Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract of Muntingia calabura L. Leaves
Muntingia calabura L. is a tropical plant species that belongs to the Elaeocarpaceae family. The present study is aimed at determining the hepatoprotective activity of methanol extract of M. calabura leaves (MEMC) using two models of liver injury in rats. Rats were divided into five groups n=6 and r...
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| creator | Mahmood, N. D. Mamat, S. S. Kamisan, F. H. Yahya, F. Kamarolzaman, M. F. F. Nasir, N. Mohtarrudin, N. Tohid, S. F. Md Zakaria, Zainul Amiruddin |
| description | Muntingia calabura L. is a tropical plant species that belongs to the Elaeocarpaceae family. The present study is aimed at determining the hepatoprotective activity of methanol extract of M. calabura leaves (MEMC) using two models of liver injury in rats. Rats were divided into five groups n=6 and received 10% DMSO (negative control), 50 mg/kg N-acetylcysteine (NAC; positive control), or MEMC (50, 250, and 500 mg/kg) orally once daily for 7 days and on the 8th day were subjected to the hepatotoxic induction using paracetamol (PCM). The blood and liver tissues were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2,2-diphenyl-1-picrylhydrazyl-(DPPH) and superoxide anion-radical scavenging assays. At the same time, oxygen radical antioxidant capacity (ORAC) and total phenolic content were also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of hepatic structure was observed in group pretreated with N-acetylcysteine and MEMC. Hepatotoxic rats pretreated with NAC or MEMC exhibited significant decrease P |
| doi_str_mv | 10.1155/2014/695678 |
| format | Article |
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Leaves</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Mahmood, N. D. ; Mamat, S. S. ; Kamisan, F. H. ; Yahya, F. ; Kamarolzaman, M. F. F. ; Nasir, N. ; Mohtarrudin, N. ; Tohid, S. F. Md ; Zakaria, Zainul Amiruddin</creator><contributor>Reddy, Aramati B.</contributor><creatorcontrib>Mahmood, N. D. ; Mamat, S. S. ; Kamisan, F. H. ; Yahya, F. ; Kamarolzaman, M. F. F. ; Nasir, N. ; Mohtarrudin, N. ; Tohid, S. F. Md ; Zakaria, Zainul Amiruddin ; Reddy, Aramati B.</creatorcontrib><description>Muntingia calabura L. is a tropical plant species that belongs to the Elaeocarpaceae family. The present study is aimed at determining the hepatoprotective activity of methanol extract of M. calabura leaves (MEMC) using two models of liver injury in rats. Rats were divided into five groups n=6 and received 10% DMSO (negative control), 50 mg/kg N-acetylcysteine (NAC; positive control), or MEMC (50, 250, and 500 mg/kg) orally once daily for 7 days and on the 8th day were subjected to the hepatotoxic induction using paracetamol (PCM). The blood and liver tissues were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2,2-diphenyl-1-picrylhydrazyl-(DPPH) and superoxide anion-radical scavenging assays. At the same time, oxygen radical antioxidant capacity (ORAC) and total phenolic content were also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of hepatic structure was observed in group pretreated with N-acetylcysteine and MEMC. Hepatotoxic rats pretreated with NAC or MEMC exhibited significant decrease P<0.05 in ALT and AST enzymes level. Moreover, the extract also exhibited good antioxidant activity. In conclusion, MEMC exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and, thus warrants further investigations.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/695678</identifier><identifier>PMID: 24868543</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Acetaminophen ; Acetaminophen - toxicity ; Active Transport, Cell Nucleus ; AMP-Activated Protein Kinases - metabolism ; Analgesics ; Animals ; Antioxidants ; Cell Nucleus - metabolism ; Dinoprostone - metabolism ; Down-Regulation ; Drugs ; Elaeocarpaceae ; Free radicals ; Health aspects ; Health sciences ; Humans ; Immunohistochemistry ; Inflammation ; Interleukin-6 - metabolism ; Laboratory animals ; Lipid peroxidation ; Lipopolysaccharides - metabolism ; Liver - drug effects ; Liver Failure - chemically induced ; Liver Failure - drug therapy ; Medicine ; Medicine, Botanic ; Medicine, Herbal ; Methanol ; Mice ; Microglia - metabolism ; Muntingia calabura ; NF-kappa B - metabolism ; Nitric Oxide - metabolism ; Plant Extracts - therapeutic use ; Plant Leaves - chemistry ; Rats ; Sasa - chemistry ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-10</ispartof><rights>Copyright © 2014 N. D. Mahmood et al.</rights><rights>COPYRIGHT 2014 John Wiley & Sons, Inc.</rights><rights>Copyright © 2014 N. D. Mahmood et al. N. D. Mahmood et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 N. D. Mahmood et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-b3157d466d1be5aba156a60ebd5f1e4149ad1a78e83158f0cfc3540b08352b793</citedby><cites>FETCH-LOGICAL-c490t-b3157d466d1be5aba156a60ebd5f1e4149ad1a78e83158f0cfc3540b08352b793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017787/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017787/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24868543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Reddy, Aramati B.</contributor><creatorcontrib>Mahmood, N. D.</creatorcontrib><creatorcontrib>Mamat, S. S.</creatorcontrib><creatorcontrib>Kamisan, F. H.</creatorcontrib><creatorcontrib>Yahya, F.</creatorcontrib><creatorcontrib>Kamarolzaman, M. F. F.</creatorcontrib><creatorcontrib>Nasir, N.</creatorcontrib><creatorcontrib>Mohtarrudin, N.</creatorcontrib><creatorcontrib>Tohid, S. F. Md</creatorcontrib><creatorcontrib>Zakaria, Zainul Amiruddin</creatorcontrib><title>Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract of Muntingia calabura L. Leaves</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Muntingia calabura L. is a tropical plant species that belongs to the Elaeocarpaceae family. The present study is aimed at determining the hepatoprotective activity of methanol extract of M. calabura leaves (MEMC) using two models of liver injury in rats. 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Moreover, the extract also exhibited good antioxidant activity. In conclusion, MEMC exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and, thus warrants further investigations.</description><subject>Acetaminophen</subject><subject>Acetaminophen - toxicity</subject><subject>Active Transport, Cell Nucleus</subject><subject>AMP-Activated Protein Kinases - metabolism</subject><subject>Analgesics</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Cell Nucleus - metabolism</subject><subject>Dinoprostone - metabolism</subject><subject>Down-Regulation</subject><subject>Drugs</subject><subject>Elaeocarpaceae</subject><subject>Free radicals</subject><subject>Health aspects</subject><subject>Health sciences</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Interleukin-6 - metabolism</subject><subject>Laboratory animals</subject><subject>Lipid peroxidation</subject><subject>Lipopolysaccharides - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver Failure - chemically induced</subject><subject>Liver Failure - drug therapy</subject><subject>Medicine</subject><subject>Medicine, Botanic</subject><subject>Medicine, Herbal</subject><subject>Methanol</subject><subject>Mice</subject><subject>Microglia - metabolism</subject><subject>Muntingia calabura</subject><subject>NF-kappa B - metabolism</subject><subject>Nitric Oxide - metabolism</subject><subject>Plant Extracts - therapeutic use</subject><subject>Plant Leaves - chemistry</subject><subject>Rats</subject><subject>Sasa - chemistry</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkkFv1DAQRiMEolXpiTPIEhcE2tZOHNu5VFpVLa20CITgbE2cya6rxF5ip3TP_PE6pGyBC77YGj89fWNPlr1k9ISxsjzNKeOnoiqFVE-yw7xgfCEYZ0_356I4yI5DuKFpKSZoJZ5nBzlXQpW8OMx-LnvsrB8gWu-Ib8lnGMBghN53i2vXjAYbcoVbiD76O2ts3BHryBeIpN6RuEGybHrrbIiPio8YN-B8Ry7uUtXEX7XRRevWFoiBDupxALI6ISuEWwwvsmctdAGPH_aj7Nvlxdfzq8Xq04fr8-VqYXhF46IuWCkbLkTDaiyhBlYKEBTrpmwZcsYraBhIhSqBqqWmNUXJaU1VUea1rIqj7Gz2bse6x8agS_E6vR1sD8NOe7D67xtnN3rtbzWnTEolk-Dtg2Dw30cMUfc2GOw6cOjHoFMgoQqZ53lC3_yD3vhxcKm9RHGR0iopH6k1dKita_30YJNUL3kuac5pNeV-P1Nm8CEM2O4jM6qnKdDTFOh5ChL9-s8u9-zvP0_AuxnYWNfAD_sf26sZxoRgC3uYVyxXqrgHgQTDVg</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Mahmood, N. 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D.</au><au>Mamat, S. S.</au><au>Kamisan, F. H.</au><au>Yahya, F.</au><au>Kamarolzaman, M. F. F.</au><au>Nasir, N.</au><au>Mohtarrudin, N.</au><au>Tohid, S. F. Md</au><au>Zakaria, Zainul Amiruddin</au><au>Reddy, Aramati B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract of Muntingia calabura L. Leaves</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Muntingia calabura L. is a tropical plant species that belongs to the Elaeocarpaceae family. The present study is aimed at determining the hepatoprotective activity of methanol extract of M. calabura leaves (MEMC) using two models of liver injury in rats. Rats were divided into five groups n=6 and received 10% DMSO (negative control), 50 mg/kg N-acetylcysteine (NAC; positive control), or MEMC (50, 250, and 500 mg/kg) orally once daily for 7 days and on the 8th day were subjected to the hepatotoxic induction using paracetamol (PCM). The blood and liver tissues were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2,2-diphenyl-1-picrylhydrazyl-(DPPH) and superoxide anion-radical scavenging assays. At the same time, oxygen radical antioxidant capacity (ORAC) and total phenolic content were also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of hepatic structure was observed in group pretreated with N-acetylcysteine and MEMC. Hepatotoxic rats pretreated with NAC or MEMC exhibited significant decrease P<0.05 in ALT and AST enzymes level. Moreover, the extract also exhibited good antioxidant activity. In conclusion, MEMC exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and, thus warrants further investigations.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>24868543</pmid><doi>10.1155/2014/695678</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetaminophen Acetaminophen - toxicity Active Transport, Cell Nucleus AMP-Activated Protein Kinases - metabolism Analgesics Animals Antioxidants Cell Nucleus - metabolism Dinoprostone - metabolism Down-Regulation Drugs Elaeocarpaceae Free radicals Health aspects Health sciences Humans Immunohistochemistry Inflammation Interleukin-6 - metabolism Laboratory animals Lipid peroxidation Lipopolysaccharides - metabolism Liver - drug effects Liver Failure - chemically induced Liver Failure - drug therapy Medicine Medicine, Botanic Medicine, Herbal Methanol Mice Microglia - metabolism Muntingia calabura NF-kappa B - metabolism Nitric Oxide - metabolism Plant Extracts - therapeutic use Plant Leaves - chemistry Rats Sasa - chemistry Tumor Necrosis Factor-alpha - metabolism |
| title | Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract of Muntingia calabura L. Leaves |
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