Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1
Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1...
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description | Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function. |
doi_str_mv | 10.1128/AEM.70.5.2653-2659.2004 |
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In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.70.5.2653-2659.2004</identifier><identifier>PMID: 15128515</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>amino acid sequences ; Biological and medical sciences ; biosynthesis ; complementary DNA ; Corn ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; fumonisin B1 ; Fumonisins - metabolism ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fusarium - genetics ; Fusarium - metabolism ; Fusarium verticillioides ; Gene Deletion ; Gene expression ; gene expression regulation ; Gene Expression Regulation, Fungal ; Gibberella fujikuroi ; messenger RNA ; Microbiology ; Molecular Sequence Data ; Multigene Family ; Mutation ; Mycology ; nucleotide sequences ; plant pathogenic fungi ; Proteins ; regulator genes ; seeds ; Sequence Analysis, DNA ; Toxins ; transcription (genetics) ; transcription factors ; Zea mays ; Zea mays - microbiology ; ZFR1 gene ; Zinc Fingers</subject><ispartof>Applied and Environmental Microbiology, 2004-05, Vol.70 (5), p.2653-2659</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 2004</rights><rights>Copyright © 2004, American Society for Microbiology 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</citedby><cites>FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC404460/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC404460/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,3190,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15796889$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15128515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flaherty, J.E</creatorcontrib><creatorcontrib>Woloshuk, C.P</creatorcontrib><title>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</description><subject>amino acid sequences</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>complementary DNA</subject><subject>Corn</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>fumonisin B1</subject><subject>Fumonisins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fusarium - genetics</subject><subject>Fusarium - metabolism</subject><subject>Fusarium verticillioides</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gibberella fujikuroi</subject><subject>messenger RNA</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Mycology</subject><subject>nucleotide sequences</subject><subject>plant pathogenic fungi</subject><subject>Proteins</subject><subject>regulator genes</subject><subject>seeds</subject><subject>Sequence Analysis, DNA</subject><subject>Toxins</subject><subject>transcription (genetics)</subject><subject>transcription factors</subject><subject>Zea mays</subject><subject>Zea mays - microbiology</subject><subject>ZFR1 gene</subject><subject>Zinc Fingers</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkkFv1DAQhSMEokvhL1CDBCcSxk7s2AcOVdUFpCKkQi9cLMdxsq4ce7GTouXX49WuaOEyluXvvZnRc1GcYagwJvz9-eWXqoWKVoTRusxFVASgeVSsMAhe0rpmj4sVgBAlIQ2cFM9SuoVMAONPixNMswnFdFW4azMuTs02eBQGNCxT8DZZjzob0s7PG5NsQvm-XpKKdpnQnYmz1dY5G2xvEup2SKHf1uss8Yt2RkWk3ZJmE8t5tzVoNN68Qz_W1_h58WRQLpkXx_O0uFlffr_4VF59_fj54vyq1JTCXPIBmkYoU4sORE1JT0UjzNByrLSAvhkaxmvGGGmBamh5z1VrmCZdI4iqh74-LT4cfLdLN5leGz9H5eQ22knFnQzKyn9fvN3IMdzJJjdmkPVvj_oYfi4mzXKySRvnlDdhSRIzLLAQJIOv_wNvwxJ93k0SoILmKdsMtQdIx5BSNMPfQTDIfZoypylbkFTu09wXIfdpZuXLh3vc647xZeDNEVBJKzdE5bVND7hWMM5F5l4duI0dN79sNFKlSSoz3bfNzNmBGVSQaozZ5-YbAVznX9RwzET9B2Y8vfk</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Flaherty, J.E</creator><creator>Woloshuk, C.P</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20040501</creationdate><title>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</title><author>Flaherty, J.E ; Woloshuk, C.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-8f0449ae39b09352d5949ef781ac90d4f46836662705c078d8a7e6c2b492a3fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>amino acid sequences</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>complementary DNA</topic><topic>Corn</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>fumonisin B1</topic><topic>Fumonisins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fusarium - genetics</topic><topic>Fusarium - metabolism</topic><topic>Fusarium verticillioides</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Gibberella fujikuroi</topic><topic>messenger RNA</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Mycology</topic><topic>nucleotide sequences</topic><topic>plant pathogenic fungi</topic><topic>Proteins</topic><topic>regulator genes</topic><topic>seeds</topic><topic>Sequence Analysis, DNA</topic><topic>Toxins</topic><topic>transcription (genetics)</topic><topic>transcription factors</topic><topic>Zea mays</topic><topic>Zea mays - microbiology</topic><topic>ZFR1 gene</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flaherty, J.E</creatorcontrib><creatorcontrib>Woloshuk, C.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flaherty, J.E</au><au>Woloshuk, C.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>70</volume><issue>5</issue><spage>2653</spage><epage>2659</epage><pages>2653-2659</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Fusarium verticillioides, a pathogen of maize, produces a class of mycotoxins called fumonisins in infected kernels. In this study, a candidate regulatory gene, ZFR1, was identified in an expressed sequence tag library enriched for transcripts expressed by F. verticillioides during fumonisin B1 (FB1) biosynthesis. ZFR1 deletion mutants exhibited normal growth and development on maize kernels, but fumonisin production was reduced to less than 10% of that of the wild-type strain. ZFR1 encodes a putative protein of 705 amino acids with sequence similarity to the Zn(II)2Cys6 binuclear cluster family that are regulators of both primary and secondary metabolism in fungi. Expression of ZFR1 in colonized germ and degermed kernel tissues correlated with FB1 levels. Overexpression of ZFR1 in zfr1 mutants restored FB1 production to wild-type levels; however, FB1 was not restored in an fcc1 (Fusarium C-type cyclin) mutant by overexpression of ZFR1. The results of this study indicate that ZFR1 is a positive regulator of FB1 biosynthesis in F. verticillioides and suggest that FCC1 is required for ZFR1 function.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15128515</pmid><doi>10.1128/AEM.70.5.2653-2659.2004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | amino acid sequences Biological and medical sciences biosynthesis complementary DNA Corn DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism fumonisin B1 Fumonisins - metabolism Fundamental and applied biological sciences. Psychology fungal diseases of plants Fungal Proteins - genetics Fungal Proteins - metabolism Fusarium - genetics Fusarium - metabolism Fusarium verticillioides Gene Deletion Gene expression gene expression regulation Gene Expression Regulation, Fungal Gibberella fujikuroi messenger RNA Microbiology Molecular Sequence Data Multigene Family Mutation Mycology nucleotide sequences plant pathogenic fungi Proteins regulator genes seeds Sequence Analysis, DNA Toxins transcription (genetics) transcription factors Zea mays Zea mays - microbiology ZFR1 gene Zinc Fingers |
title | Regulation of fumonisin biosynthesis in Fusarium verticillioides by a zinc binuclear cluster-type gene, ZFR1 |
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