PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model)
Background and objectivesPreImplantation factor (PIF) secreted by viable embryos exerts essential regulatory role on global systemic immune response. Synthetic PIF (PIF) translates endogenous effects to immune disorder models. Metastatic melanoma displays tumor-immunological behaviour. Static magnet...
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| creator | Kotlan, Beatrix Laszlo, Janos F Tovari, Jozsef Kasler, Miklos Barnea, Eytan |
| description | Background and objectivesPreImplantation factor (PIF) secreted by viable embryos exerts essential regulatory role on global systemic immune response. Synthetic PIF (PIF) translates endogenous effects to immune disorder models. Metastatic melanoma displays tumor-immunological behaviour. Static magnetic field (SMF) affects inflammatory reactions. There is increased interest toward SMF’s potential anti-tumor effects. Herein examined a novel anti-melanoma strategy using combined physical and immune-based therapy.MethodsDaily whole-body SMF exposure, combined with subcutaneous PIF administration was examined in engrafted HT199 melanoma cells’ progression, transplanted into NSG mice. PIF effect on unique tumor-associated antigen expression relevant for tumor proliferation/ invasion was examined in vitro using specific antibodies. Direct PIF anti-proliferative effects on several cancer cell lines were tested using MTT.ResultsPIF potentiates SMF beneficial effect by reducing tumor volume vs. control (Mmax=96%) on day 34. Metastatic spleen mass is reduced by SMF alone (M=59%) or combined with PIF (M=62%). Daily SMF exposure alone inhibits tumor outgrowth (Mmax=60%, F5.32 (P |
| doi_str_mv | 10.1186/2051-1426-1-S1-P80 |
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| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3991376</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2638100563</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1700-d74827c5d733155dab607e32b0b90248094234ca75e61f60f1eecbabefd136d03</originalsourceid><addsrcrecordid>eNpVkUFr3DAQhU1poEuyf6AnQS67B6czli17L4GyNOlCSha2PQtZGm0dLGsryYFAf3xtNoT0NI-ZxzczvCz7jHCD2IgvBVSYY1mIHPMD5vsGPmSLt-bHd_pTtozxCQAQOG-aZpH93QfauVOvhqRS5wdmlU4-sNV-d7dmJ59oSJ1KFFmcDZo5dRxoFraj3rDV4cfkI2tJJ5Y8M6QDqUgsjW7CtGMwNLCVo2mDd4q5MXQDMecN9eur7MKqPtLytV5mv-6-_dx-zx8e73fbrw-5xhogN3XZFLWuTM05VpVRrYCaeNFCu4GibGBTFrzUqq5IoBVgkUi3qiVrkAsD_DK7PXNPY-vI6OmnoHp5Cp1T4UV61cn_J0P3Wx79s-SbDfJaTIDrV0Dwf0aKST75MQzTzbIQvEGASvDJVZxdOvgYA9m3DQhyTkrOQcg5CInygHJKiv8DCsOHtQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2638100563</pqid></control><display><type>article</type><title>PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model)</title><source>BMJ Open Access Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Kotlan, Beatrix ; Laszlo, Janos F ; Tovari, Jozsef ; Kasler, Miklos ; Barnea, Eytan</creator><creatorcontrib>Kotlan, Beatrix ; Laszlo, Janos F ; Tovari, Jozsef ; Kasler, Miklos ; Barnea, Eytan</creatorcontrib><description>Background and objectivesPreImplantation factor (PIF) secreted by viable embryos exerts essential regulatory role on global systemic immune response. Synthetic PIF (PIF) translates endogenous effects to immune disorder models. Metastatic melanoma displays tumor-immunological behaviour. Static magnetic field (SMF) affects inflammatory reactions. There is increased interest toward SMF’s potential anti-tumor effects. Herein examined a novel anti-melanoma strategy using combined physical and immune-based therapy.MethodsDaily whole-body SMF exposure, combined with subcutaneous PIF administration was examined in engrafted HT199 melanoma cells’ progression, transplanted into NSG mice. PIF effect on unique tumor-associated antigen expression relevant for tumor proliferation/ invasion was examined in vitro using specific antibodies. Direct PIF anti-proliferative effects on several cancer cell lines were tested using MTT.ResultsPIF potentiates SMF beneficial effect by reducing tumor volume vs. control (Mmax=96%) on day 34. Metastatic spleen mass is reduced by SMF alone (M=59%) or combined with PIF (M=62%). Daily SMF exposure alone inhibits tumor outgrowth (Mmax=60%, F5.32 (P<0.002)=21.16) while in combination with PIF, effect is considerably potentiated (M=80%), F5.32(P<0.0004)=34.84). PIF did not impair tumor antigen expression nor reduced significantly cultured tumor cell lines’ proliferation.ConclusionsCollectively, results indicate that PIF’s potentiating anti-tumoral effect is mainly immune-regulatory, synergizing with SMF’s pro-tumor necrosis properties. The preserved tumor-associated antigen expression is important for the maintained antitumor immune activity. Overall, combined physico / immune regulatory treatment represents a useful, promising novel avenue for anti-cancer strategy.</description><identifier>ISSN: 2051-1426</identifier><identifier>EISSN: 2051-1426</identifier><identifier>DOI: 10.1186/2051-1426-1-S1-P80</identifier><language>eng</language><publisher>London: BMJ Publishing Group LTD</publisher><subject>Antigens ; Immunotherapy ; Melanoma ; Metastasis ; Poster Presentation</subject><ispartof>Journal for immunotherapy of cancer, 2013-11, Vol.1 (S1), p.P80-P80, Article P80</ispartof><rights>2013 Kotlan et al; licensee BioMed Central Ltd. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2013 Kotlan et al; licensee BioMed Central Ltd. 2013 Kotlan et al; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991376/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991376/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Kotlan, Beatrix</creatorcontrib><creatorcontrib>Laszlo, Janos F</creatorcontrib><creatorcontrib>Tovari, Jozsef</creatorcontrib><creatorcontrib>Kasler, Miklos</creatorcontrib><creatorcontrib>Barnea, Eytan</creatorcontrib><title>PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model)</title><title>Journal for immunotherapy of cancer</title><description>Background and objectivesPreImplantation factor (PIF) secreted by viable embryos exerts essential regulatory role on global systemic immune response. Synthetic PIF (PIF) translates endogenous effects to immune disorder models. Metastatic melanoma displays tumor-immunological behaviour. Static magnetic field (SMF) affects inflammatory reactions. There is increased interest toward SMF’s potential anti-tumor effects. Herein examined a novel anti-melanoma strategy using combined physical and immune-based therapy.MethodsDaily whole-body SMF exposure, combined with subcutaneous PIF administration was examined in engrafted HT199 melanoma cells’ progression, transplanted into NSG mice. PIF effect on unique tumor-associated antigen expression relevant for tumor proliferation/ invasion was examined in vitro using specific antibodies. Direct PIF anti-proliferative effects on several cancer cell lines were tested using MTT.ResultsPIF potentiates SMF beneficial effect by reducing tumor volume vs. control (Mmax=96%) on day 34. Metastatic spleen mass is reduced by SMF alone (M=59%) or combined with PIF (M=62%). Daily SMF exposure alone inhibits tumor outgrowth (Mmax=60%, F5.32 (P<0.002)=21.16) while in combination with PIF, effect is considerably potentiated (M=80%), F5.32(P<0.0004)=34.84). PIF did not impair tumor antigen expression nor reduced significantly cultured tumor cell lines’ proliferation.ConclusionsCollectively, results indicate that PIF’s potentiating anti-tumoral effect is mainly immune-regulatory, synergizing with SMF’s pro-tumor necrosis properties. The preserved tumor-associated antigen expression is important for the maintained antitumor immune activity. Overall, combined physico / immune regulatory treatment represents a useful, promising novel avenue for anti-cancer strategy.</description><subject>Antigens</subject><subject>Immunotherapy</subject><subject>Melanoma</subject><subject>Metastasis</subject><subject>Poster Presentation</subject><issn>2051-1426</issn><issn>2051-1426</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpVkUFr3DAQhU1poEuyf6AnQS67B6czli17L4GyNOlCSha2PQtZGm0dLGsryYFAf3xtNoT0NI-ZxzczvCz7jHCD2IgvBVSYY1mIHPMD5vsGPmSLt-bHd_pTtozxCQAQOG-aZpH93QfauVOvhqRS5wdmlU4-sNV-d7dmJ59oSJ1KFFmcDZo5dRxoFraj3rDV4cfkI2tJJ5Y8M6QDqUgsjW7CtGMwNLCVo2mDd4q5MXQDMecN9eur7MKqPtLytV5mv-6-_dx-zx8e73fbrw-5xhogN3XZFLWuTM05VpVRrYCaeNFCu4GibGBTFrzUqq5IoBVgkUi3qiVrkAsD_DK7PXNPY-vI6OmnoHp5Cp1T4UV61cn_J0P3Wx79s-SbDfJaTIDrV0Dwf0aKST75MQzTzbIQvEGASvDJVZxdOvgYA9m3DQhyTkrOQcg5CInygHJKiv8DCsOHtQ</recordid><startdate>20131107</startdate><enddate>20131107</enddate><creator>Kotlan, Beatrix</creator><creator>Laszlo, Janos F</creator><creator>Tovari, Jozsef</creator><creator>Kasler, Miklos</creator><creator>Barnea, Eytan</creator><general>BMJ Publishing Group LTD</general><general>BioMed Central</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20131107</creationdate><title>PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model)</title><author>Kotlan, Beatrix ; Laszlo, Janos F ; Tovari, Jozsef ; Kasler, Miklos ; Barnea, Eytan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1700-d74827c5d733155dab607e32b0b90248094234ca75e61f60f1eecbabefd136d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antigens</topic><topic>Immunotherapy</topic><topic>Melanoma</topic><topic>Metastasis</topic><topic>Poster Presentation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kotlan, Beatrix</creatorcontrib><creatorcontrib>Laszlo, Janos F</creatorcontrib><creatorcontrib>Tovari, Jozsef</creatorcontrib><creatorcontrib>Kasler, Miklos</creatorcontrib><creatorcontrib>Barnea, Eytan</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal for immunotherapy of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kotlan, Beatrix</au><au>Laszlo, Janos F</au><au>Tovari, Jozsef</au><au>Kasler, Miklos</au><au>Barnea, Eytan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model)</atitle><jtitle>Journal for immunotherapy of cancer</jtitle><date>2013-11-07</date><risdate>2013</risdate><volume>1</volume><issue>S1</issue><spage>P80</spage><epage>P80</epage><pages>P80-P80</pages><artnum>P80</artnum><issn>2051-1426</issn><eissn>2051-1426</eissn><abstract>Background and objectivesPreImplantation factor (PIF) secreted by viable embryos exerts essential regulatory role on global systemic immune response. Synthetic PIF (PIF) translates endogenous effects to immune disorder models. Metastatic melanoma displays tumor-immunological behaviour. Static magnetic field (SMF) affects inflammatory reactions. There is increased interest toward SMF’s potential anti-tumor effects. Herein examined a novel anti-melanoma strategy using combined physical and immune-based therapy.MethodsDaily whole-body SMF exposure, combined with subcutaneous PIF administration was examined in engrafted HT199 melanoma cells’ progression, transplanted into NSG mice. PIF effect on unique tumor-associated antigen expression relevant for tumor proliferation/ invasion was examined in vitro using specific antibodies. Direct PIF anti-proliferative effects on several cancer cell lines were tested using MTT.ResultsPIF potentiates SMF beneficial effect by reducing tumor volume vs. control (Mmax=96%) on day 34. Metastatic spleen mass is reduced by SMF alone (M=59%) or combined with PIF (M=62%). Daily SMF exposure alone inhibits tumor outgrowth (Mmax=60%, F5.32 (P<0.002)=21.16) while in combination with PIF, effect is considerably potentiated (M=80%), F5.32(P<0.0004)=34.84). PIF did not impair tumor antigen expression nor reduced significantly cultured tumor cell lines’ proliferation.ConclusionsCollectively, results indicate that PIF’s potentiating anti-tumoral effect is mainly immune-regulatory, synergizing with SMF’s pro-tumor necrosis properties. The preserved tumor-associated antigen expression is important for the maintained antitumor immune activity. Overall, combined physico / immune regulatory treatment represents a useful, promising novel avenue for anti-cancer strategy.</abstract><cop>London</cop><pub>BMJ Publishing Group LTD</pub><doi>10.1186/2051-1426-1-S1-P80</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Immunotherapy Melanoma Metastasis Poster Presentation |
| title | PreImplantation factor (PIF) potentiates static magnetic field (SMF) effect to decrease tumor burden (melanoma murine model) |
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