Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping. Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children. A total of 39 EPEC collected strains were serotyped and the presence of...
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| Veröffentlicht in: | Gastroenterology and hepatology from bed to bench 2012, Vol.5 (2), p.94-99 |
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| container_title | Gastroenterology and hepatology from bed to bench |
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| creator | Nikbin, Vajihe Sadat Alikhani, Mohammad Yousef Shojaei, Hasan Mousavi, Seyed Fazlollah Salmanzadeh-Ahrabi, Siavosh Aslani, Mohammad Mehdi |
| description | We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping.
Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.
A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.
Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.
According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa. |
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Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.
A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.
Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.
According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.</description><identifier>ISSN: 2008-2258</identifier><identifier>EISSN: 2008-4234</identifier><identifier>PMID: 24834207</identifier><language>eng</language><publisher>Iran: Research Institute for Gastroenterology and Liver Diseases</publisher><subject>Original</subject><ispartof>Gastroenterology and hepatology from bed to bench, 2012, Vol.5 (2), p.94-99</ispartof><rights>Copyright © 2012 Research Institute for Gastroenterology and Liver Diseases 2012</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017452/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017452/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24834207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nikbin, Vajihe Sadat</creatorcontrib><creatorcontrib>Alikhani, Mohammad Yousef</creatorcontrib><creatorcontrib>Shojaei, Hasan</creatorcontrib><creatorcontrib>Mousavi, Seyed Fazlollah</creatorcontrib><creatorcontrib>Salmanzadeh-Ahrabi, Siavosh</creatorcontrib><creatorcontrib>Aslani, Mohammad Mehdi</creatorcontrib><title>Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran</title><title>Gastroenterology and hepatology from bed to bench</title><addtitle>Gastroenterol Hepatol Bed Bench</addtitle><description>We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping.
Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.
A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.
Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.
According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.</description><subject>Original</subject><issn>2008-2258</issn><issn>2008-4234</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpVkNFLwzAQxoMoTub-BcmjL4X0kjTpiyBj6mCgiD6Xa5tukTapSSbsv7fiFL2Huw_u4_cdd0IugDGdCeDi9KgBpJ6RRYxvbCrOc6XKczIDobkApi7I5tnWPh1G67bUd3T1tFpSG32PyUTaBT_Q1mIIO4M9RddSjIdhTH7AZBs6Tt24FKl1dB3QXZKzDvtoFsc5J693q5flQ7Z5vF8vbzfZmOs8ZXWBUgluGJN100nFWw2sAMlL3QCTSkNbNoUoJXQMWCu01MiZLEWhZG10yefk5ps77uvBtM10Q8C-GoMdMBwqj7b6v3F2V239RyVYroSECXB9BAT_vjcxVYONjel7dMbvY5VLkKqYjF_Wq79ZvyE_L-SfyPBuLQ</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Nikbin, Vajihe Sadat</creator><creator>Alikhani, Mohammad Yousef</creator><creator>Shojaei, Hasan</creator><creator>Mousavi, Seyed Fazlollah</creator><creator>Salmanzadeh-Ahrabi, Siavosh</creator><creator>Aslani, Mohammad Mehdi</creator><general>Research Institute for Gastroenterology and Liver Diseases</general><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2012</creationdate><title>Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran</title><author>Nikbin, Vajihe Sadat ; Alikhani, Mohammad Yousef ; Shojaei, Hasan ; Mousavi, Seyed Fazlollah ; Salmanzadeh-Ahrabi, Siavosh ; Aslani, Mohammad Mehdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p181t-b6a5743e005bcf573d820625398c205782d9c64952f020d4858a30594675be893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Nikbin, Vajihe Sadat</creatorcontrib><creatorcontrib>Alikhani, Mohammad Yousef</creatorcontrib><creatorcontrib>Shojaei, Hasan</creatorcontrib><creatorcontrib>Mousavi, Seyed Fazlollah</creatorcontrib><creatorcontrib>Salmanzadeh-Ahrabi, Siavosh</creatorcontrib><creatorcontrib>Aslani, Mohammad Mehdi</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Gastroenterology and hepatology from bed to bench</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nikbin, Vajihe Sadat</au><au>Alikhani, Mohammad Yousef</au><au>Shojaei, Hasan</au><au>Mousavi, Seyed Fazlollah</au><au>Salmanzadeh-Ahrabi, Siavosh</au><au>Aslani, Mohammad Mehdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran</atitle><jtitle>Gastroenterology and hepatology from bed to bench</jtitle><addtitle>Gastroenterol Hepatol Bed Bench</addtitle><date>2012</date><risdate>2012</risdate><volume>5</volume><issue>2</issue><spage>94</spage><epage>99</epage><pages>94-99</pages><issn>2008-2258</issn><eissn>2008-4234</eissn><abstract>We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping.
Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children.
A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping.
Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters.
According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.</abstract><cop>Iran</cop><pub>Research Institute for Gastroenterology and Liver Diseases</pub><pmid>24834207</pmid><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Original |
| title | Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran |
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