A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery
Aim： To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity. Methods： Poly （ethylene glycol）-distearoylphosphatidylethanolamine （PEG-DSPE） was incorporated into polymer-lipid hybrid nanoparticles （PLN） to construct a PEG-DSPE modified long circulating PLN （L-PLN）....
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| Veröffentlicht in: | Acta pharmacologica Sinica 2010-04, Vol.31 (4), p.509-514 |
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| container_title | Acta pharmacologica Sinica |
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| creator | Li, Jian He, Ying-zi Li, Wen Shen, Yun-zhen Li, Yu-ru Wang, Yun-feng |
| description | Aim： To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity.
Methods： Poly （ethylene glycol）-distearoylphosphatidylethanolamine （PEG-DSPE） was incorporated into polymer-lipid hybrid nanoparticles （PLN） to construct a PEG-DSPE modified long circulating PLN （L-PLN）. The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry.
Results： The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes （N/P=10） was about 37.2%, which was higher than those transfected by PLN alone or commercial LipofectamineTM 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors.
Conclusion： L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy. |
| doi_str_mv | 10.1038/aps.2010.15 |
| format | Article |
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Methods： Poly （ethylene glycol）-distearoylphosphatidylethanolamine （PEG-DSPE） was incorporated into polymer-lipid hybrid nanoparticles （PLN） to construct a PEG-DSPE modified long circulating PLN （L-PLN）. The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry.
Results： The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes （N/P=10） was about 37.2%, which was higher than those transfected by PLN alone or commercial LipofectamineTM 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors.
Conclusion： L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1038/aps.2010.15</identifier><identifier>PMID: 20348944</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell Line ; Cell Line, Tumor ; Cell Survival ; DNA - administration & dosage ; HEK293细胞 ; Humans ; Immunology ; Internal Medicine ; Lipids - chemistry ; Medical Microbiology ; Nanoparticles - chemistry ; Original ; original-article ; Pharmacology/Toxicology ; Phosphatidylethanolamines - chemistry ; Polyethylene Glycols - chemistry ; Transfection ; Vaccine ; 基因传递 ; 病毒基因 ; 纳米粒子 ; 聚合物 ; 转染效率</subject><ispartof>Acta pharmacologica Sinica, 2010-04, Vol.31 (4), p.509-514</ispartof><rights>CPS and SIMM 2010</rights><rights>Copyright Nature Publishing Group Apr 2010</rights><rights>Copyright © 2010 CPS and SIMM 2010 CPS and SIMM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-9bb59d5dab92c24ab92913be64df5dadedec100f1ab872b0f7c20b0a1c69cb8f3</citedby><cites>FETCH-LOGICAL-c503t-9bb59d5dab92c24ab92913be64df5dadedec100f1ab872b0f7c20b0a1c69cb8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007669/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007669/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20348944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>He, Ying-zi</creatorcontrib><creatorcontrib>Li, Wen</creatorcontrib><creatorcontrib>Shen, Yun-zhen</creatorcontrib><creatorcontrib>Li, Yu-ru</creatorcontrib><creatorcontrib>Wang, Yun-feng</creatorcontrib><title>A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity.
Methods： Poly （ethylene glycol）-distearoylphosphatidylethanolamine （PEG-DSPE） was incorporated into polymer-lipid hybrid nanoparticles （PLN） to construct a PEG-DSPE modified long circulating PLN （L-PLN）. The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry.
Results： The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes （N/P=10） was about 37.2%, which was higher than those transfected by PLN alone or commercial LipofectamineTM 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors.
Conclusion： L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>DNA - administration & dosage</subject><subject>HEK293细胞</subject><subject>Humans</subject><subject>Immunology</subject><subject>Internal Medicine</subject><subject>Lipids - chemistry</subject><subject>Medical Microbiology</subject><subject>Nanoparticles - chemistry</subject><subject>Original</subject><subject>original-article</subject><subject>Pharmacology/Toxicology</subject><subject>Phosphatidylethanolamines - chemistry</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Transfection</subject><subject>Vaccine</subject><subject>基因传递</subject><subject>病毒基因</subject><subject>纳米粒子</subject><subject>聚合物</subject><subject>转染效率</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkUtvEzEUha0K1Edg1T0asWHRTvFzZrxBqiraIlWwgbVle64TF8ee2kmk_HscJZSCWF3b9_PxuT4InRN8RTAbPuqpXFG824kjdEp6LtqeCv6qrruetBwP7ASdlfKIMaOMyGN0QjHjg-T8FH29bmLaQGimFLZLyG3wkx-bxdbkWqKOadJ55W2AxqXcgHPeeoireitufNahmUOEZoTgN5C3b9Brp0OBt4c6Qz9uP3-_uW8fvt19ubl-aK3AbNVKY4QcxaiNpJbyXZGEGej46OrpCCNYgrEj2gw9Ndj1lmKDNbGdtGZwbIY-7XWntVnCaKuj6kVN2S913qqkvfq7E_1CzdNGcYz7rpNV4MNBIKenNZSVWvpiIQQdIa2L6jnvqOjrB8_Q-3_Ix7TOsU6nKGGYDpIOFbrYQzanUjK4ZysEq11KqqakdikpIir97qX7Z_Z3LBW43AOltuIc8p83_693sGgXKc6f6g1ltP3pfADF6sSECMp-AUspqes</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Li, Jian</creator><creator>He, Ying-zi</creator><creator>Li, Wen</creator><creator>Shen, Yun-zhen</creator><creator>Li, Yu-ru</creator><creator>Wang, Yun-feng</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>5PM</scope></search><sort><creationdate>20100401</creationdate><title>A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery</title><author>Li, Jian ; He, Ying-zi ; Li, Wen ; Shen, Yun-zhen ; Li, Yu-ru ; Wang, Yun-feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-9bb59d5dab92c24ab92913be64df5dadedec100f1ab872b0f7c20b0a1c69cb8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>DNA - administration & dosage</topic><topic>HEK293细胞</topic><topic>Humans</topic><topic>Immunology</topic><topic>Internal Medicine</topic><topic>Lipids - chemistry</topic><topic>Medical Microbiology</topic><topic>Nanoparticles - chemistry</topic><topic>Original</topic><topic>original-article</topic><topic>Pharmacology/Toxicology</topic><topic>Phosphatidylethanolamines - chemistry</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Transfection</topic><topic>Vaccine</topic><topic>基因传递</topic><topic>病毒基因</topic><topic>纳米粒子</topic><topic>聚合物</topic><topic>转染效率</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jian</creatorcontrib><creatorcontrib>He, Ying-zi</creatorcontrib><creatorcontrib>Li, Wen</creatorcontrib><creatorcontrib>Shen, Yun-zhen</creatorcontrib><creatorcontrib>Li, Yu-ru</creatorcontrib><creatorcontrib>Wang, Yun-feng</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jian</au><au>He, Ying-zi</au><au>Li, Wen</au><au>Shen, Yun-zhen</au><au>Li, Yu-ru</au><au>Wang, Yun-feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery</atitle><jtitle>Acta pharmacologica Sinica</jtitle><stitle>Acta Pharmacol Sin</stitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>31</volume><issue>4</issue><spage>509</spage><epage>514</epage><pages>509-514</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>Aim： To develop a novel non-viral vector with high transfection efficiency and low cytotoxicity.
Methods： Poly （ethylene glycol）-distearoylphosphatidylethanolamine （PEG-DSPE） was incorporated into polymer-lipid hybrid nanoparticles （PLN） to construct a PEG-DSPE modified long circulating PLN （L-PLN）. The L-PLN was prepared by the emulsifying-solvent evaporation method, L-PLN and L-PLN/DNA complexes were characterized. Both HEK293 and MDA-MB-231 cells transfected by L-PLN/DNA complexes were observed under a fluorescence microscope. The transfection efficiency of the complexes to HEK293 cells was further evaluated by flow cytometry.
Results： The GFP fluorescence intensity in HEK293 cells transfected by the L-PLN/DNA complexes （N/P=10） was about 37.2%, which was higher than those transfected by PLN alone or commercial LipofectamineTM 2000. The L-PLN exhibited minimal toxicity at a low N/P ratio compared with other vectors.
Conclusion： L-PLN as a novel gene delivery system, has higher transfection efficiency and acceptable cytotoxicity compared to the corresponding PLN, which is beneficial for the development of non-viral gene transfer vectors and may offer an alternative strategy for the future gene therapy.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20348944</pmid><doi>10.1038/aps.2010.15</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cell Line Cell Line, Tumor Cell Survival DNA - administration & dosage HEK293细胞 Humans Immunology Internal Medicine Lipids - chemistry Medical Microbiology Nanoparticles - chemistry Original original-article Pharmacology/Toxicology Phosphatidylethanolamines - chemistry Polyethylene Glycols - chemistry Transfection Vaccine 基因传递 病毒基因 纳米粒子 聚合物 转染效率 |
| title | A novel polymer-lipid hybrid nanoparticle for efficient nonviral gene delivery |
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