Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR

A frequency-modulated fluorescence encoding method was used as a means to increase the number of fluorophores monitored during infrared-mediated polymerase chain reaction. Laser lines at 488 nm and 561 nm were modulated at 73 and 137 Hz, respectively, exciting fluorescence from the dsDNA intercalati...

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Veröffentlicht in:	Analyst (London) 2014-06, Vol.139 (11), p.2695-2701
Hauptverfasser:	Schrell, Adrian M, Roper, Michael G
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Chemical compounds Deoxyribonucleic acid Dyes Fluorescence Fourier Analysis Frequency domains Lasers Multiplexing Reproduction Reverse Transcriptase Polymerase Chain Reaction - methods Spectrometry, Fluorescence
Online-Zugang:	Volltext
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