Low copy target detection by Droplet Digital PCR through application of a novel open access bioinformatic pipeline, ‘definetherain’

•The requirement for protocol adaptation from qPCR to ddPCR is characterised.•A potential loss of sensitivity for ddPCR at low target numbers is reported.•A new bioinformatic tool ‘definetherain’ to improve droplet calling at low input target numbers is devised and applied.•‘Definetherain’ is made a...

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Veröffentlicht in:	Journal of virological methods 2014-06, Vol.202 (100), p.46-53
Hauptverfasser:	Jones, Mathew, Williams, James, Gärtner, Kathleen, Phillips, Rodney, Hurst, Jacob, Frater, John
Format:	Artikel
Sprache:	eng
Schlagworte:	Computational Biology - methods Droplet Digital PCR HIV-1 HIV-1 - genetics Humans k means clustering Molecular Diagnostic Techniques - methods Polymerase Chain Reaction - methods Quantitative PCR Sensitivity and Specificity Serum Albumin - genetics
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