Bioinformatics Analysis of Alternative Polyadenylation in Green Alga Chlamydomonas reinhardtii Using Transcriptome Sequences from Three Different Sequencing Platforms

Abstract Messenger RNA 3′-end formation is an essential posttranscriptional processing step for most eukaryotic genes. Different from plants and animals where AAUAAA and its variants routinely are found as the main poly(A) signal, Chlamydomonas reinhardtii uses UGUAA as the major poly(A) signal. The...

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Veröffentlicht in:G3 : genes - genomes - genetics 2014-05, Vol.4 (5), p.871-883
Hauptverfasser: Zhao, Zhixin, Wu, Xiaohui, Kumar, Praveen Kumar Raj, Dong, Min, Ji, Guoli, Li, Qingshun Quinn, Liang, Chun
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container_title G3 : genes - genomes - genetics
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creator Zhao, Zhixin
Wu, Xiaohui
Kumar, Praveen Kumar Raj
Dong, Min
Ji, Guoli
Li, Qingshun Quinn
Liang, Chun
description Abstract Messenger RNA 3′-end formation is an essential posttranscriptional processing step for most eukaryotic genes. Different from plants and animals where AAUAAA and its variants routinely are found as the main poly(A) signal, Chlamydomonas reinhardtii uses UGUAA as the major poly(A) signal. The advance of sequencing technology provides an enormous amount of sequencing data for us to explore the variations of poly(A) signals, alternative polyadenylation (APA), and its relationship with splicing in this algal species. Through genome-wide analysis of poly(A) sites in C. reinhardtii, we identified a large number of poly(A) sites: 21,041 from Sanger expressed sequence tags, 88,184 from 454, and 195,266 from Illumina sequence reads. In comparison with previous collections, more new poly(A) sites are found in coding sequences and intron and intergenic regions by deep-sequencing. Interestingly, G-rich signals are particularly abundant in intron and intergenic regions. The prevalence of different poly(A) signals between coding sequences and a 3′-untranslated region implies potentially different polyadenylation mechanisms. Our data suggest that the APA occurs in about 68% of C. reinhardtii genes. Using Gene Ontolgy analysis, we found most of the APA genes are involved in RNA regulation and metabolic process, protein synthesis, hydrolase, and ligase activities. Moreover, intronic poly(A) sites are more abundant in constitutively spliced introns than retained introns, suggesting an interplay between polyadenylation and splicing. Our results support that APA, as in higher eukaryotes, may play significant roles in increasing transcriptome diversity and gene expression regulation in this algal species. Our datasets also provide useful information for accurate annotation of transcript ends in C. reinhardtii.
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Different from plants and animals where AAUAAA and its variants routinely are found as the main poly(A) signal, Chlamydomonas reinhardtii uses UGUAA as the major poly(A) signal. The advance of sequencing technology provides an enormous amount of sequencing data for us to explore the variations of poly(A) signals, alternative polyadenylation (APA), and its relationship with splicing in this algal species. Through genome-wide analysis of poly(A) sites in C. reinhardtii, we identified a large number of poly(A) sites: 21,041 from Sanger expressed sequence tags, 88,184 from 454, and 195,266 from Illumina sequence reads. In comparison with previous collections, more new poly(A) sites are found in coding sequences and intron and intergenic regions by deep-sequencing. Interestingly, G-rich signals are particularly abundant in intron and intergenic regions. The prevalence of different poly(A) signals between coding sequences and a 3′-untranslated region implies potentially different polyadenylation mechanisms. Our data suggest that the APA occurs in about 68% of C. reinhardtii genes. Using Gene Ontolgy analysis, we found most of the APA genes are involved in RNA regulation and metabolic process, protein synthesis, hydrolase, and ligase activities. Moreover, intronic poly(A) sites are more abundant in constitutively spliced introns than retained introns, suggesting an interplay between polyadenylation and splicing. Our results support that APA, as in higher eukaryotes, may play significant roles in increasing transcriptome diversity and gene expression regulation in this algal species. 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The prevalence of different poly(A) signals between coding sequences and a 3′-untranslated region implies potentially different polyadenylation mechanisms. Our data suggest that the APA occurs in about 68% of C. reinhardtii genes. Using Gene Ontolgy analysis, we found most of the APA genes are involved in RNA regulation and metabolic process, protein synthesis, hydrolase, and ligase activities. Moreover, intronic poly(A) sites are more abundant in constitutively spliced introns than retained introns, suggesting an interplay between polyadenylation and splicing. Our results support that APA, as in higher eukaryotes, may play significant roles in increasing transcriptome diversity and gene expression regulation in this algal species. Our datasets also provide useful information for accurate annotation of transcript ends in C. reinhardtii.</description><subject>Alternative Splicing</subject><subject>Chlamydomonas reinhardtii - genetics</subject><subject>Computational Biology</subject><subject>Datasets as Topic</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genome, Plant</subject><subject>High-Throughput Nucleotide Sequencing - methods</subject><subject>Introns</subject><subject>Investigations</subject><subject>Open Reading Frames</subject><subject>Poly A</subject><subject>Polyadenylation - genetics</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Transcription, Genetic</subject><subject>Transcriptome</subject><issn>2160-1836</issn><issn>2160-1836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFu3CAUtKpGSZTmmGvFsRdvAWMwl0rbTZtWitRI2ZwRix9eKgxb8EbyD_U7y2qTND2Vy4P3hpl5mqq6InhB2oZ9HJoFIWyBCaZMvqnOKeG4Jl3D3766n1WXOf_E5bQt54yfVmeUccpp151Xvz-76IKNadSTMxktg_ZzdhlFi5Z-ghRK_xHQXfSz7iHMvrxjQC6gmwQQCmjQaLX1epz7OMagM0rgwlanfnIOPWQXBrROOmST3G6KI6B7-LWHYCAjm-KI1ttChK6dtZAgTM_jw7-7onbwlt9VJ1b7DJdP9aJ6-PplvfpW3_64-b5a3taGSTzVxHLRg2SWNsL2rYBeYCtBCoyZEKK3G0YkEXZjbNmfcU4a3hlsGEjLjSbNRfXpyLvbb0boTfGTtFe75EadZhW1U_9OgtuqIT4qhmnLOl4IPjwRpFjWyJMaXTbgvQ4Q91mRlnJCOylkgdZHqEkx5wT2RYZgdYhXDY0q8apjvAX__rW3F_RzmH-14373H64_Axuxow</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Zhao, Zhixin</creator><creator>Wu, Xiaohui</creator><creator>Kumar, Praveen Kumar Raj</creator><creator>Dong, Min</creator><creator>Ji, Guoli</creator><creator>Li, Qingshun Quinn</creator><creator>Liang, Chun</creator><general>Oxford University Press</general><general>Genetics Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>Bioinformatics Analysis of Alternative Polyadenylation in Green Alga Chlamydomonas reinhardtii Using Transcriptome Sequences from Three Different Sequencing Platforms</title><author>Zhao, Zhixin ; Wu, Xiaohui ; Kumar, Praveen Kumar Raj ; Dong, Min ; Ji, Guoli ; Li, Qingshun Quinn ; Liang, Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-1f67de94f237fd57ed70f9e97004777dfb41917fbcf2624661368c0c4e9f6ca13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alternative Splicing</topic><topic>Chlamydomonas reinhardtii - genetics</topic><topic>Computational Biology</topic><topic>Datasets as Topic</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genome, Plant</topic><topic>High-Throughput Nucleotide Sequencing - methods</topic><topic>Introns</topic><topic>Investigations</topic><topic>Open Reading Frames</topic><topic>Poly A</topic><topic>Polyadenylation - genetics</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Transcription, Genetic</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Zhixin</creatorcontrib><creatorcontrib>Wu, Xiaohui</creatorcontrib><creatorcontrib>Kumar, Praveen Kumar Raj</creatorcontrib><creatorcontrib>Dong, Min</creatorcontrib><creatorcontrib>Ji, Guoli</creatorcontrib><creatorcontrib>Li, Qingshun Quinn</creatorcontrib><creatorcontrib>Liang, Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>G3 : genes - genomes - genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Zhixin</au><au>Wu, Xiaohui</au><au>Kumar, Praveen Kumar Raj</au><au>Dong, Min</au><au>Ji, Guoli</au><au>Li, Qingshun Quinn</au><au>Liang, Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioinformatics Analysis of Alternative Polyadenylation in Green Alga Chlamydomonas reinhardtii Using Transcriptome Sequences from Three Different Sequencing Platforms</atitle><jtitle>G3 : genes - genomes - genetics</jtitle><addtitle>G3 (Bethesda)</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>4</volume><issue>5</issue><spage>871</spage><epage>883</epage><pages>871-883</pages><issn>2160-1836</issn><eissn>2160-1836</eissn><abstract>Abstract Messenger RNA 3′-end formation is an essential posttranscriptional processing step for most eukaryotic genes. 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The prevalence of different poly(A) signals between coding sequences and a 3′-untranslated region implies potentially different polyadenylation mechanisms. Our data suggest that the APA occurs in about 68% of C. reinhardtii genes. Using Gene Ontolgy analysis, we found most of the APA genes are involved in RNA regulation and metabolic process, protein synthesis, hydrolase, and ligase activities. Moreover, intronic poly(A) sites are more abundant in constitutively spliced introns than retained introns, suggesting an interplay between polyadenylation and splicing. Our results support that APA, as in higher eukaryotes, may play significant roles in increasing transcriptome diversity and gene expression regulation in this algal species. Our datasets also provide useful information for accurate annotation of transcript ends in C. reinhardtii.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>24626288</pmid><doi>10.1534/g3.114.010249</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record>
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subjects Alternative Splicing
Chlamydomonas reinhardtii - genetics
Computational Biology
Datasets as Topic
Gene Expression Regulation, Plant
Genome, Plant
High-Throughput Nucleotide Sequencing - methods
Introns
Investigations
Open Reading Frames
Poly A
Polyadenylation - genetics
Polymorphism, Single Nucleotide
Transcription, Genetic
Transcriptome
title Bioinformatics Analysis of Alternative Polyadenylation in Green Alga Chlamydomonas reinhardtii Using Transcriptome Sequences from Three Different Sequencing Platforms
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