Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats
Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were us...
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| Veröffentlicht in: | BioMed research international 2014-01, Vol.2014 (2014), p.1-8 |
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| creator | Kraemer, Bernhard Wallwiener, Christian Rajab, Taufiek K. Brochhausen, Christoph Wallwiener, Markus Rothmund, Ralf |
| description | Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns (group 1), brushing of parietal peritoneum only (group 2), sharp excision of parietal peritoneum closed with interrupted sutures (group 3), ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture (group 4), bipolar electrocoagulation of the peritoneum (group 5), and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures (group 6). Upon second look, there were significant differences in the adhesion incidence between the groups (P |
| doi_str_mv | 10.1155/2014/435056 |
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We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns (group 1), brushing of parietal peritoneum only (group 2), sharp excision of parietal peritoneum closed with interrupted sutures (group 3), ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture (group 4), bipolar electrocoagulation of the peritoneum (group 5), and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures (group 6). Upon second look, there were significant differences in the adhesion incidence between the groups (P<0.01). Analysis of the fraction of adhesions showed that groups 2 (0%) and 5 (4%) were significantly less than the other groups (P<0.01). Furthermore, group 6 (69%) was significantly higher than group 1 (48%) (P<0.05) and group 4 (47%) (P<0.05). There was no difference between group 3 (60%) and group 6 (P=0.2). From a clinical viewpoint, comparison of different electrocoagulation modes and pharmaceutical adhesion barriers is possible with standardised models.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2014/435056</identifier><identifier>PMID: 24809049</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Abdomen ; Abdominal Wall - pathology ; Adhesion ; Animals ; Female ; Foreign-Body Reaction - pathology ; Hospitals ; Ischemia ; Laboratories ; Models, Biological ; Peritoneum - pathology ; Rats ; Rats, Wistar ; Surgery ; Sutures ; Tissue Adhesions - pathology ; Trauma</subject><ispartof>BioMed research international, 2014-01, Vol.2014 (2014), p.1-8</ispartof><rights>Copyright © 2014 Bernhard Kraemer et al.</rights><rights>Copyright © 2014 Bernhard Kraemer et al. Bernhard Kraemer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2014 Bernhard Kraemer et al. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-3743342663fa0be259786082650d9138fa20a0dc4800fb47112a70301a6a51bc3</citedby><cites>FETCH-LOGICAL-c499t-3743342663fa0be259786082650d9138fa20a0dc4800fb47112a70301a6a51bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997962/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997962/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24809049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Trew, Geoffrey</contributor><creatorcontrib>Kraemer, Bernhard</creatorcontrib><creatorcontrib>Wallwiener, Christian</creatorcontrib><creatorcontrib>Rajab, Taufiek K.</creatorcontrib><creatorcontrib>Brochhausen, Christoph</creatorcontrib><creatorcontrib>Wallwiener, Markus</creatorcontrib><creatorcontrib>Rothmund, Ralf</creatorcontrib><title>Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns (group 1), brushing of parietal peritoneum only (group 2), sharp excision of parietal peritoneum closed with interrupted sutures (group 3), ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture (group 4), bipolar electrocoagulation of the peritoneum (group 5), and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures (group 6). Upon second look, there were significant differences in the adhesion incidence between the groups (P<0.01). Analysis of the fraction of adhesions showed that groups 2 (0%) and 5 (4%) were significantly less than the other groups (P<0.01). Furthermore, group 6 (69%) was significantly higher than group 1 (48%) (P<0.05) and group 4 (47%) (P<0.05). There was no difference between group 3 (60%) and group 6 (P=0.2). From a clinical viewpoint, comparison of different electrocoagulation modes and pharmaceutical adhesion barriers is possible with standardised models.</description><subject>Abdomen</subject><subject>Abdominal Wall - pathology</subject><subject>Adhesion</subject><subject>Animals</subject><subject>Female</subject><subject>Foreign-Body Reaction - pathology</subject><subject>Hospitals</subject><subject>Ischemia</subject><subject>Laboratories</subject><subject>Models, Biological</subject><subject>Peritoneum - pathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Surgery</subject><subject>Sutures</subject><subject>Tissue Adhesions - pathology</subject><subject>Trauma</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkd1LHDEUxYNYVLY--dwy4Etp2XrzOZOXgoi2gqVf-hyyk4wbmU22yUyr_33vMnapfWnzkgv5cXLuOYQcUXhLqZQnDKg4EVyCVDvkgHEq5ooKurudOd8nh6XcAZ6GKtBqj-wz0YAGoQ_Il2-Djc5mF4p31cfkfF-qLuXqMrqxDfG2Or9f-xxWPg62rz7jOKTocTx1S19CiqUKsbrwK9v76qsdynPyrLN98YeP94zcXJxfn32YX316f3l2ejVvhdbDnNeCc8GU4p2FhWdS142ChikJTlPedJaBBdeiU-gWoqaU2Ro4UKuspIuWz8i7SXc9LlbetWgw296s0avNDybZYJ6-xLA0t-mH4VrXWjEUePUokNP30ZfBrEJpfd_b6NNYDJVMa7SFRv8D5QJAYtozcvwXepfGHDGJDYVbcKk21JuJanMqJftu65uC2RRrNsWaqVikX_656pb9XSMCrydgGbDMn-Efai8mGDvDn-0WxpB1I_kvMdaxuw</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Kraemer, Bernhard</creator><creator>Wallwiener, Christian</creator><creator>Rajab, Taufiek K.</creator><creator>Brochhausen, Christoph</creator><creator>Wallwiener, Markus</creator><creator>Rothmund, Ralf</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats</title><author>Kraemer, Bernhard ; Wallwiener, Christian ; Rajab, Taufiek K. ; Brochhausen, Christoph ; Wallwiener, Markus ; Rothmund, Ralf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-3743342663fa0be259786082650d9138fa20a0dc4800fb47112a70301a6a51bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abdomen</topic><topic>Abdominal Wall - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraemer, Bernhard</au><au>Wallwiener, Christian</au><au>Rajab, Taufiek K.</au><au>Brochhausen, Christoph</au><au>Wallwiener, Markus</au><au>Rothmund, Ralf</au><au>Trew, Geoffrey</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>2014</volume><issue>2014</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Animal models for adhesion induction are heterogeneous and often poorly described. We compare and discuss different models to induce peritoneal adhesions in a randomized, experimental in vivo animal study with 72 female Wistar rats. Six different standardized techniques for peritoneal trauma were used: brushing of peritoneal sidewall and uterine horns (group 1), brushing of parietal peritoneum only (group 2), sharp excision of parietal peritoneum closed with interrupted sutures (group 3), ischemic buttons by grasping the parietal peritoneum and ligating the base with Vicryl suture (group 4), bipolar electrocoagulation of the peritoneum (group 5), and traumatisation by electrocoagulation followed by closure of the resulting peritoneal defect using Vicryl sutures (group 6). Upon second look, there were significant differences in the adhesion incidence between the groups (P<0.01). Analysis of the fraction of adhesions showed that groups 2 (0%) and 5 (4%) were significantly less than the other groups (P<0.01). Furthermore, group 6 (69%) was significantly higher than group 1 (48%) (P<0.05) and group 4 (47%) (P<0.05). There was no difference between group 3 (60%) and group 6 (P=0.2). From a clinical viewpoint, comparison of different electrocoagulation modes and pharmaceutical adhesion barriers is possible with standardised models.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>24809049</pmid><doi>10.1155/2014/435056</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Abdominal Wall - pathology Adhesion Animals Female Foreign-Body Reaction - pathology Hospitals Ischemia Laboratories Models, Biological Peritoneum - pathology Rats Rats, Wistar Surgery Sutures Tissue Adhesions - pathology Trauma |
| title | Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats |
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