human immunodeficiency virus</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Lymphocytes</subject><subject>Molecular biology</subject><subject>Mutagenesis, Site-Directed</subject><subject>Mutation - genetics</subject><subject>Pan troglodytes</subject><subject>Pan troglodytes troglodytes</subject><subject>Pandemics</subject><subject>Phylogeny</subject><subject>Primates</subject><subject>Sequence Analysis, DNA</subject><subject>Simian Acquired Immunodeficiency Syndrome - epidemiology</subject><subject>Simian Acquired Immunodeficiency Syndrome - transmission</subject><subject>Simian Acquired Immunodeficiency Syndrome - virology</subject><subject>Simian immunodeficiency virus</subject><subject>Simian Immunodeficiency Virus - classification</subject><subject>Simian Immunodeficiency Virus - genetics</subject><subject>Simian Immunodeficiency Virus - isolation & purification</subject><subject>Virus Replication</subject><issn>0737-4038</issn><issn>1537-1719</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkE1LxDAQhoMo7rp69CpFULzUTZqkSS7CsqhdWPCiXkPaJlppm9qkC_57o138uniagXl4ZuYF4BjBSwQFnje2zvVm3rgGIbgDpohiFiOGxC6YQhZ6AjGfgAPnXiBEhKTpPpggRglFnE_B2aJUnVe-sm1kTZStHmMUeRutvIuyoVFtlFnnD8GeUbXTR9s6Aw831_fLLF7f3a6Wi3VcEJb6WAuiGNRQlwWmQpiypFAUOueKJUaRsFVrkhrERIoNVDllSlCTpDnOEacC4xm4Gr3dkDfBolvfq1p2fdWo_k1aVcnfk7Z6lk92IwmkGH0KzreC3r4O2nnZVK7Qda1abQcnkeA8YSkP4Okf8MUOfRuekwlOOMEJFgGKR6jorXO9Nl-XICg_0pdj-nJMP_AnP8__prdxB-BiBOzQ_eN6B28XjrI</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Wain, Louise V.</creator><creator>Bailes, Elizabeth</creator><creator>Bibollet-Ruche, Frederic</creator><creator>Decker, Julie M.</creator><creator>Keele, Brandon F.</creator><creator>Van Heuverswyn, Fran</creator><creator>Li, Yingying</creator><creator>Takehisa, Jun</creator><creator>Ngole, Eitel Mpoudi</creator><creator>Shaw, George M.</creator><creator>Peeters, Martine</creator><creator>Hahn, Beatrice H.</creator><creator>Sharp, Paul M.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20070801</creationdate><title>Adaptation of HIV-1 to Its Human Host</title><author>Wain, Louise V. ; Bailes, Elizabeth ; Bibollet-Ruche, Frederic ; Decker, Julie M. ; Keele, Brandon F. ; Van Heuverswyn, Fran ; Li, Yingying ; Takehisa, Jun ; Ngole, Eitel Mpoudi ; Shaw, George M. ; Peeters, Martine ; Hahn, Beatrice H. ; Sharp, Paul M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-e94a70e0edc3599fdd509ceb8a72fa4175ee46f17963f0ab57a95f26b3b185933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acquired Immunodeficiency Syndrome - epidemiology</topic><topic>Acquired Immunodeficiency Syndrome - transmission</topic><topic>Acquired Immunodeficiency Syndrome - virology</topic><topic>Adaptation</topic><topic>Africa, Central</topic><topic>Animals</topic><topic>Biological Evolution</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Evolutionary biology</topic><topic>Gene Products, gag - genetics</topic><topic>Gene Products, gag - metabolism</topic><topic>Genomics</topic><topic>HIV</topic><topic>HIV-1 - physiology</topic><topic>Human immunodeficiency virus</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Lymphocytes</topic><topic>Molecular biology</topic><topic>Mutagenesis, Site-Directed</topic><topic>Mutation - genetics</topic><topic>Pan troglodytes</topic><topic>Pan troglodytes troglodytes</topic><topic>Pandemics</topic><topic>Phylogeny</topic><topic>Primates</topic><topic>Sequence Analysis, DNA</topic><topic>Simian Acquired Immunodeficiency Syndrome - epidemiology</topic><topic>Simian Acquired Immunodeficiency Syndrome - transmission</topic><topic>Simian Acquired Immunodeficiency Syndrome - virology</topic><topic>Simian immunodeficiency virus</topic><topic>Simian Immunodeficiency Virus - classification</topic><topic>Simian Immunodeficiency Virus - genetics</topic><topic>Simian Immunodeficiency Virus - isolation & purification</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wain, Louise V.</creatorcontrib><creatorcontrib>Bailes, Elizabeth</creatorcontrib><creatorcontrib>Bibollet-Ruche, Frederic</creatorcontrib><creatorcontrib>Decker, Julie M.</creatorcontrib><creatorcontrib>Keele, Brandon F.</creatorcontrib><creatorcontrib>Van Heuverswyn, Fran</creatorcontrib><creatorcontrib>Li, Yingying</creatorcontrib><creatorcontrib>Takehisa, Jun</creatorcontrib><creatorcontrib>Ngole, Eitel Mpoudi</creatorcontrib><creatorcontrib>Shaw, George M.</creatorcontrib><creatorcontrib>Peeters, Martine</creatorcontrib><creatorcontrib>Hahn, Beatrice H.</creatorcontrib><creatorcontrib>Sharp, Paul M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Wain, Louise V.</au><au>Bailes, Elizabeth</au><au>Bibollet-Ruche, Frederic</au><au>Decker, Julie M.</au><au>Keele, Brandon F.</au><au>Van Heuverswyn, Fran</au><au>Li, Yingying</au><au>Takehisa, Jun</au><au>Ngole, Eitel Mpoudi</au><au>Shaw, George M.</au><au>Peeters, Martine</au><au>Hahn, Beatrice H.</au><au>Sharp, Paul M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adaptation of HIV-1 to Its Human Host</atitle><jtitle>Molecular biology and evolution</jtitle><addtitle>Mol Biol Evol</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>24</volume><issue>8</issue><spage>1853</spage><epage>1860</epage><pages>1853-1860</pages><issn>0737-4038</issn><eissn>1537-1719</eissn><abstract>Human immunodeficiency virus type 1 (HIV-1) originated from three independent cross-species transmissions of simian immunodeficiency virus (SIVcpzPtt) infecting chimpanzees (Pan troglodytes troglodytes) in west central Africa, giving rise to pandemic (group M) and non-pandemic (groups N and O) clades of HIV-1. To identify host-specific adaptations in HIV-1 we compared the inferred ancestral sequences of HIV-1 groups M, N and O to 12 full length genome sequences of SIVcpzPtt and four of the outlying but closely related SIVcpzPts (from P. t. schweinfurthii). This analysis revealed a single site that was completely conserved among SIVcpzPtt strains but different (due to the same change) in all three groups of HIV-1. This site, Gag-30, lies within p17, the gag-encoded matrix protein. It is Met in SIVcpzPtt, underwent a conservative replacement by Leu in one lineage of SIVcpzPts but changed radically to Arg on all three lineages leading to HIV-1. During subsequent diversification this site has been conserved as a basic residue (Arg or Lys) in most lineages of HIV-1. Retrospective analysis revealed that Gag-30 had reverted to Met in a previous experiment in which HIV-1 was passaged through chimpanzees. To examine whether this substitution conferred a species specific growth advantage, we used site-directed mutagenesis to generate variants of these chimpanzee-adapted HIV-1 strains with Lys at Gag-30, and tested their replication in both human and chimpanzee CD4+ T lymphocytes. Remarkably, viruses encoding Met replicated to higher titers than viruses encoding Lys in chimpanzee T cells, but the opposite was found in human T cells. Taken together, these observations provide compelling evidence for host-specific adaptation during the emergence of HIV-1 and identify the viral matrix protein as a modulator of viral fitness following transmission to the new human host.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>17545188</pmid><doi>10.1093/molbev/msm110</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 0737-4038 |
| ispartof | Molecular biology and evolution, 2007-08, Vol.24 (8), p.1853-1860 |
| issn | 0737-4038 1537-1719 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4053193 |
| source | Oxford Journals Open Access Collection |
| subjects | Acquired Immunodeficiency Syndrome - epidemiology Acquired Immunodeficiency Syndrome - transmission Acquired Immunodeficiency Syndrome - virology Adaptation Africa, Central Animals Biological Evolution CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - virology Evolutionary biology Gene Products, gag - genetics Gene Products, gag - metabolism Genomics HIV HIV-1 - physiology Human immunodeficiency virus Human immunodeficiency virus 1 Humans Lymphocytes Molecular biology Mutagenesis, Site-Directed Mutation - genetics Pan troglodytes Pan troglodytes troglodytes Pandemics Phylogeny Primates Sequence Analysis, DNA Simian Acquired Immunodeficiency Syndrome - epidemiology Simian Acquired Immunodeficiency Syndrome - transmission Simian Acquired Immunodeficiency Syndrome - virology Simian immunodeficiency virus Simian Immunodeficiency Virus - classification Simian Immunodeficiency Virus - genetics Simian Immunodeficiency Virus - isolation & purification Virus Replication |
| title | Adaptation of HIV-1 to Its Human Host |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T02%3A18%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_TOX&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adaptation%20of%20HIV-1%20to%20Its%20Human%20Host&rft.jtitle=Molecular%20biology%20and%20evolution&rft.au=Wain,%20Louise%20V.&rft.date=2007-08-01&rft.volume=24&rft.issue=8&rft.spage=1853&rft.epage=1860&rft.pages=1853-1860&rft.issn=0737-4038&rft.eissn=1537-1719&rft_id=info:doi/10.1093/molbev/msm110&rft_dat=%3Cproquest_TOX%3E19882768%3C/proquest_TOX%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=232843239&rft_id=info:pmid/17545188&rft_oup_id=10.1093/molbev/msm110&rfr_iscdi=true |