Molecular and functional consequences of mutations in the central helix of cardiac troponin C

•D87A/D88A and E94A/E95A/E96A mutations were introduced into the central helix of cTnC.•Central helix mutations decreased affinity of Ca2+ saturated cTnC for cTnI128–180.•Central helix mutations desensitized the cTn complex and thin filaments to Ca2+.•Central helix mutations decreased the Ca2+ sensi...

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