Proenkephalin A is expressed in mesodermal lineages during organogenesis
Proenkephalin A (PEA) encodes several neuropeptides with an opioid activity, as well as other peptides with as yet unknown functions. As an initial step toward finding possible roles for PEA gene products in non‐neuronal tissues, we have determined sites of PEA expression during mouse embryonic deve...
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| Veröffentlicht in: | The EMBO journal 1989-10, Vol.8 (10), p.2917-2923 |
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| creator | Keshet, E. Polakiewicz, R.D. Itin, A. Ornoy, A. Rosen, H. |
| description | Proenkephalin A (PEA) encodes several neuropeptides with an opioid activity, as well as other peptides with as yet unknown functions. As an initial step toward finding possible roles for PEA gene products in non‐neuronal tissues, we have determined sites of PEA expression during mouse embryonic development, employing in situ hybridization. We report here the unexpected observation that in addition to its abundance in brain, PEA RNA is expressed in non‐differentiated mesodermal cells of diverse lineages in the process of their development into several adult tissues and organs; it drops to undetectable levels upon terminal differentiation of these tissues. In a particular example of differentiating mesoderm, the developing kidney, the transient expression of PEA mRNA and of its encoded peptide Met‐enkephalin was demonstrated by both in situ and Northern blot hybridizations, as well as by a radioimmunoassay. These findings suggest a novel role for PEA‐derived peptide(s) in mesoderm growth or differentiation during organogenesis. |
| doi_str_mv | 10.1002/j.1460-2075.1989.tb08441.x |
| format | Article |
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As an initial step toward finding possible roles for PEA gene products in non‐neuronal tissues, we have determined sites of PEA expression during mouse embryonic development, employing in situ hybridization. We report here the unexpected observation that in addition to its abundance in brain, PEA RNA is expressed in non‐differentiated mesodermal cells of diverse lineages in the process of their development into several adult tissues and organs; it drops to undetectable levels upon terminal differentiation of these tissues. In a particular example of differentiating mesoderm, the developing kidney, the transient expression of PEA mRNA and of its encoded peptide Met‐enkephalin was demonstrated by both in situ and Northern blot hybridizations, as well as by a radioimmunoassay. These findings suggest a novel role for PEA‐derived peptide(s) in mesoderm growth or differentiation during organogenesis.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1989.tb08441.x</identifier><identifier>PMID: 2583085</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern ; Cell Differentiation ; Enkephalin, Methionine - biosynthesis ; Enkephalins - biosynthesis ; Enkephalins - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gestational Age ; Histocytochemistry ; Kidney - cytology ; Kidney - embryology ; Kidney - metabolism ; Mesoderm - analysis ; Mesoderm - cytology ; Mesoderm - metabolism ; Mice ; Mice, Inbred BALB C ; Molecular and cellular biology ; Molecular genetics ; Morphogenesis ; Protein Precursors - biosynthesis ; Protein Precursors - genetics ; Radioimmunoassay ; Rats ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis</subject><ispartof>The EMBO journal, 1989-10, Vol.8 (10), p.2917-2923</ispartof><rights>1989 European Molecular Biology Organization</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5381-bef84551e4e0c876c93074c67579962503508a68c587fef9d81bb708eaae321d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC401357/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC401357/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6748888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2583085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Keshet, E.</creatorcontrib><creatorcontrib>Polakiewicz, R.D.</creatorcontrib><creatorcontrib>Itin, A.</creatorcontrib><creatorcontrib>Ornoy, A.</creatorcontrib><creatorcontrib>Rosen, H.</creatorcontrib><title>Proenkephalin A is expressed in mesodermal lineages during organogenesis</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>Proenkephalin A (PEA) encodes several neuropeptides with an opioid activity, as well as other peptides with as yet unknown functions. As an initial step toward finding possible roles for PEA gene products in non‐neuronal tissues, we have determined sites of PEA expression during mouse embryonic development, employing in situ hybridization. We report here the unexpected observation that in addition to its abundance in brain, PEA RNA is expressed in non‐differentiated mesodermal cells of diverse lineages in the process of their development into several adult tissues and organs; it drops to undetectable levels upon terminal differentiation of these tissues. In a particular example of differentiating mesoderm, the developing kidney, the transient expression of PEA mRNA and of its encoded peptide Met‐enkephalin was demonstrated by both in situ and Northern blot hybridizations, as well as by a radioimmunoassay. These findings suggest a novel role for PEA‐derived peptide(s) in mesoderm growth or differentiation during organogenesis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell Differentiation</subject><subject>Enkephalin, Methionine - biosynthesis</subject><subject>Enkephalins - biosynthesis</subject><subject>Enkephalins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gestational Age</subject><subject>Histocytochemistry</subject><subject>Kidney - cytology</subject><subject>Kidney - embryology</subject><subject>Kidney - metabolism</subject><subject>Mesoderm - analysis</subject><subject>Mesoderm - cytology</subject><subject>Mesoderm - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Morphogenesis</subject><subject>Protein Precursors - biosynthesis</subject><subject>Protein Precursors - genetics</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUFv0zAYhi0EGmXwE5AihLglfI7t2EHiUKbBNg3BAc6W43zJXBKn2C10_37uGlVwmuaLLb_Pa33WQ8gbCgUFKN-vCsoryEuQoqC1qotNA4pzWuyekMUxekoWUFY051TVz8mLGFcAIJSkJ-SkFIqBEgty8T1M6H_h-sYMzmfLzMUMd-uAMWKbpZsR49RiGM2QJQBNjzFrt8H5PptCb_zUo8fo4kvyrDNDxFfzfkp-fj7_cXaRX3_7cnm2vM6tYIrmDXaKC0GRI1glK1szkNxWUsi6rkoBTIAylbJp0A67ulW0aSQoNAZZSVt2Sj4e3l1vmxFbi34TzKDXwY0m3OrJOP1_4t2N7qc_mgNlQqb-u7kfpt9bjBs9umhxGIzHaRu1rBlLpHoQpIJVtJQigR8OoA1TjAG74zAU9N6XXum9FL2Xove-9OxL71L59b_fOVZnQSl_O-cmWjN0wXjr4hGrJFdpJWx5wP66AW8fMYA-__rp6v7M7gC2S7Px</recordid><startdate>198910</startdate><enddate>198910</enddate><creator>Keshet, E.</creator><creator>Polakiewicz, R.D.</creator><creator>Itin, A.</creator><creator>Ornoy, A.</creator><creator>Rosen, H.</creator><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>198910</creationdate><title>Proenkephalin A is expressed in mesodermal lineages during organogenesis</title><author>Keshet, E. ; Polakiewicz, R.D. ; Itin, A. ; Ornoy, A. ; Rosen, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5381-bef84551e4e0c876c93074c67579962503508a68c587fef9d81bb708eaae321d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell Differentiation</topic><topic>Enkephalin, Methionine - biosynthesis</topic><topic>Enkephalins - biosynthesis</topic><topic>Enkephalins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gestational Age</topic><topic>Histocytochemistry</topic><topic>Kidney - cytology</topic><topic>Kidney - embryology</topic><topic>Kidney - metabolism</topic><topic>Mesoderm - analysis</topic><topic>Mesoderm - cytology</topic><topic>Mesoderm - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Morphogenesis</topic><topic>Protein Precursors - biosynthesis</topic><topic>Protein Precursors - genetics</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keshet, E.</creatorcontrib><creatorcontrib>Polakiewicz, R.D.</creatorcontrib><creatorcontrib>Itin, A.</creatorcontrib><creatorcontrib>Ornoy, A.</creatorcontrib><creatorcontrib>Rosen, H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keshet, E.</au><au>Polakiewicz, R.D.</au><au>Itin, A.</au><au>Ornoy, A.</au><au>Rosen, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proenkephalin A is expressed in mesodermal lineages during organogenesis</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1989-10</date><risdate>1989</risdate><volume>8</volume><issue>10</issue><spage>2917</spage><epage>2923</epage><pages>2917-2923</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>Proenkephalin A (PEA) encodes several neuropeptides with an opioid activity, as well as other peptides with as yet unknown functions. As an initial step toward finding possible roles for PEA gene products in non‐neuronal tissues, we have determined sites of PEA expression during mouse embryonic development, employing in situ hybridization. We report here the unexpected observation that in addition to its abundance in brain, PEA RNA is expressed in non‐differentiated mesodermal cells of diverse lineages in the process of their development into several adult tissues and organs; it drops to undetectable levels upon terminal differentiation of these tissues. In a particular example of differentiating mesoderm, the developing kidney, the transient expression of PEA mRNA and of its encoded peptide Met‐enkephalin was demonstrated by both in situ and Northern blot hybridizations, as well as by a radioimmunoassay. These findings suggest a novel role for PEA‐derived peptide(s) in mesoderm growth or differentiation during organogenesis.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>2583085</pmid><doi>10.1002/j.1460-2075.1989.tb08441.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Blotting, Northern Cell Differentiation Enkephalin, Methionine - biosynthesis Enkephalins - biosynthesis Enkephalins - genetics Fundamental and applied biological sciences. Psychology Gene expression Gestational Age Histocytochemistry Kidney - cytology Kidney - embryology Kidney - metabolism Mesoderm - analysis Mesoderm - cytology Mesoderm - metabolism Mice Mice, Inbred BALB C Molecular and cellular biology Molecular genetics Morphogenesis Protein Precursors - biosynthesis Protein Precursors - genetics Radioimmunoassay Rats RNA, Messenger - analysis RNA, Messenger - biosynthesis |
| title | Proenkephalin A is expressed in mesodermal lineages during organogenesis |
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