A dual chicken IgY against rotavirus and norovirus

► IgY against both RV and NoV were produced by immunizing chickens with a dual vaccine candidate P-VP8∗. ► The resulting IgY blocked RV and NoV binding to their HBGA receptors. ► The IgY is also able to neutralize RV replication in cell culture condition. ► The P-VP8∗ based IgY is a promising approach for passive immunization against both RV and NoV. Rotavirus (RV) and norovirus (NoV) are the two most important causes of viral gastroenteritis. While vaccine remains an effective prophylactic strategy, development of other approaches, such as passive immunization to control and treat clinical infection and illness of these two pathogens, is necessary. Previously we demonstrated that high titers of NoV-specific IgY were readily developed by immunization of chickens with the NoV P particles. In this study, we developed a dual IgY against both RV and NoV through immunization of chickens with a divalent vaccine comprising neutralizing antigens of both RV and NoV. This divalent vaccine, named P-VP8∗ particle, is made of the NoV P particle as a carrier with the RV spike protein VP8∗ as a surface insertion. Approximately 45mg of IgY were readily obtained from each yolk with high titers of anti-P particle and anti-VP8∗ antibodies detected by ELISA, Western blot, HBGA blocking (NoV and RV) and neutralization (RV) assays. Reductions of RV replication were observed with viruses treated with the IgY before and after inoculation into cells, suggesting an application of the IgY as both prophylactic and therapeutic treatment. Collectively, our data suggested that the P-VP8∗ based IgY could serve as a practical approach against both NoV and RV.