Globally Visualizing the Microtubule-Dependent Transport Behaviors of Influenza Virus in Live Cells
Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing the mechanisms of virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent trans...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2014-04, Vol.86 (8), p.3902-3908 |
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| creator | Liu, Shu-Lin Zhang, Li-Juan Wang, Zhi-Gang Zhang, Zhi-Ling Wu, Qiu-Mei Sun, En-Ze Shi, Yun-Bo Pang, Dai-Wen |
| description | Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing the mechanisms of virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent transport behaviors of influenza virus in live cells. We found that the intersection configuration of microtubules can interfere with the transport behaviors of the virus in live cells, which lead to the changing and long-time pausing of the transport behavior of viruses. Our results revealed that most of the viruses moved along straight microtubules rapidly and unidirectionally from the cell periphery to the microtubule organizing center (MTOC) near the bottom of the cell, and the viruses were confined in the grid of microtubules near the top of the cell and at the MTOC near the bottom of the cell. These results provided deep insights into the influence of entire microtubule geometry on the virus infection. |
| doi_str_mv | 10.1021/ac500640u |
| format | Article |
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Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent transport behaviors of influenza virus in live cells. We found that the intersection configuration of microtubules can interfere with the transport behaviors of the virus in live cells, which lead to the changing and long-time pausing of the transport behavior of viruses. Our results revealed that most of the viruses moved along straight microtubules rapidly and unidirectionally from the cell periphery to the microtubule organizing center (MTOC) near the bottom of the cell, and the viruses were confined in the grid of microtubules near the top of the cell and at the MTOC near the bottom of the cell. These results provided deep insights into the influence of entire microtubule geometry on the virus infection.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac500640u</identifier><identifier>PMID: 24678700</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Animals ; Cells ; Cells - ultrastructure ; Cells - virology ; Dogs ; Endocytosis ; geometry ; Humans ; Image Processing, Computer-Assisted ; Influenza ; Influenza A virus ; Influenza virus ; Influenza, Human - virology ; Intersections ; Madin Darby Canine Kidney Cells ; Microscopy, Fluorescence ; microtubules ; Microtubules - ultrastructure ; Microtubules - virology ; Organizing ; Orthomyxoviridae - ultrastructure ; Quantum Dots ; Tracking ; Transport ; Viral Envelope Proteins - chemistry ; Viruses</subject><ispartof>Analytical chemistry (Washington), 2014-04, Vol.86 (8), p.3902-3908</ispartof><rights>Copyright © 2014 American Chemical Society</rights><rights>Copyright American Chemical Society Apr 15, 2014</rights><rights>Copyright © 2014 American Chemical Society 2014 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a598t-786f9cf79a7b9290c761ee81a7fd72289056d12d9d334aa2fa82daa3ebb5126b3</citedby><cites>FETCH-LOGICAL-a598t-786f9cf79a7b9290c761ee81a7fd72289056d12d9d334aa2fa82daa3ebb5126b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac500640u$$EPDF$$P50$$Gacs$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac500640u$$EHTML$$P50$$Gacs$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24678700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Shu-Lin</creatorcontrib><creatorcontrib>Zhang, Li-Juan</creatorcontrib><creatorcontrib>Wang, Zhi-Gang</creatorcontrib><creatorcontrib>Zhang, Zhi-Ling</creatorcontrib><creatorcontrib>Wu, Qiu-Mei</creatorcontrib><creatorcontrib>Sun, En-Ze</creatorcontrib><creatorcontrib>Shi, Yun-Bo</creatorcontrib><creatorcontrib>Pang, Dai-Wen</creatorcontrib><title>Globally Visualizing the Microtubule-Dependent Transport Behaviors of Influenza Virus in Live Cells</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing the mechanisms of virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent transport behaviors of influenza virus in live cells. We found that the intersection configuration of microtubules can interfere with the transport behaviors of the virus in live cells, which lead to the changing and long-time pausing of the transport behavior of viruses. Our results revealed that most of the viruses moved along straight microtubules rapidly and unidirectionally from the cell periphery to the microtubule organizing center (MTOC) near the bottom of the cell, and the viruses were confined in the grid of microtubules near the top of the cell and at the MTOC near the bottom of the cell. These results provided deep insights into the influence of entire microtubule geometry on the virus infection.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Cells</subject><subject>Cells - ultrastructure</subject><subject>Cells - virology</subject><subject>Dogs</subject><subject>Endocytosis</subject><subject>geometry</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Influenza</subject><subject>Influenza A virus</subject><subject>Influenza virus</subject><subject>Influenza, Human - virology</subject><subject>Intersections</subject><subject>Madin Darby Canine Kidney Cells</subject><subject>Microscopy, Fluorescence</subject><subject>microtubules</subject><subject>Microtubules - ultrastructure</subject><subject>Microtubules - virology</subject><subject>Organizing</subject><subject>Orthomyxoviridae - ultrastructure</subject><subject>Quantum Dots</subject><subject>Tracking</subject><subject>Transport</subject><subject>Viral Envelope Proteins - chemistry</subject><subject>Viruses</subject><issn>0003-2700</issn><issn>1520-6882</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>N~.</sourceid><sourceid>EIF</sourceid><recordid>eNqFkkFrFDEUx4Modq0e_AISEEEPoy-ZzCS5CLqttbDipXod3sxkuinZZE0mC-2nN2XrUvXQU-Dlxy95__cIecngPQPOPuDQALQC8iOyYA2HqlWKPyYLAKgrLgGOyLOUrgAYA9Y-JUdctFKV-oIMZy706Nw1_WlTRmdvrL-k89rQb3aIYc59dqY6MVvjR-NnehHRp22IM_1s1rizISYaJnruJ5eNv8GiiTlR6-nK7gxdGufSc_JkQpfMi7vzmPz4cnqx_Fqtvp-dLz-tKmy0miup2kkPk9Qoe801DLJlxiiGchol50pD046Mj3qsa4HIJ1R8RKxN3zeMt319TD7uvdvcb8w4lP9GdN022g3G6y6g7f6-8XbdXYZdJwAE07wI3t4JYviVTZq7jU1DaQG9CTl1vATKRaOZeBBlbfFBLZh8GG1qococxS36-h_0KuToS2iFYlpwJhtWqHd7qgwopWimQ4sMutuF6A4LUdhX9zM5kH82oABv9gAO6d5r_4l-A-DsvHs</recordid><startdate>20140415</startdate><enddate>20140415</enddate><creator>Liu, Shu-Lin</creator><creator>Zhang, Li-Juan</creator><creator>Wang, Zhi-Gang</creator><creator>Zhang, Zhi-Ling</creator><creator>Wu, Qiu-Mei</creator><creator>Sun, En-Ze</creator><creator>Shi, Yun-Bo</creator><creator>Pang, Dai-Wen</creator><general>American Chemical Society</general><scope>N~.</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7T2</scope><scope>7U2</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20140415</creationdate><title>Globally Visualizing the Microtubule-Dependent Transport Behaviors of Influenza Virus in Live Cells</title><author>Liu, Shu-Lin ; Zhang, Li-Juan ; Wang, Zhi-Gang ; Zhang, Zhi-Ling ; Wu, Qiu-Mei ; Sun, En-Ze ; Shi, Yun-Bo ; Pang, Dai-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a598t-786f9cf79a7b9290c761ee81a7fd72289056d12d9d334aa2fa82daa3ebb5126b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Cells</topic><topic>Cells - ultrastructure</topic><topic>Cells - virology</topic><topic>Dogs</topic><topic>Endocytosis</topic><topic>geometry</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Influenza</topic><topic>Influenza A virus</topic><topic>Influenza virus</topic><topic>Influenza, Human - virology</topic><topic>Intersections</topic><topic>Madin Darby Canine Kidney Cells</topic><topic>Microscopy, Fluorescence</topic><topic>microtubules</topic><topic>Microtubules - ultrastructure</topic><topic>Microtubules - virology</topic><topic>Organizing</topic><topic>Orthomyxoviridae - ultrastructure</topic><topic>Quantum Dots</topic><topic>Tracking</topic><topic>Transport</topic><topic>Viral Envelope Proteins - chemistry</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Shu-Lin</creatorcontrib><creatorcontrib>Zhang, Li-Juan</creatorcontrib><creatorcontrib>Wang, Zhi-Gang</creatorcontrib><creatorcontrib>Zhang, Zhi-Ling</creatorcontrib><creatorcontrib>Wu, Qiu-Mei</creatorcontrib><creatorcontrib>Sun, En-Ze</creatorcontrib><creatorcontrib>Shi, Yun-Bo</creatorcontrib><creatorcontrib>Pang, Dai-Wen</creatorcontrib><collection>American Chemical Society (ACS) Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Shu-Lin</au><au>Zhang, Li-Juan</au><au>Wang, Zhi-Gang</au><au>Zhang, Zhi-Ling</au><au>Wu, Qiu-Mei</au><au>Sun, En-Ze</au><au>Shi, Yun-Bo</au><au>Pang, Dai-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Globally Visualizing the Microtubule-Dependent Transport Behaviors of Influenza Virus in Live Cells</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2014-04-15</date><risdate>2014</risdate><volume>86</volume><issue>8</issue><spage>3902</spage><epage>3908</epage><pages>3902-3908</pages><issn>0003-2700</issn><issn>1520-6882</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing the mechanisms of virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically and globally visualize the microtubule-dependent transport behaviors of influenza virus in live cells. We found that the intersection configuration of microtubules can interfere with the transport behaviors of the virus in live cells, which lead to the changing and long-time pausing of the transport behavior of viruses. Our results revealed that most of the viruses moved along straight microtubules rapidly and unidirectionally from the cell periphery to the microtubule organizing center (MTOC) near the bottom of the cell, and the viruses were confined in the grid of microtubules near the top of the cell and at the MTOC near the bottom of the cell. These results provided deep insights into the influence of entire microtubule geometry on the virus infection.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24678700</pmid><doi>10.1021/ac500640u</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry Animals Cells Cells - ultrastructure Cells - virology Dogs Endocytosis geometry Humans Image Processing, Computer-Assisted Influenza Influenza A virus Influenza virus Influenza, Human - virology Intersections Madin Darby Canine Kidney Cells Microscopy, Fluorescence microtubules Microtubules - ultrastructure Microtubules - virology Organizing Orthomyxoviridae - ultrastructure Quantum Dots Tracking Transport Viral Envelope Proteins - chemistry Viruses |
| title | Globally Visualizing the Microtubule-Dependent Transport Behaviors of Influenza Virus in Live Cells |
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