Protein misfolding cyclic amplification of infectious prions

Prions are proteinaceous infectious agents responsible for the transmission of prion diseases. The lack of a procedure for cultivating prions in the laboratory has been a major limitation to the study of the unorthodox nature of this infectious agent and the molecular mechanism by which the normal p...

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Veröffentlicht in:	Nature protocols 2012-06, Vol.7 (7), p.1397-1409
Hauptverfasser:	Morales, Rodrigo, Duran-Aniotz, Claudia, Diaz-Espinoza, Rodrigo, Camacho, Manuel V, Soto, Claudio
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2196 64 64/110 692/699/375/1937 82 Alzheimer's disease Amplification Analytical Chemistry Biological Techniques Blood Blood & organ donations Brain research Computational Biology/Bioinformatics Disease Disease transmission Food industry Food safety Humans In vivo methods and tests Infectivity Life Sciences Microarrays Organic Chemistry Polymers Prion Diseases - genetics Prion protein Prions Prions - chemistry Protein Engineering - methods Protein Folding Protein Multimerization Proteins Protocol Replication Seeds Sonication Sonication - methods Ultrasonic imaging Veterinary diseases
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