Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro
Aim： To optimize formulation methods for loading gemcitabine （GEM）, the main drug against pancreatic cancer, into albumin nanoparticles for extended blood circulation and improved efficacy. Methods： GEM was loaded into two sizes of disolvation-crosslinked bovine serum albumin nanoparticles, with a m...
Gespeichert in:
| Veröffentlicht in: | Acta pharmacologica Sinica 2009-09, Vol.30 (9), p.1337-1343 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1343 |
|---|---|
| container_issue | 9 |
| container_start_page | 1337 |
| container_title | Acta pharmacologica Sinica |
| container_volume | 30 |
| creator | Li, Jin-ming Chen, Wei Wang, Hao Jin, Chen Yu, Xian-jun Lu, Wei-yue Cui, Long Fu, De-liang Ni, Quan-xing Hou, Hui-min |
| description | Aim： To optimize formulation methods for loading gemcitabine （GEM）, the main drug against pancreatic cancer, into albumin nanoparticles for extended blood circulation and improved efficacy. Methods： GEM was loaded into two sizes of disolvation-crosslinked bovine serum albumin nanoparticles, with a mean diameter of 109.7 nm and 405.6 nm, respectively, by corecipitation （the direct method） and follow-up adsorption （the indirect method）. The antitumor activities of the two nanoparticulate formulations, were evaluated according to their anti-proliferative effects on the human pan- creatic cell line BXPC-3, which were assessed using the MTT assay. Results： The two nanoparticulate formulations, created by direct co-precipitation and indirect adsorption, possessed smooth surfaces and high drug loading efficiencies, 83% and 93% at 11% and 13% drug loading, respectively. The two formulations released GEM for 8 and 12 h, respectively, and significantly improved anti-BXPC-3 proliferation effects, as compared with the GEM solution and the drugfree albumin particles. Conclusion： Co-precipitating and adsorbing GEM into albumin particles resulted in sustained-release nanoparticulate formulations with improved antitumor cytotoxicity. The result suggests that this is a useful formulation strategy for improving the antitumor efficacy of GEM. |
| doi_str_mv | 10.1038/aps.2009.125 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4007180</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>31518486</cqvip_id><sourcerecordid>1857225251</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-7cfc9f4b31e39b28ceeb876805e6ad985519ab2eb664d776bd488c2a364f8fed3</originalsourceid><addsrcrecordid>eNp1kc2LFDEQxRtR3HX15lmCZ3vMZyd9EXRwVVhwDwreQpKu7s7ak8wmmdX57zfDDK4ePFXB-9WrB69pXhK8Ipipt2abVxTjfkWoeNScE8lFK6ngj-veSdJyrNhZ8yznG4wZZaR_2pyRXjLMaX_exOsEW5NM8TGgOCKz2N3GBxRMiHk7Q4KMlmgGGNAvX2Y0wcb5YqwPgEwYUJnBJ-T2JZb421dpj8xkfMgFffhxvW4ZcrAsGVXLO19SfN48Gc2S4cVpXjTfLz9-W39ur75--rJ-f9U6LkVppRtdP3LLCLDeUuUArJKdwgI6M_RKCNIbS8F2HR-k7OzAlXLUsI6PaoSBXTTvjr7bnd3A4CCUZBa9TX5j0l5H4_W_SvCznuKd5hhLonA1eH0ySPF2B7nom7hLoWbWlDBMOyEO0Jsj5FLMOcH45wHB-tCOru3oQzu6tlPxV3-HeoBPdVSgPQK5SmGC9PD0P4ankG6OYbqtJ9oa93P0C2hGBFFcdeweahSoQw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213026550</pqid></control><display><type>article</type><title>Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Li, Jin-ming ; Chen, Wei ; Wang, Hao ; Jin, Chen ; Yu, Xian-jun ; Lu, Wei-yue ; Cui, Long ; Fu, De-liang ; Ni, Quan-xing ; Hou, Hui-min</creator><creatorcontrib>Li, Jin-ming ; Chen, Wei ; Wang, Hao ; Jin, Chen ; Yu, Xian-jun ; Lu, Wei-yue ; Cui, Long ; Fu, De-liang ; Ni, Quan-xing ; Hou, Hui-min</creatorcontrib><description>Aim： To optimize formulation methods for loading gemcitabine （GEM）, the main drug against pancreatic cancer, into albumin nanoparticles for extended blood circulation and improved efficacy. Methods： GEM was loaded into two sizes of disolvation-crosslinked bovine serum albumin nanoparticles, with a mean diameter of 109.7 nm and 405.6 nm, respectively, by corecipitation （the direct method） and follow-up adsorption （the indirect method）. The antitumor activities of the two nanoparticulate formulations, were evaluated according to their anti-proliferative effects on the human pan- creatic cell line BXPC-3, which were assessed using the MTT assay. Results： The two nanoparticulate formulations, created by direct co-precipitation and indirect adsorption, possessed smooth surfaces and high drug loading efficiencies, 83% and 93% at 11% and 13% drug loading, respectively. The two formulations released GEM for 8 and 12 h, respectively, and significantly improved anti-BXPC-3 proliferation effects, as compared with the GEM solution and the drugfree albumin particles. Conclusion： Co-precipitating and adsorbing GEM into albumin particles resulted in sustained-release nanoparticulate formulations with improved antitumor cytotoxicity. The result suggests that this is a useful formulation strategy for improving the antitumor efficacy of GEM.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1038/aps.2009.125</identifier><identifier>PMID: 19730429</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Antimetabolites, Antineoplastic - administration & dosage ; Antimetabolites, Antineoplastic - therapeutic use ; Biomedical and Life Sciences ; Biomedicine ; Deoxycytidine - administration & dosage ; Deoxycytidine - analogs & derivatives ; Deoxycytidine - therapeutic use ; Drug Carriers - therapeutic use ; Humans ; Immunology ; Internal Medicine ; Medical Microbiology ; Nanocapsules - therapeutic use ; Nanospheres - therapeutic use ; Original ; original-article ; Pancreatic Neoplasms - drug therapy ; Pharmacology/Toxicology ; Serum Albumin, Bovine - metabolism ; Tumor Cells, Cultured ; Vaccine ; 抗肿瘤活性 ; 牛血清白蛋白 ; 纳米球 ; 纳米粒子 ; 细胞株 ; 胰腺癌</subject><ispartof>Acta pharmacologica Sinica, 2009-09, Vol.30 (9), p.1337-1343</ispartof><rights>CPS and SIMM 2009</rights><rights>Copyright Nature Publishing Group Sep 2009</rights><rights>Copyright © 2009 CPS and SIMM 2009 CPS and SIMM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-7cfc9f4b31e39b28ceeb876805e6ad985519ab2eb664d776bd488c2a364f8fed3</citedby><cites>FETCH-LOGICAL-c475t-7cfc9f4b31e39b28ceeb876805e6ad985519ab2eb664d776bd488c2a364f8fed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007180/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007180/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19730429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jin-ming</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><creatorcontrib>Jin, Chen</creatorcontrib><creatorcontrib>Yu, Xian-jun</creatorcontrib><creatorcontrib>Lu, Wei-yue</creatorcontrib><creatorcontrib>Cui, Long</creatorcontrib><creatorcontrib>Fu, De-liang</creatorcontrib><creatorcontrib>Ni, Quan-xing</creatorcontrib><creatorcontrib>Hou, Hui-min</creatorcontrib><title>Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： To optimize formulation methods for loading gemcitabine （GEM）, the main drug against pancreatic cancer, into albumin nanoparticles for extended blood circulation and improved efficacy. Methods： GEM was loaded into two sizes of disolvation-crosslinked bovine serum albumin nanoparticles, with a mean diameter of 109.7 nm and 405.6 nm, respectively, by corecipitation （the direct method） and follow-up adsorption （the indirect method）. The antitumor activities of the two nanoparticulate formulations, were evaluated according to their anti-proliferative effects on the human pan- creatic cell line BXPC-3, which were assessed using the MTT assay. Results： The two nanoparticulate formulations, created by direct co-precipitation and indirect adsorption, possessed smooth surfaces and high drug loading efficiencies, 83% and 93% at 11% and 13% drug loading, respectively. The two formulations released GEM for 8 and 12 h, respectively, and significantly improved anti-BXPC-3 proliferation effects, as compared with the GEM solution and the drugfree albumin particles. Conclusion： Co-precipitating and adsorbing GEM into albumin particles resulted in sustained-release nanoparticulate formulations with improved antitumor cytotoxicity. The result suggests that this is a useful formulation strategy for improving the antitumor efficacy of GEM.</description><subject>Antimetabolites, Antineoplastic - administration & dosage</subject><subject>Antimetabolites, Antineoplastic - therapeutic use</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Deoxycytidine - administration & dosage</subject><subject>Deoxycytidine - analogs & derivatives</subject><subject>Deoxycytidine - therapeutic use</subject><subject>Drug Carriers - therapeutic use</subject><subject>Humans</subject><subject>Immunology</subject><subject>Internal Medicine</subject><subject>Medical Microbiology</subject><subject>Nanocapsules - therapeutic use</subject><subject>Nanospheres - therapeutic use</subject><subject>Original</subject><subject>original-article</subject><subject>Pancreatic Neoplasms - drug therapy</subject><subject>Pharmacology/Toxicology</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>Tumor Cells, Cultured</subject><subject>Vaccine</subject><subject>抗肿瘤活性</subject><subject>牛血清白蛋白</subject><subject>纳米球</subject><subject>纳米粒子</subject><subject>细胞株</subject><subject>胰腺癌</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc2LFDEQxRtR3HX15lmCZ3vMZyd9EXRwVVhwDwreQpKu7s7ak8wmmdX57zfDDK4ePFXB-9WrB69pXhK8Ipipt2abVxTjfkWoeNScE8lFK6ngj-veSdJyrNhZ8yznG4wZZaR_2pyRXjLMaX_exOsEW5NM8TGgOCKz2N3GBxRMiHk7Q4KMlmgGGNAvX2Y0wcb5YqwPgEwYUJnBJ-T2JZb421dpj8xkfMgFffhxvW4ZcrAsGVXLO19SfN48Gc2S4cVpXjTfLz9-W39ur75--rJ-f9U6LkVppRtdP3LLCLDeUuUArJKdwgI6M_RKCNIbS8F2HR-k7OzAlXLUsI6PaoSBXTTvjr7bnd3A4CCUZBa9TX5j0l5H4_W_SvCznuKd5hhLonA1eH0ySPF2B7nom7hLoWbWlDBMOyEO0Jsj5FLMOcH45wHB-tCOru3oQzu6tlPxV3-HeoBPdVSgPQK5SmGC9PD0P4ankG6OYbqtJ9oa93P0C2hGBFFcdeweahSoQw</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Li, Jin-ming</creator><creator>Chen, Wei</creator><creator>Wang, Hao</creator><creator>Jin, Chen</creator><creator>Yu, Xian-jun</creator><creator>Lu, Wei-yue</creator><creator>Cui, Long</creator><creator>Fu, De-liang</creator><creator>Ni, Quan-xing</creator><creator>Hou, Hui-min</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20090901</creationdate><title>Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro</title><author>Li, Jin-ming ; Chen, Wei ; Wang, Hao ; Jin, Chen ; Yu, Xian-jun ; Lu, Wei-yue ; Cui, Long ; Fu, De-liang ; Ni, Quan-xing ; Hou, Hui-min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-7cfc9f4b31e39b28ceeb876805e6ad985519ab2eb664d776bd488c2a364f8fed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antimetabolites, Antineoplastic - administration & dosage</topic><topic>Antimetabolites, Antineoplastic - therapeutic use</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Deoxycytidine - administration & dosage</topic><topic>Deoxycytidine - analogs & derivatives</topic><topic>Deoxycytidine - therapeutic use</topic><topic>Drug Carriers - therapeutic use</topic><topic>Humans</topic><topic>Immunology</topic><topic>Internal Medicine</topic><topic>Medical Microbiology</topic><topic>Nanocapsules - therapeutic use</topic><topic>Nanospheres - therapeutic use</topic><topic>Original</topic><topic>original-article</topic><topic>Pancreatic Neoplasms - drug therapy</topic><topic>Pharmacology/Toxicology</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>Tumor Cells, Cultured</topic><topic>Vaccine</topic><topic>抗肿瘤活性</topic><topic>牛血清白蛋白</topic><topic>纳米球</topic><topic>纳米粒子</topic><topic>细胞株</topic><topic>胰腺癌</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jin-ming</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><creatorcontrib>Jin, Chen</creatorcontrib><creatorcontrib>Yu, Xian-jun</creatorcontrib><creatorcontrib>Lu, Wei-yue</creatorcontrib><creatorcontrib>Cui, Long</creatorcontrib><creatorcontrib>Fu, De-liang</creatorcontrib><creatorcontrib>Ni, Quan-xing</creatorcontrib><creatorcontrib>Hou, Hui-min</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jin-ming</au><au>Chen, Wei</au><au>Wang, Hao</au><au>Jin, Chen</au><au>Yu, Xian-jun</au><au>Lu, Wei-yue</au><au>Cui, Long</au><au>Fu, De-liang</au><au>Ni, Quan-xing</au><au>Hou, Hui-min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro</atitle><jtitle>Acta pharmacologica Sinica</jtitle><stitle>Acta Pharmacol Sin</stitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>30</volume><issue>9</issue><spage>1337</spage><epage>1343</epage><pages>1337-1343</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>Aim： To optimize formulation methods for loading gemcitabine （GEM）, the main drug against pancreatic cancer, into albumin nanoparticles for extended blood circulation and improved efficacy. Methods： GEM was loaded into two sizes of disolvation-crosslinked bovine serum albumin nanoparticles, with a mean diameter of 109.7 nm and 405.6 nm, respectively, by corecipitation （the direct method） and follow-up adsorption （the indirect method）. The antitumor activities of the two nanoparticulate formulations, were evaluated according to their anti-proliferative effects on the human pan- creatic cell line BXPC-3, which were assessed using the MTT assay. Results： The two nanoparticulate formulations, created by direct co-precipitation and indirect adsorption, possessed smooth surfaces and high drug loading efficiencies, 83% and 93% at 11% and 13% drug loading, respectively. The two formulations released GEM for 8 and 12 h, respectively, and significantly improved anti-BXPC-3 proliferation effects, as compared with the GEM solution and the drugfree albumin particles. Conclusion： Co-precipitating and adsorbing GEM into albumin particles resulted in sustained-release nanoparticulate formulations with improved antitumor cytotoxicity. The result suggests that this is a useful formulation strategy for improving the antitumor efficacy of GEM.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>19730429</pmid><doi>10.1038/aps.2009.125</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1671-4083 |
| ispartof | Acta pharmacologica Sinica, 2009-09, Vol.30 (9), p.1337-1343 |
| issn | 1671-4083 1745-7254 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4007180 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antimetabolites, Antineoplastic - administration & dosage Antimetabolites, Antineoplastic - therapeutic use Biomedical and Life Sciences Biomedicine Deoxycytidine - administration & dosage Deoxycytidine - analogs & derivatives Deoxycytidine - therapeutic use Drug Carriers - therapeutic use Humans Immunology Internal Medicine Medical Microbiology Nanocapsules - therapeutic use Nanospheres - therapeutic use Original original-article Pancreatic Neoplasms - drug therapy Pharmacology/Toxicology Serum Albumin, Bovine - metabolism Tumor Cells, Cultured Vaccine 抗肿瘤活性 牛血清白蛋白 纳米球 纳米粒子 细胞株 胰腺癌 |
| title | Preparation of albumin nanospheres loaded with gemcitabine and their cytotoxicity against BXPC-3 cells in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T15%3A31%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20of%20albumin%20nanospheres%20loaded%20with%20gemcitabine%20and%20their%20cytotoxicity%20against%20BXPC-3%20cells%20in%20vitro&rft.jtitle=Acta%20pharmacologica%20Sinica&rft.au=Li,%20Jin-ming&rft.date=2009-09-01&rft.volume=30&rft.issue=9&rft.spage=1337&rft.epage=1343&rft.pages=1337-1343&rft.issn=1671-4083&rft.eissn=1745-7254&rft_id=info:doi/10.1038/aps.2009.125&rft_dat=%3Cproquest_pubme%3E1857225251%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=213026550&rft_id=info:pmid/19730429&rft_cqvip_id=31518486&rfr_iscdi=true |