Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications
Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with...
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| Veröffentlicht in: | ACS nano 2014-04, Vol.8 (4), p.3913-3920 |
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| creator | Chiu, Ya-Ling Chan, Hon Fai Phua, Kyle K. L Zhang, Ying Juul, Sissel Knudsen, Birgitta R Ho, Yi-Ping Leong, Kam W |
| description | Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions. |
| doi_str_mv | 10.1021/nn500810n |
| format | Article |
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Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.</description><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemical synthesis</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biological</subject><subject>Chemistry Techniques, Synthetic</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>DNA Topoisomerases, Type I - metabolism</subject><subject>Droplets</subject><subject>Emulsions</subject><subject>Enzyme Assays</subject><subject>Ethers - chemical synthesis</subject><subject>Ethers - chemistry</subject><subject>Ethers - pharmacology</subject><subject>Fluorocarbons - chemical synthesis</subject><subject>Fluorocarbons - chemistry</subject><subject>Fluorocarbons - pharmacology</subject><subject>Halogenation</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Nanostructure</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surface-Active Agents - chemical synthesis</subject><subject>Surface-Active Agents - chemistry</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Surfactants</subject><subject>Synthesis</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>N~.</sourceid><sourceid>EIF</sourceid><recordid>eNqNkU9LxDAQxYMo_lk9-AWkF0EP1UmapO1FcMVVQfCggreQTSdrJZusSSv47a2sLgoePM2D-fGYeY-QfQonFBg99V4AVBT8GtmmdSFzqOTT-koLukV2UnoBEGVVyk2yxbjkEqpqm9zcv_vuGVObsmCzietDDKmPVptO-y5lNsTsct671Aafj3XCJhu3wYVZa7TLzhcLN4huWKZdsmG1S7j3NUfkcXL5cHGd395d3Vyc3-ZaAO9yQ4FzY1iFlvOCghQWWdEANMgtNqALUSOtrNQWpK2nJTKpeTNFXdMShShG5Gzpu-inc2wM-i5qpxaxnev4roJu1e-Nb5_VLLwpDsALxgaDoy-DGF57TJ2at8mgc9pj6JOipWQgeA3_QAWtJK05gwE9XqJmCDBFtKuLKKjPltSqpYE9-PnCivyuZQAOl4A2Sb2EPvoh0T-MPgCvtpqz</recordid><startdate>20140422</startdate><enddate>20140422</enddate><creator>Chiu, Ya-Ling</creator><creator>Chan, Hon Fai</creator><creator>Phua, Kyle K. L</creator><creator>Zhang, Ying</creator><creator>Juul, Sissel</creator><creator>Knudsen, Birgitta R</creator><creator>Ho, Yi-Ping</creator><creator>Leong, Kam W</creator><general>American Chemical Society</general><scope>N~.</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>5PM</scope></search><sort><creationdate>20140422</creationdate><title>Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications</title><author>Chiu, Ya-Ling ; Chan, Hon Fai ; Phua, Kyle K. 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L</au><au>Zhang, Ying</au><au>Juul, Sissel</au><au>Knudsen, Birgitta R</au><au>Ho, Yi-Ping</au><au>Leong, Kam W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2014-04-22</date><risdate>2014</risdate><volume>8</volume><issue>4</issue><spage>3913</spage><epage>3920</epage><pages>3913-3920</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24646088</pmid><doi>10.1021/nn500810n</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ACS Publications |
| subjects | Bacteria Biocompatibility Biocompatible Materials - chemical synthesis Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biological Chemistry Techniques, Synthetic DNA - chemistry DNA - metabolism DNA Topoisomerases, Type I - metabolism Droplets Emulsions Enzyme Assays Ethers - chemical synthesis Ethers - chemistry Ethers - pharmacology Fluorocarbons - chemical synthesis Fluorocarbons - chemistry Fluorocarbons - pharmacology Halogenation Humans Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Nanostructure Spectroscopy, Fourier Transform Infrared Surface-Active Agents - chemical synthesis Surface-Active Agents - chemistry Surface-Active Agents - pharmacology Surfactants Synthesis |
| title | Synthesis of Fluorosurfactants for Emulsion-Based Biological Applications |
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