Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa
The bronchial microbiome in severe COPD during stability and exacerbation in patients chronically colonised by Pseudomonas aeruginosa (PA), has not been defined. Our objective was to determine the characteristics of the bronchial microbiome of severe COPD patients colonised and not colonised by P. a...
Gespeichert in:
Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2014-07, Vol.33 (7), p.1101-1111 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The bronchial microbiome in severe COPD during stability and exacerbation in patients chronically colonised by
Pseudomonas aeruginosa
(PA), has not been defined. Our objective was to determine the characteristics of the bronchial microbiome of severe COPD patients colonised and not colonised by
P. aeruginosa
and its changes during exacerbation. COPD patients with severe disease and frequent exacerbations were categorised according to chronic colonisation by
P. aeruginosa
. Sputum samples were obtained in stability and exacerbation, cultured, and analysed by 16S rRNA gene amplification and pyrosequencing. Sixteen patients were included, 5 of them showing chronic colonisation by
P. aeruginosa. Pseudomonas
genus had significantly higher relative abundance in stable colonised patients (
p
= 0.019), but no significant differences in biodiversity parameters were found between the two groups (Shannon, 3 (2–4) vs 3 (2–3),
p
= 0.699; Chao1, 124 (77–159) vs 140 (115–163),
p
= 0.364). In PA-colonised patients bronchial microbiome changed to a microbiome similar to non-PA-colonised patients during exacerbations. An increase in the relative abundance over 20 % during exacerbation was found for
Streptococcus
,
Pseudomonas
,
Moraxella
,
Haemophilus
,
Neisseria
,
Achromobacter
and
Corynebacterium
genera, which include recognised potentially pathogenic microorganisms, in 13 patients colonised and not colonised by
P. aeruginosa
with paired samples. These increases were not identified by culture in 5 out of 13 participants (38.5 %). Stable COPD patients with severe disease and PA-colonised showed a similar biodiversity to non-PA-colonised patients, with a higher relative abundance of
Pseudomonas
genus in bronchial secretions. Exacerbation in severe COPD patients showed the same microbial pattern, independently of previous colonisation by
P. aeruginosa
. |
---|---|
ISSN: | 0934-9723 1435-4373 1435-4373 |
DOI: | 10.1007/s10096-013-2044-0 |