Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells
To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism. Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the c...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2014-01, Vol.2014 (2014), p.1-9 |
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| creator | Sun, Dalin Cui, Yu-Gui Zhang, Xindong Jin, Baofang Wang, Zhiqiang Gao, Chao |
| description | To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism. Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells. Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf. Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism. Results. After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly. The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells. Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract. Conclusion. YC extract could stimulate the proliferation of GC-1 spg cells. Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells. |
| doi_str_mv | 10.1155/2014/640857 |
| format | Article |
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Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells. Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf. Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism. Results. After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly. The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells. Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract. Conclusion. YC extract could stimulate the proliferation of GC-1 spg cells. Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2014/640857</identifier><identifier>PMID: 24817900</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>1-Phosphatidylinositol 3-kinase ; Apoptosis ; Cell cycle ; Cell growth ; Cell proliferation ; Cell self-renewal ; Flow cytometry ; Gene expression ; Infertility ; Kinases ; mRNA ; Oct-4 protein ; Proteins ; Rodents ; siRNA ; Sperm ; Spermatogenesis ; Spermatogonia ; Stem cells</subject><ispartof>Evidence-based complementary and alternative medicine, 2014-01, Vol.2014 (2014), p.1-9</ispartof><rights>Copyright © 2014 Zhiqiang Wang et al.</rights><rights>Copyright © 2014 Zhiqiang Wang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2014 Zhiqiang Wang et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-cb308023fb9babe0629161aada7aa02d13fb633c305217e0660e124ea4175b113</citedby><cites>FETCH-LOGICAL-c566t-cb308023fb9babe0629161aada7aa02d13fb633c305217e0660e124ea4175b113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003789/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003789/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24817900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tang, Yuping</contributor><contributor>Yuping Tang</contributor><creatorcontrib>Sun, Dalin</creatorcontrib><creatorcontrib>Cui, Yu-Gui</creatorcontrib><creatorcontrib>Zhang, Xindong</creatorcontrib><creatorcontrib>Jin, Baofang</creatorcontrib><creatorcontrib>Wang, Zhiqiang</creatorcontrib><creatorcontrib>Gao, Chao</creatorcontrib><title>Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism. Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells. Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf. Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism. Results. After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly. The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells. Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract. Conclusion. YC extract could stimulate the proliferation of GC-1 spg cells. Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Apoptosis</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cell self-renewal</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Infertility</subject><subject>Kinases</subject><subject>mRNA</subject><subject>Oct-4 protein</subject><subject>Proteins</subject><subject>Rodents</subject><subject>siRNA</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>Spermatogonia</subject><subject>Stem cells</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkc2LFDEQxYMo7oeevEuDF1lptyrffRGWYV2FBQUV9BSqe9KzGXo6Y9Ltx39vhlmH1YueUlC_PN6rx9gThJeISp1zQHmuJVhl7rFjNBJrya29f5jN5yN2kvMagDfGmIfsiEuLpgE4ZhdfaFytw7iqFrTN8-Cryx9Tom6q3qe4iZPPu2EIvU80hThWsa-uFjVWH7blix-G_Ig96GnI_vHte8o-vb78uHhTX7-7eru4uK47pfVUd60AC1z0bdNS60HzBjUSLckQAV9i2WghOgGKoyl7DR659CTRqBZRnLJXe93t3G78svNj8Tm4bQobSj9dpOD-3Izhxq3iNycBhLFNEXh-K5Di19nnyW1C7koEGn2cs0OjrdKmQflvVHGJSgqlCvrsL3Qd5zSWSzheQlpQWu3Mv9hTXYo5J98ffCO4XYtu16Lbt1jop3ejHtjftRXgbA_chHFJ38P_qfmC-J7uwGitaMQvKRWrzw</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Sun, Dalin</creator><creator>Cui, Yu-Gui</creator><creator>Zhang, Xindong</creator><creator>Jin, Baofang</creator><creator>Wang, Zhiqiang</creator><creator>Gao, Chao</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells</title><author>Sun, Dalin ; Cui, Yu-Gui ; Zhang, Xindong ; Jin, Baofang ; Wang, Zhiqiang ; Gao, Chao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-cb308023fb9babe0629161aada7aa02d13fb633c305217e0660e124ea4175b113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Apoptosis</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cell self-renewal</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Infertility</topic><topic>Kinases</topic><topic>mRNA</topic><topic>Oct-4 protein</topic><topic>Proteins</topic><topic>Rodents</topic><topic>siRNA</topic><topic>Sperm</topic><topic>Spermatogenesis</topic><topic>Spermatogonia</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Dalin</creatorcontrib><creatorcontrib>Cui, Yu-Gui</creatorcontrib><creatorcontrib>Zhang, Xindong</creatorcontrib><creatorcontrib>Jin, Baofang</creatorcontrib><creatorcontrib>Wang, Zhiqiang</creatorcontrib><creatorcontrib>Gao, Chao</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Dalin</au><au>Cui, Yu-Gui</au><au>Zhang, Xindong</au><au>Jin, Baofang</au><au>Wang, Zhiqiang</au><au>Gao, Chao</au><au>Tang, Yuping</au><au>Yuping Tang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism. Methods. GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract. MTT assay was performed to detect the cell viability. Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells. Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf. Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism. Results. After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly. The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells. Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract. Conclusion. YC extract could stimulate the proliferation of GC-1 spg cells. Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>24817900</pmid><doi>10.1155/2014/640857</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase Apoptosis Cell cycle Cell growth Cell proliferation Cell self-renewal Flow cytometry Gene expression Infertility Kinases mRNA Oct-4 protein Proteins Rodents siRNA Sperm Spermatogenesis Spermatogonia Stem cells |
| title | Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells |
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