Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1

Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Applied and Environmental Microbiology 2004-05, Vol.70 (5), p.2653-2659
Hauptverfasser: Flaherty, J.E, Woloshuk, C.P
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2659
container_issue 5
container_start_page 2653
container_title Applied and Environmental Microbiology
container_volume 70
creator Flaherty, J.E
Woloshuk, C.P
description Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.
doi_str_mv 10.1128/AEM.70.5.2653-2659.2004
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_404460</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16191992</sourcerecordid><originalsourceid>FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</originalsourceid><addsrcrecordid>eNpdkkFv1DAQhSMEokvhL1CDBCcSxk7s2AcOVdUFpCKkQi9cLMdxsq4ce7GTouXX49WuaOEyluXvvZnRc1GcYagwJvz9-eWXqoWKVoTRusxFVASgeVSsMAhe0rpmj4sVgBAlIQ2cFM9SuoVMAONPixNMswnFdFW4azMuTs02eBQGNCxT8DZZjzob0s7PG5NsQvm-XpKKdpnQnYmz1dY5G2xvEup2SKHf1uss8Yt2RkWk3ZJmE8t5tzVoNN68Qz_W1_h58WRQLpkXx_O0uFlffr_4VF59_fj54vyq1JTCXPIBmkYoU4sORE1JT0UjzNByrLSAvhkaxmvGGGmBamh5z1VrmCZdI4iqh74-LT4cfLdLN5leGz9H5eQ22knFnQzKyn9fvN3IMdzJJjdmkPVvj_oYfi4mzXKySRvnlDdhSRIzLLAQJIOv_wNvwxJ93k0SoILmKdsMtQdIx5BSNMPfQTDIfZoypylbkFTu09wXIfdpZuXLh3vc647xZeDNEVBJKzdE5bVND7hWMM5F5l4duI0dN79sNFKlSSoz3bfNzNmBGVSQaozZ5-YbAVznX9RwzET9B2Y8vfk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205953667</pqid></control><display><type>article</type><title>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Flaherty, J.E ; Woloshuk, C.P</creator><creatorcontrib>Flaherty, J.E ; Woloshuk, C.P</creatorcontrib><description>Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.70.5.2653-2659.2004</identifier><identifier>PMID: 15128515</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>amino acid sequences ; Biological and medical sciences ; biosynthesis ; complementary DNA ; Corn ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; fumonisin B1 ; Fumonisins - metabolism ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fusarium - genetics ; Fusarium - metabolism ; Fusarium verticillioides ; Gene Deletion ; Gene expression ; gene expression regulation ; Gene Expression Regulation, Fungal ; Gibberella fujikuroi ; messenger RNA ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Mutation ; Mycology ; nucleotide sequences ; plant pathogenic fungi ; Proteins ; regulator genes ; seeds ; Sequence Analysis, DNA ; Toxins ; transcription (genetics) ; transcription factors ; Zea mays ; Zea mays - microbiology ; ZFR1 gene ; Zinc Fingers</subject><ispartof>Applied and Environmental Microbiology, 2004-05, Vol.70 (5), p.2653-2659</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 2004</rights><rights>Copyright © 2004, American Society for Microbiology 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</citedby><cites>FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC404460/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC404460/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,3190,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15796889$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15128515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flaherty, J.E</creatorcontrib><creatorcontrib>Woloshuk, C.P</creatorcontrib><title>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</description><subject>amino acid sequences</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>complementary DNA</subject><subject>Corn</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>fumonisin B1</subject><subject>Fumonisins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fusarium - genetics</subject><subject>Fusarium - metabolism</subject><subject>Fusarium verticillioides</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gibberella fujikuroi</subject><subject>messenger RNA</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Mycology</subject><subject>nucleotide sequences</subject><subject>plant pathogenic fungi</subject><subject>Proteins</subject><subject>regulator genes</subject><subject>seeds</subject><subject>Sequence Analysis, DNA</subject><subject>Toxins</subject><subject>transcription (genetics)</subject><subject>transcription factors</subject><subject>Zea mays</subject><subject>Zea mays - microbiology</subject><subject>ZFR1 gene</subject><subject>Zinc Fingers</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkkFv1DAQhSMEokvhL1CDBCcSxk7s2AcOVdUFpCKkQi9cLMdxsq4ce7GTouXX49WuaOEyluXvvZnRc1GcYagwJvz9-eWXqoWKVoTRusxFVASgeVSsMAhe0rpmj4sVgBAlIQ2cFM9SuoVMAONPixNMswnFdFW4azMuTs02eBQGNCxT8DZZjzob0s7PG5NsQvm-XpKKdpnQnYmz1dY5G2xvEup2SKHf1uss8Yt2RkWk3ZJmE8t5tzVoNN68Qz_W1_h58WRQLpkXx_O0uFlffr_4VF59_fj54vyq1JTCXPIBmkYoU4sORE1JT0UjzNByrLSAvhkaxmvGGGmBamh5z1VrmCZdI4iqh74-LT4cfLdLN5leGz9H5eQ22knFnQzKyn9fvN3IMdzJJjdmkPVvj_oYfi4mzXKySRvnlDdhSRIzLLAQJIOv_wNvwxJ93k0SoILmKdsMtQdIx5BSNMPfQTDIfZoypylbkFTu09wXIfdpZuXLh3vc647xZeDNEVBJKzdE5bVND7hWMM5F5l4duI0dN79sNFKlSSoz3bfNzNmBGVSQaozZ5-YbAVznX9RwzET9B2Y8vfk</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Flaherty, J.E</creator><creator>Woloshuk, C.P</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20040501</creationdate><title>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</title><author>Flaherty, J.E ; Woloshuk, C.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>amino acid sequences</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>complementary DNA</topic><topic>Corn</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>fumonisin B1</topic><topic>Fumonisins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fusarium - genetics</topic><topic>Fusarium - metabolism</topic><topic>Fusarium verticillioides</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gibberella fujikuroi</topic><topic>messenger RNA</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Mycology</topic><topic>nucleotide sequences</topic><topic>plant pathogenic fungi</topic><topic>Proteins</topic><topic>regulator genes</topic><topic>seeds</topic><topic>Sequence Analysis, DNA</topic><topic>Toxins</topic><topic>transcription (genetics)</topic><topic>transcription factors</topic><topic>Zea mays</topic><topic>Zea mays - microbiology</topic><topic>ZFR1 gene</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flaherty, J.E</creatorcontrib><creatorcontrib>Woloshuk, C.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flaherty, J.E</au><au>Woloshuk, C.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>70</volume><issue>5</issue><spage>2653</spage><epage>2659</epage><pages>2653-2659</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15128515</pmid><doi>10.1128/AEM.70.5.2653-2659.2004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0099-2240
ispartof Applied and Environmental Microbiology, 2004-05, Vol.70 (5), p.2653-2659
issn 0099-2240
1098-5336
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_404460
source American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection
subjects amino acid sequences
Biological and medical sciences
biosynthesis
complementary DNA
Corn
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
fumonisin B1
Fumonisins - metabolism
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fusarium - genetics
Fusarium - metabolism
Fusarium verticillioides
Gene Deletion
Gene expression
gene expression regulation
Gene Expression Regulation, Fungal
Gibberella fujikuroi
messenger RNA
Microbiology
Molecular Sequence Data
Multigene Family
Mutation
Mycology
nucleotide sequences
plant pathogenic fungi
Proteins
regulator genes
seeds
Sequence Analysis, DNA
Toxins
transcription (genetics)
transcription factors
Zea mays
Zea mays - microbiology
ZFR1 gene
Zinc Fingers
title Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T23%3A18%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulation%20of%20fumonisin%20biosynthesis%20in%20Fusarium%20verticillioides%20by%20a%20zinc%20binuclear%20cluster-type%20gene,%20ZFR1&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Flaherty,%20J.E&rft.date=2004-05-01&rft.volume=70&rft.issue=5&rft.spage=2653&rft.epage=2659&rft.pages=2653-2659&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.70.5.2653-2659.2004&rft_dat=%3Cproquest_pubme%3E16191992%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205953667&rft_id=info:pmid/15128515&rfr_iscdi=true