Use of lectin microarray to differentiate gastric cancer from gastric ulcer
AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lecti...
Gespeichert in:
| Veröffentlicht in: | World journal of gastroenterology : WJG 2014-05, Vol.20 (18), p.5474-5482 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5482 |
|---|---|
| container_issue | 18 |
| container_start_page | 5474 |
| container_title | World journal of gastroenterology : WJG |
| container_volume | 20 |
| creator | Huang, Wei-Li Li, Yang-Guang Lv, Yong-Chen Guan, Xiao-Hui Ji, Hui-Fan Chi, Bao-Rong |
| description | AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lectins were dissolved in buffer,and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized.The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block.Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval.Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody.The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray,and then validated by lectin histochemistry.Data are presented as mean±SD for the indicated number of independent experiments.RESULTS:The glycosylation level of gastric cancer was significantly higher than that in ulcer.In gastric cancer,most of the lectin binders showed positive signals and the intensity of the signals was stronger,whereas the opposite was the case for ulcers.Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin.For MPL and VVA,all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer,especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma.GalNAc bound to MPL showed a significant increase.A statistically significant association between MPL and gastric cancer was observed.As with MPL,there were significant differences in VVA staining between gastric cancer and ulcer.CONCLUSION:Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer,and the lectins MPL and VVA can be used as biomarkers. |
| doi_str_mv | 10.3748/wjg.v20.i18.5474 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4017062</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>90888889504849524956485150</cqvip_id><sourcerecordid>1525764583</sourcerecordid><originalsourceid>FETCH-LOGICAL-c506t-41568b2613bbecb3c5e6bc91094f7a850e54cc6e235b413cbb21822d5d7eeaf23</originalsourceid><addsrcrecordid>eNpVkc1r3DAQxUVoSDab3HsqPvbi7ejLki-FEpqkNNBLchaSduxVsK1E8qbkv4-W3SytQAhGb94M70fIZworroT-9vepX70yWAWqV1IocUIWjNG2ZlrAJ7KgAKpuOVPn5CLnJwDGuWRn5JwJzblWakF-P2asYlcN6OcwVWPwKdqU7Fs1x2odug4TTnOwM1a9zXMKvvJ28piqLsXxWNsOpXRJTjs7ZLw6vEvyePPz4fquvv9z--v6x33tJTRzLahstGMN5c6hd9xLbJxvKbSiU1ZLQCm8b5Bx6QTl3jlGNWNruVaItmN8Sb7vfZ-3bsS1LwsmO5jnFEab3ky0wfz_M4WN6eOrEUAVNDuDrweDFF-2mGczhuxxGOyEcZsNlUyqRsgS0pLAXlpyyTlhdxxDwewYmMLAFAamMDA7BqXly7_rHRs-Qi8CfvDcxKl_CVN_1LSgd6eVILRoJSu3EVpSCfwdQNCUmg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1525764583</pqid></control><display><type>article</type><title>Use of lectin microarray to differentiate gastric cancer from gastric ulcer</title><source>PubMed (Medline)</source><source>MEDLINE</source><source>Baishideng "World Journal of" online journals</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Huang, Wei-Li ; Li, Yang-Guang ; Lv, Yong-Chen ; Guan, Xiao-Hui ; Ji, Hui-Fan ; Chi, Bao-Rong</creator><creatorcontrib>Huang, Wei-Li ; Li, Yang-Guang ; Lv, Yong-Chen ; Guan, Xiao-Hui ; Ji, Hui-Fan ; Chi, Bao-Rong</creatorcontrib><description>AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lectins were dissolved in buffer,and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized.The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block.Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval.Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody.The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray,and then validated by lectin histochemistry.Data are presented as mean±SD for the indicated number of independent experiments.RESULTS:The glycosylation level of gastric cancer was significantly higher than that in ulcer.In gastric cancer,most of the lectin binders showed positive signals and the intensity of the signals was stronger,whereas the opposite was the case for ulcers.Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin.For MPL and VVA,all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer,especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma.GalNAc bound to MPL showed a significant increase.A statistically significant association between MPL and gastric cancer was observed.As with MPL,there were significant differences in VVA staining between gastric cancer and ulcer.CONCLUSION:Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer,and the lectins MPL and VVA can be used as biomarkers.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v20.i18.5474</identifier><identifier>PMID: 24833877</identifier><language>eng</language><publisher>United States: Baishideng Publishing Group Co., Limited</publisher><subject>Adult ; Aged ; Biomarkers, Tumor - analysis ; Biotinylation ; cancer ; Diagnosis, Differential ; Feasibility Studies ; Female ; Gastric ; Glycoproteins - analysis ; Glycosylation ; Humans ; Immunoenzyme Techniques ; Lec ; Lectin ; Lectins ; Male ; microarray ; Middle Aged ; Original ; Plant Lectins ; Predictive Value of Tests ; Stomach Neoplasms - chemistry ; Stomach Neoplasms - pathology ; Stomach Ulcer - diagnosis ; Stomach Ulcer - metabolism ; Tissue Array Analysis ; ulcer</subject><ispartof>World journal of gastroenterology : WJG, 2014-05, Vol.20 (18), p.5474-5482</ispartof><rights>2014 Baishideng Publishing Group Co., Limited. All rights reserved. 2014</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-41568b2613bbecb3c5e6bc91094f7a850e54cc6e235b413cbb21822d5d7eeaf23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017062/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017062/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24833877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Wei-Li</creatorcontrib><creatorcontrib>Li, Yang-Guang</creatorcontrib><creatorcontrib>Lv, Yong-Chen</creatorcontrib><creatorcontrib>Guan, Xiao-Hui</creatorcontrib><creatorcontrib>Ji, Hui-Fan</creatorcontrib><creatorcontrib>Chi, Bao-Rong</creatorcontrib><title>Use of lectin microarray to differentiate gastric cancer from gastric ulcer</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lectins were dissolved in buffer,and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized.The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block.Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval.Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody.The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray,and then validated by lectin histochemistry.Data are presented as mean±SD for the indicated number of independent experiments.RESULTS:The glycosylation level of gastric cancer was significantly higher than that in ulcer.In gastric cancer,most of the lectin binders showed positive signals and the intensity of the signals was stronger,whereas the opposite was the case for ulcers.Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin.For MPL and VVA,all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer,especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma.GalNAc bound to MPL showed a significant increase.A statistically significant association between MPL and gastric cancer was observed.As with MPL,there were significant differences in VVA staining between gastric cancer and ulcer.CONCLUSION:Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer,and the lectins MPL and VVA can be used as biomarkers.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biotinylation</subject><subject>cancer</subject><subject>Diagnosis, Differential</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Gastric</subject><subject>Glycoproteins - analysis</subject><subject>Glycosylation</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Lec</subject><subject>Lectin</subject><subject>Lectins</subject><subject>Male</subject><subject>microarray</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Plant Lectins</subject><subject>Predictive Value of Tests</subject><subject>Stomach Neoplasms - chemistry</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Ulcer - diagnosis</subject><subject>Stomach Ulcer - metabolism</subject><subject>Tissue Array Analysis</subject><subject>ulcer</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1r3DAQxUVoSDab3HsqPvbi7ejLki-FEpqkNNBLchaSduxVsK1E8qbkv4-W3SytQAhGb94M70fIZworroT-9vepX70yWAWqV1IocUIWjNG2ZlrAJ7KgAKpuOVPn5CLnJwDGuWRn5JwJzblWakF-P2asYlcN6OcwVWPwKdqU7Fs1x2odug4TTnOwM1a9zXMKvvJ28piqLsXxWNsOpXRJTjs7ZLw6vEvyePPz4fquvv9z--v6x33tJTRzLahstGMN5c6hd9xLbJxvKbSiU1ZLQCm8b5Bx6QTl3jlGNWNruVaItmN8Sb7vfZ-3bsS1LwsmO5jnFEab3ky0wfz_M4WN6eOrEUAVNDuDrweDFF-2mGczhuxxGOyEcZsNlUyqRsgS0pLAXlpyyTlhdxxDwewYmMLAFAamMDA7BqXly7_rHRs-Qi8CfvDcxKl_CVN_1LSgd6eVILRoJSu3EVpSCfwdQNCUmg</recordid><startdate>20140514</startdate><enddate>20140514</enddate><creator>Huang, Wei-Li</creator><creator>Li, Yang-Guang</creator><creator>Lv, Yong-Chen</creator><creator>Guan, Xiao-Hui</creator><creator>Ji, Hui-Fan</creator><creator>Chi, Bao-Rong</creator><general>Baishideng Publishing Group Co., Limited</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140514</creationdate><title>Use of lectin microarray to differentiate gastric cancer from gastric ulcer</title><author>Huang, Wei-Li ; Li, Yang-Guang ; Lv, Yong-Chen ; Guan, Xiao-Hui ; Ji, Hui-Fan ; Chi, Bao-Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-41568b2613bbecb3c5e6bc91094f7a850e54cc6e235b413cbb21822d5d7eeaf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biotinylation</topic><topic>cancer</topic><topic>Diagnosis, Differential</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Gastric</topic><topic>Glycoproteins - analysis</topic><topic>Glycosylation</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Lec</topic><topic>Lectin</topic><topic>Lectins</topic><topic>Male</topic><topic>microarray</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Plant Lectins</topic><topic>Predictive Value of Tests</topic><topic>Stomach Neoplasms - chemistry</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Ulcer - diagnosis</topic><topic>Stomach Ulcer - metabolism</topic><topic>Tissue Array Analysis</topic><topic>ulcer</topic><toplevel>online_resources</toplevel><creatorcontrib>Huang, Wei-Li</creatorcontrib><creatorcontrib>Li, Yang-Guang</creatorcontrib><creatorcontrib>Lv, Yong-Chen</creatorcontrib><creatorcontrib>Guan, Xiao-Hui</creatorcontrib><creatorcontrib>Ji, Hui-Fan</creatorcontrib><creatorcontrib>Chi, Bao-Rong</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Wei-Li</au><au>Li, Yang-Guang</au><au>Lv, Yong-Chen</au><au>Guan, Xiao-Hui</au><au>Ji, Hui-Fan</au><au>Chi, Bao-Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of lectin microarray to differentiate gastric cancer from gastric ulcer</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2014-05-14</date><risdate>2014</risdate><volume>20</volume><issue>18</issue><spage>5474</spage><epage>5482</epage><pages>5474-5482</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM:To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.METHODS:Twenty cases of human gastric cancer tissue and 20 cases of human gastric ulcer tissue were collected and processed.Protein was extracted from the frozen tissues and stored.The lectins were dissolved in buffer,and the sugar-binding specificities of lectins and the layout of the lectin microarray were summarized.The median of the effective data points for each lectin was globally normalized to the sum of medians of all effective data points for each lectin in one block.Formalin-fixed paraffin-embedded gastric cancer tissues and their corresponding gastric ulcer tissues were subjected to Ag retrieval.Biotinylated lectin was used as the primary antibody and HRP-streptavidin as the secondary antibody.The glycopatterns of glycoprotein in gastric cancer and gastric ulcer specimens were determined by lectin microarray,and then validated by lectin histochemistry.Data are presented as mean±SD for the indicated number of independent experiments.RESULTS:The glycosylation level of gastric cancer was significantly higher than that in ulcer.In gastric cancer,most of the lectin binders showed positive signals and the intensity of the signals was stronger,whereas the opposite was the case for ulcers.Significant differences in the pathological score of the two lectins were apparent between ulcer and gastric cancer tissues using the same lectin.For MPL and VVA,all types of gastric cancer detected showed stronger staining and a higher positive rate in comparison with ulcer,especially in the case of signet ring cell carcinoma and intra-mucosal carcinoma.GalNAc bound to MPL showed a significant increase.A statistically significant association between MPL and gastric cancer was observed.As with MPL,there were significant differences in VVA staining between gastric cancer and ulcer.CONCLUSION:Lectin microarray can differentiate the different glycopatterns in gastric cancer and gastric ulcer,and the lectins MPL and VVA can be used as biomarkers.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Co., Limited</pub><pmid>24833877</pmid><doi>10.3748/wjg.v20.i18.5474</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2014-05, Vol.20 (18), p.5474-5482 |
| issn | 1007-9327 2219-2840 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4017062 |
| source | PubMed (Medline); MEDLINE; Baishideng "World Journal of" online journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Adult Aged Biomarkers, Tumor - analysis Biotinylation cancer Diagnosis, Differential Feasibility Studies Female Gastric Glycoproteins - analysis Glycosylation Humans Immunoenzyme Techniques Lec Lectin Lectins Male microarray Middle Aged Original Plant Lectins Predictive Value of Tests Stomach Neoplasms - chemistry Stomach Neoplasms - pathology Stomach Ulcer - diagnosis Stomach Ulcer - metabolism Tissue Array Analysis ulcer |
| title | Use of lectin microarray to differentiate gastric cancer from gastric ulcer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T15%3A38%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Use%20of%20lectin%20microarray%20to%20differentiate%20gastric%20cancer%20from%20gastric%20ulcer&rft.jtitle=World%20journal%20of%20gastroenterology%20:%20WJG&rft.au=Huang,%20Wei-Li&rft.date=2014-05-14&rft.volume=20&rft.issue=18&rft.spage=5474&rft.epage=5482&rft.pages=5474-5482&rft.issn=1007-9327&rft.eissn=2219-2840&rft_id=info:doi/10.3748/wjg.v20.i18.5474&rft_dat=%3Cproquest_pubme%3E1525764583%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1525764583&rft_id=info:pmid/24833877&rft_cqvip_id=90888889504849524956485150&rfr_iscdi=true |