Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is a hypervascular tumor and accumulating evidence suggests that angiogenesis plays an important role in HCC development. Cordycepin, also known as 3′-deoxyadenosine, is a derivative of adenosine, and numerous cellular enzymes cannot differentiate the two. The aim of t...
Gespeichert in:
| Veröffentlicht in: | Oncology letters 2014-05, Vol.7 (5), p.1556-1562 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1562 |
|---|---|
| container_issue | 5 |
| container_start_page | 1556 |
| container_title | Oncology letters |
| container_volume | 7 |
| creator | LU, HAISHENG LI, XITING ZHANG, JIANYING SHI, HUI ZHU, XIAOFENG HE, XIAOSHUN |
| description | Hepatocellular carcinoma (HCC) is a hypervascular tumor and accumulating evidence suggests that angiogenesis plays an important role in HCC development. Cordycepin, also known as 3′-deoxyadenosine, is a derivative of adenosine, and numerous cellular enzymes cannot differentiate the two. The aim of the present study was to determine whether cordycepin regulates proliferation, migration and angiogenesis in a human umbilical vein endothelial cell line (EA.hy926) and in a hepatocellular carcinoma cell line (HepG2). MTT was used to assess cell proliferation. Apoptosis was analyzed by flow cytometry (propidium iodide staining). Transwell and wound healing assays were used to analyze the migration and invasion of HepG2 and EA.hy926 cells. Angiogenesis in EA.hy926 cells was assessed using a tube formation assay. Cordycepin strongly suppressed HepG2 and EA.hy926 cell proliferation in a dose- and time-dependent manner. Cordycepin induced EA.hy926 cell apoptosis in a dose-dependent manner (2,000 μg/ml: 50.20±1.55% vs. 0 μg/ml: 2.62±0.19%; P |
| doi_str_mv | 10.3892/ol.2014.1965 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3997733</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A415562790</galeid><sourcerecordid>A415562790</sourcerecordid><originalsourceid>FETCH-LOGICAL-c541t-2ed8cb4568920abebb08ee30a4b5539fa557226877efc04a6f4d84bec391c5393</originalsourceid><addsrcrecordid>eNptkk9r3DAQxU1paUKaW8_FUCg9xK4kW7LVQ2EJ26QQaA_tWcjyeFdBllxJDuwX6eetnD-72VL5IPP0mzfM8LLsLUZl1XLyyZmSIFyXmDP6IjvFDScFRi15uf9v6pPsPIRblA5luG3Z6-yE1A2juKGn2Z_1MICKIXdDrpzvdwombXNn82uYrkgubZ-vV-V2xwnLFRgTPuc_XAQbtTTpNerJO6MH8DLqO7i4l0aIMsQkqPv6RSqk3Wi3AZs0eGpp8y1MMrrFdzbS50p6pa0b5Zvs1SBNgPPH-yz79XX98_K6uPl-9e1ydVMoWuNYEOhb1dWUpV0g2UHXoRagQrLuKK34ICltCGFt08CgUC3ZUPdt3YGqOFYJqM6yLw--09yN0Ks0l5dGTF6P0u-Ek1ocv1i9FRt3JyrOm6aqksHHRwPvfs8Qohh1WOaRFtwcBG4Joy3jqE3o-3_QWzd7m8YTmFeE1ZQjdKA20oDQdnCpr1pMxarGlDLS8IUq_0Olr4dRK2dh0Ek_KvjwrGAL0sRtcGaO2tlwDF48gMq7EDwM-2VgJJbMCWfEkjmxZC7h754vcA8_JezQOEwpC7p34bBdU6CmQLRYmld_ASwe3n0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932645900</pqid></control><display><type>article</type><title>Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma</title><source>Spandidos Publications Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>LU, HAISHENG ; LI, XITING ; ZHANG, JIANYING ; SHI, HUI ; ZHU, XIAOFENG ; HE, XIAOSHUN</creator><creatorcontrib>LU, HAISHENG ; LI, XITING ; ZHANG, JIANYING ; SHI, HUI ; ZHU, XIAOFENG ; HE, XIAOSHUN</creatorcontrib><description>Hepatocellular carcinoma (HCC) is a hypervascular tumor and accumulating evidence suggests that angiogenesis plays an important role in HCC development. Cordycepin, also known as 3′-deoxyadenosine, is a derivative of adenosine, and numerous cellular enzymes cannot differentiate the two. The aim of the present study was to determine whether cordycepin regulates proliferation, migration and angiogenesis in a human umbilical vein endothelial cell line (EA.hy926) and in a hepatocellular carcinoma cell line (HepG2). MTT was used to assess cell proliferation. Apoptosis was analyzed by flow cytometry (propidium iodide staining). Transwell and wound healing assays were used to analyze the migration and invasion of HepG2 and EA.hy926 cells. Angiogenesis in EA.hy926 cells was assessed using a tube formation assay. Cordycepin strongly suppressed HepG2 and EA.hy926 cell proliferation in a dose- and time-dependent manner. Cordycepin induced EA.hy926 cell apoptosis in a dose-dependent manner (2,000 μg/ml: 50.20±1.55% vs. 0 μg/ml: 2.62±0.19%; P<0.01). Cordycepin inhibited EA.hy926 cell migration (percentage of wound healing area, 2,000 μg/ml: 3.45±0.29% vs. 0 μg/ml: 85.48±0.84%; P<0.05), as well as tube formation (total length of tubular structure, 1,000 μg/ml: 107±39 μm vs. 0 μg/ml: 936±56 μm; P<0.05). Cordycepin also efficiently inhibited HepG2 cell invasion and migration. High-performance liquid chromatography analysis of the cytosol from EA.hy926 cells showed that cordycepin was stable for 3 h. In conclusion, cordycepin not only inhibited human HepG2 cell proliferation and invasion, but also induced apoptosis and inhibited migration and angiogenesis in vascular endothelial cells, suggesting that cordycepin may be used as a novel anti-angiogenic therapy in HCC.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2014.1965</identifier><identifier>PMID: 24765175</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Adenosine ; Analysis ; Angiogenesis ; Apoptosis ; Cancer therapies ; Care and treatment ; Cell adhesion & migration ; Cell growth ; cordycepin ; Development and progression ; Genetic aspects ; hepatocellular carcinoma ; Hepatoma ; invasion ; Liver cancer ; Medical prognosis ; Metastasis ; Oncology ; Penicillin ; Rodents ; Studies ; vascular endothelial cells</subject><ispartof>Oncology letters, 2014-05, Vol.7 (5), p.1556-1562</ispartof><rights>Copyright © 2014, Spandidos Publications</rights><rights>COPYRIGHT 2014 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2014</rights><rights>Copyright © 2014, Spandidos Publications 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-2ed8cb4568920abebb08ee30a4b5539fa557226877efc04a6f4d84bec391c5393</citedby><cites>FETCH-LOGICAL-c541t-2ed8cb4568920abebb08ee30a4b5539fa557226877efc04a6f4d84bec391c5393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997733/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997733/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,5556,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24765175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LU, HAISHENG</creatorcontrib><creatorcontrib>LI, XITING</creatorcontrib><creatorcontrib>ZHANG, JIANYING</creatorcontrib><creatorcontrib>SHI, HUI</creatorcontrib><creatorcontrib>ZHU, XIAOFENG</creatorcontrib><creatorcontrib>HE, XIAOSHUN</creatorcontrib><title>Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Hepatocellular carcinoma (HCC) is a hypervascular tumor and accumulating evidence suggests that angiogenesis plays an important role in HCC development. Cordycepin, also known as 3′-deoxyadenosine, is a derivative of adenosine, and numerous cellular enzymes cannot differentiate the two. The aim of the present study was to determine whether cordycepin regulates proliferation, migration and angiogenesis in a human umbilical vein endothelial cell line (EA.hy926) and in a hepatocellular carcinoma cell line (HepG2). MTT was used to assess cell proliferation. Apoptosis was analyzed by flow cytometry (propidium iodide staining). Transwell and wound healing assays were used to analyze the migration and invasion of HepG2 and EA.hy926 cells. Angiogenesis in EA.hy926 cells was assessed using a tube formation assay. Cordycepin strongly suppressed HepG2 and EA.hy926 cell proliferation in a dose- and time-dependent manner. Cordycepin induced EA.hy926 cell apoptosis in a dose-dependent manner (2,000 μg/ml: 50.20±1.55% vs. 0 μg/ml: 2.62±0.19%; P<0.01). Cordycepin inhibited EA.hy926 cell migration (percentage of wound healing area, 2,000 μg/ml: 3.45±0.29% vs. 0 μg/ml: 85.48±0.84%; P<0.05), as well as tube formation (total length of tubular structure, 1,000 μg/ml: 107±39 μm vs. 0 μg/ml: 936±56 μm; P<0.05). Cordycepin also efficiently inhibited HepG2 cell invasion and migration. High-performance liquid chromatography analysis of the cytosol from EA.hy926 cells showed that cordycepin was stable for 3 h. In conclusion, cordycepin not only inhibited human HepG2 cell proliferation and invasion, but also induced apoptosis and inhibited migration and angiogenesis in vascular endothelial cells, suggesting that cordycepin may be used as a novel anti-angiogenic therapy in HCC.</description><subject>Adenosine</subject><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>cordycepin</subject><subject>Development and progression</subject><subject>Genetic aspects</subject><subject>hepatocellular carcinoma</subject><subject>Hepatoma</subject><subject>invasion</subject><subject>Liver cancer</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Oncology</subject><subject>Penicillin</subject><subject>Rodents</subject><subject>Studies</subject><subject>vascular endothelial cells</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNptkk9r3DAQxU1paUKaW8_FUCg9xK4kW7LVQ2EJ26QQaA_tWcjyeFdBllxJDuwX6eetnD-72VL5IPP0mzfM8LLsLUZl1XLyyZmSIFyXmDP6IjvFDScFRi15uf9v6pPsPIRblA5luG3Z6-yE1A2juKGn2Z_1MICKIXdDrpzvdwombXNn82uYrkgubZ-vV-V2xwnLFRgTPuc_XAQbtTTpNerJO6MH8DLqO7i4l0aIMsQkqPv6RSqk3Wi3AZs0eGpp8y1MMrrFdzbS50p6pa0b5Zvs1SBNgPPH-yz79XX98_K6uPl-9e1ydVMoWuNYEOhb1dWUpV0g2UHXoRagQrLuKK34ICltCGFt08CgUC3ZUPdt3YGqOFYJqM6yLw--09yN0Ks0l5dGTF6P0u-Ek1ocv1i9FRt3JyrOm6aqksHHRwPvfs8Qohh1WOaRFtwcBG4Joy3jqE3o-3_QWzd7m8YTmFeE1ZQjdKA20oDQdnCpr1pMxarGlDLS8IUq_0Olr4dRK2dh0Ek_KvjwrGAL0sRtcGaO2tlwDF48gMq7EDwM-2VgJJbMCWfEkjmxZC7h754vcA8_JezQOEwpC7p34bBdU6CmQLRYmld_ASwe3n0</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>LU, HAISHENG</creator><creator>LI, XITING</creator><creator>ZHANG, JIANYING</creator><creator>SHI, HUI</creator><creator>ZHU, XIAOFENG</creator><creator>HE, XIAOSHUN</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140501</creationdate><title>Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma</title><author>LU, HAISHENG ; LI, XITING ; ZHANG, JIANYING ; SHI, HUI ; ZHU, XIAOFENG ; HE, XIAOSHUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-2ed8cb4568920abebb08ee30a4b5539fa557226877efc04a6f4d84bec391c5393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenosine</topic><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>cordycepin</topic><topic>Development and progression</topic><topic>Genetic aspects</topic><topic>hepatocellular carcinoma</topic><topic>Hepatoma</topic><topic>invasion</topic><topic>Liver cancer</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Oncology</topic><topic>Penicillin</topic><topic>Rodents</topic><topic>Studies</topic><topic>vascular endothelial cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LU, HAISHENG</creatorcontrib><creatorcontrib>LI, XITING</creatorcontrib><creatorcontrib>ZHANG, JIANYING</creatorcontrib><creatorcontrib>SHI, HUI</creatorcontrib><creatorcontrib>ZHU, XIAOFENG</creatorcontrib><creatorcontrib>HE, XIAOSHUN</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LU, HAISHENG</au><au>LI, XITING</au><au>ZHANG, JIANYING</au><au>SHI, HUI</au><au>ZHU, XIAOFENG</au><au>HE, XIAOSHUN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>7</volume><issue>5</issue><spage>1556</spage><epage>1562</epage><pages>1556-1562</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>Hepatocellular carcinoma (HCC) is a hypervascular tumor and accumulating evidence suggests that angiogenesis plays an important role in HCC development. Cordycepin, also known as 3′-deoxyadenosine, is a derivative of adenosine, and numerous cellular enzymes cannot differentiate the two. The aim of the present study was to determine whether cordycepin regulates proliferation, migration and angiogenesis in a human umbilical vein endothelial cell line (EA.hy926) and in a hepatocellular carcinoma cell line (HepG2). MTT was used to assess cell proliferation. Apoptosis was analyzed by flow cytometry (propidium iodide staining). Transwell and wound healing assays were used to analyze the migration and invasion of HepG2 and EA.hy926 cells. Angiogenesis in EA.hy926 cells was assessed using a tube formation assay. Cordycepin strongly suppressed HepG2 and EA.hy926 cell proliferation in a dose- and time-dependent manner. Cordycepin induced EA.hy926 cell apoptosis in a dose-dependent manner (2,000 μg/ml: 50.20±1.55% vs. 0 μg/ml: 2.62±0.19%; P<0.01). Cordycepin inhibited EA.hy926 cell migration (percentage of wound healing area, 2,000 μg/ml: 3.45±0.29% vs. 0 μg/ml: 85.48±0.84%; P<0.05), as well as tube formation (total length of tubular structure, 1,000 μg/ml: 107±39 μm vs. 0 μg/ml: 936±56 μm; P<0.05). Cordycepin also efficiently inhibited HepG2 cell invasion and migration. High-performance liquid chromatography analysis of the cytosol from EA.hy926 cells showed that cordycepin was stable for 3 h. In conclusion, cordycepin not only inhibited human HepG2 cell proliferation and invasion, but also induced apoptosis and inhibited migration and angiogenesis in vascular endothelial cells, suggesting that cordycepin may be used as a novel anti-angiogenic therapy in HCC.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>24765175</pmid><doi>10.3892/ol.2014.1965</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1792-1074 |
| ispartof | Oncology letters, 2014-05, Vol.7 (5), p.1556-1562 |
| issn | 1792-1074 1792-1082 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3997733 |
| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adenosine Analysis Angiogenesis Apoptosis Cancer therapies Care and treatment Cell adhesion & migration Cell growth cordycepin Development and progression Genetic aspects hepatocellular carcinoma Hepatoma invasion Liver cancer Medical prognosis Metastasis Oncology Penicillin Rodents Studies vascular endothelial cells |
| title | Effects of cordycepin on HepG2 and EA.hy926 cells: Potential antiproliferative, antimetastatic and anti-angiogenic effects on hepatocellular carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T08%3A16%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20cordycepin%20on%20HepG2%20and%20EA.hy926%20cells:%20Potential%20antiproliferative,%20antimetastatic%20and%20anti-angiogenic%20effects%20on%20hepatocellular%20carcinoma&rft.jtitle=Oncology%20letters&rft.au=LU,%20HAISHENG&rft.date=2014-05-01&rft.volume=7&rft.issue=5&rft.spage=1556&rft.epage=1562&rft.pages=1556-1562&rft.issn=1792-1074&rft.eissn=1792-1082&rft_id=info:doi/10.3892/ol.2014.1965&rft_dat=%3Cgale_pubme%3EA415562790%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932645900&rft_id=info:pmid/24765175&rft_galeid=A415562790&rfr_iscdi=true |