Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo
Aim： To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. Methods： Peritoneal free cancer cells （PFCCs） were isolated from ascites specimens of gastric cancer patients, c...
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| Veröffentlicht in: | Acta pharmacologica Sinica 2012-04, Vol.33 (4), p.513-522 |
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| creator | Wang, Xiao-lei Chen, Xi-mei Fang, Jian-ping Yang, Chang-qin |
| description | Aim： To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. Methods： Peritoneal free cancer cells （PFCCs） were isolated from ascites specimens of gastric cancer patients, c-Met expression in PFCCs was detected with immunocytochemistry. In human gastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTr assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells （HPMCs） monolayer in vitro and in mice in vivo. Results： PFCCs were isolated from ascites of 6 out of 10 gastric cancer patients, c-Met expression in PFCCs was detected in 5 of the 6 gastric cancer patients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of a3131 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor progression of SGC7901 cells. Conclusion： c-Met is expressed in PFCCs from the ascites of gastric cancer patients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer. |
| doi_str_mv | 10.1038/aps.2011.205 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4003368</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>41423563</cqvip_id><sourcerecordid>1014100034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c507t-80ea94c1325e64bd6e264c7d3192ed5cb90246bbc37eeff4d3fe8b7bf3ce777b3</originalsourceid><addsrcrecordid>eNp9kstv1DAQxiMEomXhxhkZLnAgxa_YyaVSVfGSFpAQnC3HmaQuWTu1k5V64W9nVrssDwkufs1vPvvzTFE8ZvSMUVG_slM-45QxHKo7xSnTsio1r-RdXCvNSklrcVI8yPmaUsEFa-4XJ5xLqrmgp8X3NYTZb31acrmBztsZOvL54wXJfoTgfBhI7IkrP8BMNjZ9g268JXmZpgQ5QyYTJD_HAHYknceTjQ929jHssgab5-QdcTY4SMQHsvVzisSGbr_ZxofFvd6OGR4d5lXx9c3rL5fvyvWnt-8vL9alq6iey5qCbaRjglegZNsp4Eo63aEbDl3l2oZyqdrWCQ3Q97ITPdStbnvhQGvdilVxvtedlhZtOjSd7Gim5NHUrYnWmz8jwV-ZIW6NxE8TqkaB5weBFG8WyLPZ-OxgHG2AuGTTNLzG10iF5Iv_kowyySjKSkSf_YVexyUF_AjTKKl4VWORVsXLPeRSzDlBf3w1o2bXAQY7wOw6AIcK8Se_Oz3CP0uOQLkHMobCAOnXpf8QfHq4_yqG4QZTjpqSSS4qJcQP6ZnJRA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>964625872</pqid></control><display><type>article</type><title>Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Xiao-lei ; Chen, Xi-mei ; Fang, Jian-ping ; Yang, Chang-qin</creator><creatorcontrib>Wang, Xiao-lei ; Chen, Xi-mei ; Fang, Jian-ping ; Yang, Chang-qin</creatorcontrib><description>Aim： To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. Methods： Peritoneal free cancer cells （PFCCs） were isolated from ascites specimens of gastric cancer patients, c-Met expression in PFCCs was detected with immunocytochemistry. In human gastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTr assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells （HPMCs） monolayer in vitro and in mice in vivo. Results： PFCCs were isolated from ascites of 6 out of 10 gastric cancer patients, c-Met expression in PFCCs was detected in 5 of the 6 gastric cancer patients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of a3131 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor progression of SGC7901 cells. Conclusion： c-Met is expressed in PFCCs from the ascites of gastric cancer patients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1038/aps.2011.205</identifier><identifier>PMID: 22407230</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animal models ; Animals ; Ascites ; Biomedical and Life Sciences ; Biomedicine ; Cadherins - genetics ; Cell Adhesion ; Cell Line, Tumor ; Cell Proliferation ; Down-Regulation ; E-Cadherin ; Female ; Gastric cancer ; Gene expression ; Humans ; Immunocytochemistry ; Immunology ; Integrins ; Internal Medicine ; Lentivirus - genetics ; Medical Microbiology ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; mRNA ; Neoplasm Invasiveness - genetics ; Neoplasm Invasiveness - prevention & control ; Original ; original-article ; Peritoneum ; Peritoneum - cytology ; Peritoneum - pathology ; Pharmacology/Toxicology ; Polymerase chain reaction ; Proto-Oncogene Proteins c-met - genetics ; RNA Interference ; RNA-mediated interference ; RNA沉默 ; Stomach - metabolism ; Stomach - pathology ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Tumor cell lines ; Tumor Cells, Cultured ; Tumors ; Vaccine ; Western blotting ; 介导 ; 体内 ; 体外 ; 化学检测 ; 慢病毒 ; 胃癌细胞 ; 腹膜</subject><ispartof>Acta pharmacologica Sinica, 2012-04, Vol.33 (4), p.513-522</ispartof><rights>CPS and SIMM 2012</rights><rights>Copyright Nature Publishing Group Apr 2012</rights><rights>Copyright © 2012 CPS and SIMM 2012 CPS and SIMM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-80ea94c1325e64bd6e264c7d3192ed5cb90246bbc37eeff4d3fe8b7bf3ce777b3</citedby><cites>FETCH-LOGICAL-c507t-80ea94c1325e64bd6e264c7d3192ed5cb90246bbc37eeff4d3fe8b7bf3ce777b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003368/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003368/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22407230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiao-lei</creatorcontrib><creatorcontrib>Chen, Xi-mei</creatorcontrib><creatorcontrib>Fang, Jian-ping</creatorcontrib><creatorcontrib>Yang, Chang-qin</creatorcontrib><title>Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. Methods： Peritoneal free cancer cells （PFCCs） were isolated from ascites specimens of gastric cancer patients, c-Met expression in PFCCs was detected with immunocytochemistry. In human gastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTr assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells （HPMCs） monolayer in vitro and in mice in vivo. Results： PFCCs were isolated from ascites of 6 out of 10 gastric cancer patients, c-Met expression in PFCCs was detected in 5 of the 6 gastric cancer patients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of a3131 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor progression of SGC7901 cells. Conclusion： c-Met is expressed in PFCCs from the ascites of gastric cancer patients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer.</description><subject>Animal models</subject><subject>Animals</subject><subject>Ascites</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cadherins - genetics</subject><subject>Cell Adhesion</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Down-Regulation</subject><subject>E-Cadherin</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Immunology</subject><subject>Integrins</subject><subject>Internal Medicine</subject><subject>Lentivirus - genetics</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>mRNA</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Invasiveness - prevention & control</subject><subject>Original</subject><subject>original-article</subject><subject>Peritoneum</subject><subject>Peritoneum - cytology</subject><subject>Peritoneum - pathology</subject><subject>Pharmacology/Toxicology</subject><subject>Polymerase chain reaction</subject><subject>Proto-Oncogene Proteins c-met - genetics</subject><subject>RNA Interference</subject><subject>RNA-mediated interference</subject><subject>RNA沉默</subject><subject>Stomach - metabolism</subject><subject>Stomach - pathology</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tumor cell lines</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Vaccine</subject><subject>Western blotting</subject><subject>介导</subject><subject>体内</subject><subject>体外</subject><subject>化学检测</subject><subject>慢病毒</subject><subject>胃癌细胞</subject><subject>腹膜</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kstv1DAQxiMEomXhxhkZLnAgxa_YyaVSVfGSFpAQnC3HmaQuWTu1k5V64W9nVrssDwkufs1vPvvzTFE8ZvSMUVG_slM-45QxHKo7xSnTsio1r-RdXCvNSklrcVI8yPmaUsEFa-4XJ5xLqrmgp8X3NYTZb31acrmBztsZOvL54wXJfoTgfBhI7IkrP8BMNjZ9g268JXmZpgQ5QyYTJD_HAHYknceTjQ929jHssgab5-QdcTY4SMQHsvVzisSGbr_ZxofFvd6OGR4d5lXx9c3rL5fvyvWnt-8vL9alq6iey5qCbaRjglegZNsp4Eo63aEbDl3l2oZyqdrWCQ3Q97ITPdStbnvhQGvdilVxvtedlhZtOjSd7Gim5NHUrYnWmz8jwV-ZIW6NxE8TqkaB5weBFG8WyLPZ-OxgHG2AuGTTNLzG10iF5Iv_kowyySjKSkSf_YVexyUF_AjTKKl4VWORVsXLPeRSzDlBf3w1o2bXAQY7wOw6AIcK8Se_Oz3CP0uOQLkHMobCAOnXpf8QfHq4_yqG4QZTjpqSSS4qJcQP6ZnJRA</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Wang, Xiao-lei</creator><creator>Chen, Xi-mei</creator><creator>Fang, Jian-ping</creator><creator>Yang, Chang-qin</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120401</creationdate><title>Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo</title><author>Wang, Xiao-lei ; Chen, Xi-mei ; Fang, Jian-ping ; Yang, Chang-qin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-80ea94c1325e64bd6e264c7d3192ed5cb90246bbc37eeff4d3fe8b7bf3ce777b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Ascites</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cadherins - genetics</topic><topic>Cell Adhesion</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Down-Regulation</topic><topic>E-Cadherin</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>Immunology</topic><topic>Integrins</topic><topic>Internal Medicine</topic><topic>Lentivirus - genetics</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>mRNA</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Invasiveness - prevention & control</topic><topic>Original</topic><topic>original-article</topic><topic>Peritoneum</topic><topic>Peritoneum - cytology</topic><topic>Peritoneum - pathology</topic><topic>Pharmacology/Toxicology</topic><topic>Polymerase chain reaction</topic><topic>Proto-Oncogene Proteins c-met - genetics</topic><topic>RNA Interference</topic><topic>RNA-mediated interference</topic><topic>RNA沉默</topic><topic>Stomach - metabolism</topic><topic>Stomach - pathology</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tumor cell lines</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><topic>Vaccine</topic><topic>Western blotting</topic><topic>介导</topic><topic>体内</topic><topic>体外</topic><topic>化学检测</topic><topic>慢病毒</topic><topic>胃癌细胞</topic><topic>腹膜</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiao-lei</creatorcontrib><creatorcontrib>Chen, Xi-mei</creatorcontrib><creatorcontrib>Fang, Jian-ping</creatorcontrib><creatorcontrib>Yang, Chang-qin</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiao-lei</au><au>Chen, Xi-mei</au><au>Fang, Jian-ping</au><au>Yang, Chang-qin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo</atitle><jtitle>Acta pharmacologica Sinica</jtitle><stitle>Acta Pharmacol Sin</stitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>33</volume><issue>4</issue><spage>513</spage><epage>522</epage><pages>513-522</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>Aim： To investigate the expression of c-Met in peritoneal free cancer cells isolated from human gastric cancer ascites, and its relationship to peritoneal dissemination of gastric cancer. Methods： Peritoneal free cancer cells （PFCCs） were isolated from ascites specimens of gastric cancer patients, c-Met expression in PFCCs was detected with immunocytochemistry. In human gastric cancer cell line SGC7901, c-Met expression was detected using RT-PCR and Western blot, and was suppressed with lentivirus-mediated RNAi. The proliferation of SGC7901 cells was measured using MTr assay, and the invasion ability was detected with invasion assay. The adhesion of SGC7901 cells to peritoneum was observed in human peritoneal mesothelial cells （HPMCs） monolayer in vitro and in mice in vivo. Results： PFCCs were isolated from ascites of 6 out of 10 gastric cancer patients, c-Met expression in PFCCs was detected in 5 of the 6 gastric cancer patients. In SGC7901 cells, Lentivirus-mediated RNAi significantly reduced both c-Met mRNA and protein expression, which resulted in suppressing the cell proliferation, invasion and adhesion to peritoneum. The expression of a3131 integrin and E-cadherin was significantly inhibited in SGC7901 cells transfected with Lenti-miRNAc-Met. In the peritoneal dissemination model of gastric cancer, intraperitoneal injection of Lenti-miRNAc-Met markedly suppressed the tumor progression of SGC7901 cells. Conclusion： c-Met is expressed in PFCCs from the ascites of gastric cancer patients. Down-regulation of c-Met expression markedly suppresses the multistep process of peritoneal dissemination, thus may be a potential target for the treatment of gastric cancer.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>22407230</pmid><doi>10.1038/aps.2011.205</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal models Animals Ascites Biomedical and Life Sciences Biomedicine Cadherins - genetics Cell Adhesion Cell Line, Tumor Cell Proliferation Down-Regulation E-Cadherin Female Gastric cancer Gene expression Humans Immunocytochemistry Immunology Integrins Internal Medicine Lentivirus - genetics Medical Microbiology Mice Mice, Inbred BALB C Mice, Nude mRNA Neoplasm Invasiveness - genetics Neoplasm Invasiveness - prevention & control Original original-article Peritoneum Peritoneum - cytology Peritoneum - pathology Pharmacology/Toxicology Polymerase chain reaction Proto-Oncogene Proteins c-met - genetics RNA Interference RNA-mediated interference RNA沉默 Stomach - metabolism Stomach - pathology Stomach Neoplasms - genetics Stomach Neoplasms - pathology Tumor cell lines Tumor Cells, Cultured Tumors Vaccine Western blotting 介导 体内 体外 化学检测 慢病毒 胃癌细胞 腹膜 |
| title | Lentivirus-mediated RNA silencing of c-Met markedly suppresses peritoneal dissemination of gastric cancer in vitro and in vivo |
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